Cargando…
Qualitative and quantitative analysis of bacterial aerosols in dental clinical settings: Risk exposure towards dentist, auxiliary staff, and patients
INTRODUCTION: Cross-infection or contamination are the major threats related to any medical profession. Microorganisms present in the dental clinic can cause cross-infection to the dentist, auxiliary staff and even towards the patients. AIMS: The study was conducted to assess the level of atmospheri...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113934/ https://www.ncbi.nlm.nih.gov/pubmed/32318458 http://dx.doi.org/10.4103/jfmpc.jfmpc_863_19 |
_version_ | 1783513774597079040 |
---|---|
author | Jain, Manish Mathur, Aditi Mathur, Anmol Mukhi, Pravin U. Ahire, Mahesh Pingal, Chadrashekhar |
author_facet | Jain, Manish Mathur, Aditi Mathur, Anmol Mukhi, Pravin U. Ahire, Mahesh Pingal, Chadrashekhar |
author_sort | Jain, Manish |
collection | PubMed |
description | INTRODUCTION: Cross-infection or contamination are the major threats related to any medical profession. Microorganisms present in the dental clinic can cause cross-infection to the dentist, auxiliary staff and even towards the patients. AIMS: The study was conducted to assess the level of atmospheric microbial contamination and composition of aerosols before, during and after dental treatment procedures in four clinical settings. METHODS AND MATERIALS: The present study was conducted over a two-week period in a private dental college setting. An equal number of culture medium plates (blood agar) were placed 30 min prior to the initiation of work sessions in the selected area and 1 h after the working session began and after 2 h of cessation of the working period. After the collection of samples, the culture medium plates were incubated aerobically at 37°C in an incubator for 48 h. The number of colonies was expressed as colonies per media plate. After counting the colonies bacterial cell morphology was determined by a microscopic examination using a Reichert-Jung Series 150 light microscope. STATISTICAL ANALYSIS: Statistical analysis such as ANOVA test for mean values and post hock was done using statistical package for social sciences (SPSS). RESULTS: It shows that colony count increased after the working session and which reduced by itself once the working session was concluded which was significant (P < 0.001). The highest increase in the mean colony count was found in the department of periodontology during the treatment sessions. In the blood agar plates, the S. epidermidis was found maximum 62%, micrococcus was 22%, diphtheroid was 10%, fungi 4% and the least S. aureus 2%. CONCLUSION: This study demonstrates that aerosols increase during and after work sessions and, therefore, increases the chance for infectious agent transmission in clinical settings. |
format | Online Article Text |
id | pubmed-7113934 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-71139342020-04-21 Qualitative and quantitative analysis of bacterial aerosols in dental clinical settings: Risk exposure towards dentist, auxiliary staff, and patients Jain, Manish Mathur, Aditi Mathur, Anmol Mukhi, Pravin U. Ahire, Mahesh Pingal, Chadrashekhar J Family Med Prim Care Original Article INTRODUCTION: Cross-infection or contamination are the major threats related to any medical profession. Microorganisms present in the dental clinic can cause cross-infection to the dentist, auxiliary staff and even towards the patients. AIMS: The study was conducted to assess the level of atmospheric microbial contamination and composition of aerosols before, during and after dental treatment procedures in four clinical settings. METHODS AND MATERIALS: The present study was conducted over a two-week period in a private dental college setting. An equal number of culture medium plates (blood agar) were placed 30 min prior to the initiation of work sessions in the selected area and 1 h after the working session began and after 2 h of cessation of the working period. After the collection of samples, the culture medium plates were incubated aerobically at 37°C in an incubator for 48 h. The number of colonies was expressed as colonies per media plate. After counting the colonies bacterial cell morphology was determined by a microscopic examination using a Reichert-Jung Series 150 light microscope. STATISTICAL ANALYSIS: Statistical analysis such as ANOVA test for mean values and post hock was done using statistical package for social sciences (SPSS). RESULTS: It shows that colony count increased after the working session and which reduced by itself once the working session was concluded which was significant (P < 0.001). The highest increase in the mean colony count was found in the department of periodontology during the treatment sessions. In the blood agar plates, the S. epidermidis was found maximum 62%, micrococcus was 22%, diphtheroid was 10%, fungi 4% and the least S. aureus 2%. CONCLUSION: This study demonstrates that aerosols increase during and after work sessions and, therefore, increases the chance for infectious agent transmission in clinical settings. Wolters Kluwer - Medknow 2020-02-28 /pmc/articles/PMC7113934/ /pubmed/32318458 http://dx.doi.org/10.4103/jfmpc.jfmpc_863_19 Text en Copyright: © Journal of Family Medicine and Primary Care http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Jain, Manish Mathur, Aditi Mathur, Anmol Mukhi, Pravin U. Ahire, Mahesh Pingal, Chadrashekhar Qualitative and quantitative analysis of bacterial aerosols in dental clinical settings: Risk exposure towards dentist, auxiliary staff, and patients |
title | Qualitative and quantitative analysis of bacterial aerosols in dental clinical settings: Risk exposure towards dentist, auxiliary staff, and patients |
title_full | Qualitative and quantitative analysis of bacterial aerosols in dental clinical settings: Risk exposure towards dentist, auxiliary staff, and patients |
title_fullStr | Qualitative and quantitative analysis of bacterial aerosols in dental clinical settings: Risk exposure towards dentist, auxiliary staff, and patients |
title_full_unstemmed | Qualitative and quantitative analysis of bacterial aerosols in dental clinical settings: Risk exposure towards dentist, auxiliary staff, and patients |
title_short | Qualitative and quantitative analysis of bacterial aerosols in dental clinical settings: Risk exposure towards dentist, auxiliary staff, and patients |
title_sort | qualitative and quantitative analysis of bacterial aerosols in dental clinical settings: risk exposure towards dentist, auxiliary staff, and patients |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113934/ https://www.ncbi.nlm.nih.gov/pubmed/32318458 http://dx.doi.org/10.4103/jfmpc.jfmpc_863_19 |
work_keys_str_mv | AT jainmanish qualitativeandquantitativeanalysisofbacterialaerosolsindentalclinicalsettingsriskexposuretowardsdentistauxiliarystaffandpatients AT mathuraditi qualitativeandquantitativeanalysisofbacterialaerosolsindentalclinicalsettingsriskexposuretowardsdentistauxiliarystaffandpatients AT mathuranmol qualitativeandquantitativeanalysisofbacterialaerosolsindentalclinicalsettingsriskexposuretowardsdentistauxiliarystaffandpatients AT mukhipravinu qualitativeandquantitativeanalysisofbacterialaerosolsindentalclinicalsettingsriskexposuretowardsdentistauxiliarystaffandpatients AT ahiremahesh qualitativeandquantitativeanalysisofbacterialaerosolsindentalclinicalsettingsriskexposuretowardsdentistauxiliarystaffandpatients AT pingalchadrashekhar qualitativeandquantitativeanalysisofbacterialaerosolsindentalclinicalsettingsriskexposuretowardsdentistauxiliarystaffandpatients |