Inhibition of human coronavirus 229E infection in human epithelial lung cells (L132) by chloroquine: Involvement of p38 MAPK and ERK

The antiviral effects of chloroquine (CQ) on human coronavirus 229E (HCoV-229E) infection of human fetal lung cell line, L132 are reported. CQ significantly decreased the viral replication at concentrations lower than in clinical usage. We demonstrated that CQ affects the activation of p38 mitogen-a...

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Autores principales: Kono, Masakazu, Tatsumi, Koichiro, Imai, Alberto M., Saito, Kengo, Kuriyama, Takayuki, Shirasawa, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114149/
https://www.ncbi.nlm.nih.gov/pubmed/18055026
http://dx.doi.org/10.1016/j.antiviral.2007.10.011
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author Kono, Masakazu
Tatsumi, Koichiro
Imai, Alberto M.
Saito, Kengo
Kuriyama, Takayuki
Shirasawa, Hiroshi
author_facet Kono, Masakazu
Tatsumi, Koichiro
Imai, Alberto M.
Saito, Kengo
Kuriyama, Takayuki
Shirasawa, Hiroshi
author_sort Kono, Masakazu
collection PubMed
description The antiviral effects of chloroquine (CQ) on human coronavirus 229E (HCoV-229E) infection of human fetal lung cell line, L132 are reported. CQ significantly decreased the viral replication at concentrations lower than in clinical usage. We demonstrated that CQ affects the activation of p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinase (ERK). Furthermore, p38 MAPK inhibitor, SB203580, inhibits CPE induced by HCoV-229E infection and viral replication. Our findings suggest that CQ affects the activation of MAPKs, involved in the replication of HCoV-229E.
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spelling pubmed-71141492020-04-02 Inhibition of human coronavirus 229E infection in human epithelial lung cells (L132) by chloroquine: Involvement of p38 MAPK and ERK Kono, Masakazu Tatsumi, Koichiro Imai, Alberto M. Saito, Kengo Kuriyama, Takayuki Shirasawa, Hiroshi Antiviral Res Article The antiviral effects of chloroquine (CQ) on human coronavirus 229E (HCoV-229E) infection of human fetal lung cell line, L132 are reported. CQ significantly decreased the viral replication at concentrations lower than in clinical usage. We demonstrated that CQ affects the activation of p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinase (ERK). Furthermore, p38 MAPK inhibitor, SB203580, inhibits CPE induced by HCoV-229E infection and viral replication. Our findings suggest that CQ affects the activation of MAPKs, involved in the replication of HCoV-229E. Elsevier B.V. 2008-02 2007-12-04 /pmc/articles/PMC7114149/ /pubmed/18055026 http://dx.doi.org/10.1016/j.antiviral.2007.10.011 Text en Copyright © 2007 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Kono, Masakazu
Tatsumi, Koichiro
Imai, Alberto M.
Saito, Kengo
Kuriyama, Takayuki
Shirasawa, Hiroshi
Inhibition of human coronavirus 229E infection in human epithelial lung cells (L132) by chloroquine: Involvement of p38 MAPK and ERK
title Inhibition of human coronavirus 229E infection in human epithelial lung cells (L132) by chloroquine: Involvement of p38 MAPK and ERK
title_full Inhibition of human coronavirus 229E infection in human epithelial lung cells (L132) by chloroquine: Involvement of p38 MAPK and ERK
title_fullStr Inhibition of human coronavirus 229E infection in human epithelial lung cells (L132) by chloroquine: Involvement of p38 MAPK and ERK
title_full_unstemmed Inhibition of human coronavirus 229E infection in human epithelial lung cells (L132) by chloroquine: Involvement of p38 MAPK and ERK
title_short Inhibition of human coronavirus 229E infection in human epithelial lung cells (L132) by chloroquine: Involvement of p38 MAPK and ERK
title_sort inhibition of human coronavirus 229e infection in human epithelial lung cells (l132) by chloroquine: involvement of p38 mapk and erk
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114149/
https://www.ncbi.nlm.nih.gov/pubmed/18055026
http://dx.doi.org/10.1016/j.antiviral.2007.10.011
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