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Activation of human monocytes after infection by human coronavirus 229E

Human coronaviruses (HCoV) are recognized respiratory pathogens that may be involved in other pathologies such as central nervous system (CNS) diseases. To investigate whether leukocytes could participate in respiratory pathologies and serve as vector for viral spread towards other tissues, the susc...

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Autores principales: Desforges, Marc, Miletti, Tina C., Gagnon, Mylène, Talbot, Pierre J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114174/
https://www.ncbi.nlm.nih.gov/pubmed/17669539
http://dx.doi.org/10.1016/j.virusres.2007.06.016
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author Desforges, Marc
Miletti, Tina C.
Gagnon, Mylène
Talbot, Pierre J.
author_facet Desforges, Marc
Miletti, Tina C.
Gagnon, Mylène
Talbot, Pierre J.
author_sort Desforges, Marc
collection PubMed
description Human coronaviruses (HCoV) are recognized respiratory pathogens that may be involved in other pathologies such as central nervous system (CNS) diseases. To investigate whether leukocytes could participate in respiratory pathologies and serve as vector for viral spread towards other tissues, the susceptibility of human leukocytic cell lines and peripheral blood mononuclear cells (PBMC) to HCoV-229E and HCoV-OC43 infection was investigated. Human primary monocytes/macrophages were susceptible to HCoV-229E infection, but strongly restricted HCoV-OC43 replication. Moreover, productive HCoV-229E infection of primary monocytes and of the THP-1 monocytic cell line led to their activation, as indicated by the production of pro-inflammatory mediators, including TNF-α, CCL5, CXCL10 and CXCL11 and MMP-9. Moreover, an in vitro chemotaxis assay showed that motility towards chemokines of THP-1 cells and primary monocytes was increased following an acute or persistent HCoV-229E infection. Taken together, these results suggest that infected monocytes could serve as a reservoir for HCoV-229E, become activated, participate in the exacerbation of pulmonary pathologies, as well as serve as potential vectors for viral dissemination to host tissues, where it could be associated with other pathologies.
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spelling pubmed-71141742020-04-02 Activation of human monocytes after infection by human coronavirus 229E Desforges, Marc Miletti, Tina C. Gagnon, Mylène Talbot, Pierre J. Virus Res Article Human coronaviruses (HCoV) are recognized respiratory pathogens that may be involved in other pathologies such as central nervous system (CNS) diseases. To investigate whether leukocytes could participate in respiratory pathologies and serve as vector for viral spread towards other tissues, the susceptibility of human leukocytic cell lines and peripheral blood mononuclear cells (PBMC) to HCoV-229E and HCoV-OC43 infection was investigated. Human primary monocytes/macrophages were susceptible to HCoV-229E infection, but strongly restricted HCoV-OC43 replication. Moreover, productive HCoV-229E infection of primary monocytes and of the THP-1 monocytic cell line led to their activation, as indicated by the production of pro-inflammatory mediators, including TNF-α, CCL5, CXCL10 and CXCL11 and MMP-9. Moreover, an in vitro chemotaxis assay showed that motility towards chemokines of THP-1 cells and primary monocytes was increased following an acute or persistent HCoV-229E infection. Taken together, these results suggest that infected monocytes could serve as a reservoir for HCoV-229E, become activated, participate in the exacerbation of pulmonary pathologies, as well as serve as potential vectors for viral dissemination to host tissues, where it could be associated with other pathologies. Elsevier B.V. 2007-12 2007-07-31 /pmc/articles/PMC7114174/ /pubmed/17669539 http://dx.doi.org/10.1016/j.virusres.2007.06.016 Text en Copyright © 2007 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Desforges, Marc
Miletti, Tina C.
Gagnon, Mylène
Talbot, Pierre J.
Activation of human monocytes after infection by human coronavirus 229E
title Activation of human monocytes after infection by human coronavirus 229E
title_full Activation of human monocytes after infection by human coronavirus 229E
title_fullStr Activation of human monocytes after infection by human coronavirus 229E
title_full_unstemmed Activation of human monocytes after infection by human coronavirus 229E
title_short Activation of human monocytes after infection by human coronavirus 229E
title_sort activation of human monocytes after infection by human coronavirus 229e
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114174/
https://www.ncbi.nlm.nih.gov/pubmed/17669539
http://dx.doi.org/10.1016/j.virusres.2007.06.016
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