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Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay
A small molecule library containing 480 known bioactive compounds was screened for antiviral activity against poliovirus (PV) using a cellular fluorescence resonance energy transfer (FRET) assay for viral protease activity. The infected reporter cells treated with the viral replication-suppressing c...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114228/ https://www.ncbi.nlm.nih.gov/pubmed/18243348 http://dx.doi.org/10.1016/j.antiviral.2007.12.009 |
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author | Hwang, Yu-Chen Chu, Justin Jang-Hann Yang, Priscilla L. Chen, Wilfred Yates, Marylynn V. |
author_facet | Hwang, Yu-Chen Chu, Justin Jang-Hann Yang, Priscilla L. Chen, Wilfred Yates, Marylynn V. |
author_sort | Hwang, Yu-Chen |
collection | PubMed |
description | A small molecule library containing 480 known bioactive compounds was screened for antiviral activity against poliovirus (PV) using a cellular fluorescence resonance energy transfer (FRET) assay for viral protease activity. The infected reporter cells treated with the viral replication-suppressing compounds were examined via fluorescence microscope 7.5 h postinfection. Twelve molecules showed moderate to potent antiviral activity at concentrations less than 32 μM during the primary screening. Three compounds, anisomycin, linoleic acid, and lycorine, were chosen for validation. A dose-dependent cytotoxicity assay and a secondary screening using conventional plaque assay were conducted to confirm the results. The developed method can be used for rapid screening for molecules with antiviral activity. |
format | Online Article Text |
id | pubmed-7114228 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71142282020-04-02 Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay Hwang, Yu-Chen Chu, Justin Jang-Hann Yang, Priscilla L. Chen, Wilfred Yates, Marylynn V. Antiviral Res Article A small molecule library containing 480 known bioactive compounds was screened for antiviral activity against poliovirus (PV) using a cellular fluorescence resonance energy transfer (FRET) assay for viral protease activity. The infected reporter cells treated with the viral replication-suppressing compounds were examined via fluorescence microscope 7.5 h postinfection. Twelve molecules showed moderate to potent antiviral activity at concentrations less than 32 μM during the primary screening. Three compounds, anisomycin, linoleic acid, and lycorine, were chosen for validation. A dose-dependent cytotoxicity assay and a secondary screening using conventional plaque assay were conducted to confirm the results. The developed method can be used for rapid screening for molecules with antiviral activity. Elsevier B.V. 2008-03 2008-01-16 /pmc/articles/PMC7114228/ /pubmed/18243348 http://dx.doi.org/10.1016/j.antiviral.2007.12.009 Text en Copyright © 2007 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Hwang, Yu-Chen Chu, Justin Jang-Hann Yang, Priscilla L. Chen, Wilfred Yates, Marylynn V. Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay |
title | Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay |
title_full | Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay |
title_fullStr | Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay |
title_full_unstemmed | Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay |
title_short | Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay |
title_sort | rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114228/ https://www.ncbi.nlm.nih.gov/pubmed/18243348 http://dx.doi.org/10.1016/j.antiviral.2007.12.009 |
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