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Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay

A small molecule library containing 480 known bioactive compounds was screened for antiviral activity against poliovirus (PV) using a cellular fluorescence resonance energy transfer (FRET) assay for viral protease activity. The infected reporter cells treated with the viral replication-suppressing c...

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Detalles Bibliográficos
Autores principales: Hwang, Yu-Chen, Chu, Justin Jang-Hann, Yang, Priscilla L., Chen, Wilfred, Yates, Marylynn V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114228/
https://www.ncbi.nlm.nih.gov/pubmed/18243348
http://dx.doi.org/10.1016/j.antiviral.2007.12.009
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author Hwang, Yu-Chen
Chu, Justin Jang-Hann
Yang, Priscilla L.
Chen, Wilfred
Yates, Marylynn V.
author_facet Hwang, Yu-Chen
Chu, Justin Jang-Hann
Yang, Priscilla L.
Chen, Wilfred
Yates, Marylynn V.
author_sort Hwang, Yu-Chen
collection PubMed
description A small molecule library containing 480 known bioactive compounds was screened for antiviral activity against poliovirus (PV) using a cellular fluorescence resonance energy transfer (FRET) assay for viral protease activity. The infected reporter cells treated with the viral replication-suppressing compounds were examined via fluorescence microscope 7.5 h postinfection. Twelve molecules showed moderate to potent antiviral activity at concentrations less than 32 μM during the primary screening. Three compounds, anisomycin, linoleic acid, and lycorine, were chosen for validation. A dose-dependent cytotoxicity assay and a secondary screening using conventional plaque assay were conducted to confirm the results. The developed method can be used for rapid screening for molecules with antiviral activity.
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spelling pubmed-71142282020-04-02 Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay Hwang, Yu-Chen Chu, Justin Jang-Hann Yang, Priscilla L. Chen, Wilfred Yates, Marylynn V. Antiviral Res Article A small molecule library containing 480 known bioactive compounds was screened for antiviral activity against poliovirus (PV) using a cellular fluorescence resonance energy transfer (FRET) assay for viral protease activity. The infected reporter cells treated with the viral replication-suppressing compounds were examined via fluorescence microscope 7.5 h postinfection. Twelve molecules showed moderate to potent antiviral activity at concentrations less than 32 μM during the primary screening. Three compounds, anisomycin, linoleic acid, and lycorine, were chosen for validation. A dose-dependent cytotoxicity assay and a secondary screening using conventional plaque assay were conducted to confirm the results. The developed method can be used for rapid screening for molecules with antiviral activity. Elsevier B.V. 2008-03 2008-01-16 /pmc/articles/PMC7114228/ /pubmed/18243348 http://dx.doi.org/10.1016/j.antiviral.2007.12.009 Text en Copyright © 2007 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Hwang, Yu-Chen
Chu, Justin Jang-Hann
Yang, Priscilla L.
Chen, Wilfred
Yates, Marylynn V.
Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay
title Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay
title_full Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay
title_fullStr Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay
title_full_unstemmed Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay
title_short Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay
title_sort rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114228/
https://www.ncbi.nlm.nih.gov/pubmed/18243348
http://dx.doi.org/10.1016/j.antiviral.2007.12.009
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