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Immune response induced by spike protein from transmissible gastroenteritis coronavirus expressed in mouse mammary cells

The present study is undertaken to investigate the immune response that was induced by the recombinant spike (S) protein from swine-transmissible gastroenteritis virus (TGEV) expressed in mouse mammary cells. A mammary-specific expression vector pEBS containing the full-length cDNA of S gene was con...

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Autores principales: Peng, Shu-Ying, Lv, Ning, Zhang, Yong, Bi, Cong-Ming, Cao, Jun-Wei, Li, Zhi-Yan, Zhang, Shi-Qiang, He, Xiao-Ning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114266/
https://www.ncbi.nlm.nih.gov/pubmed/17532081
http://dx.doi.org/10.1016/j.virusres.2007.04.004
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author Peng, Shu-Ying
Lv, Ning
Zhang, Yong
Bi, Cong-Ming
Cao, Jun-Wei
Li, Zhi-Yan
Zhang, Shi-Qiang
He, Xiao-Ning
author_facet Peng, Shu-Ying
Lv, Ning
Zhang, Yong
Bi, Cong-Ming
Cao, Jun-Wei
Li, Zhi-Yan
Zhang, Shi-Qiang
He, Xiao-Ning
author_sort Peng, Shu-Ying
collection PubMed
description The present study is undertaken to investigate the immune response that was induced by the recombinant spike (S) protein from swine-transmissible gastroenteritis virus (TGEV) expressed in mouse mammary cells. A mammary-specific expression vector pEBS containing the full-length cDNA of S gene was constructed and expressed in the mouse mammary cells (EMT6). The recombinant S protein from culture supernatant of transgenic EMT6 was harvested and immunized BALB/c mice. The results demonstrated recombinant S protein was expressed at high levels in mammary cells by Western blotting and enzyme-linked immunosorbent assay (ELISA) detection. The antibody titer in BALB/c mice following immunization with recombinant S protein was detectable after the first immunization. Maximum titers of antibody (8.86 ± 0.19 ng/ml of serum) were attained after the second immunization. In conclusion, the recombinant S protein expressed in mammary cells was able to elicit substantial immunological response against TGEV. This lays the basis for using mammary gland bioreactor generating edible vaccine.
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spelling pubmed-71142662020-04-02 Immune response induced by spike protein from transmissible gastroenteritis coronavirus expressed in mouse mammary cells Peng, Shu-Ying Lv, Ning Zhang, Yong Bi, Cong-Ming Cao, Jun-Wei Li, Zhi-Yan Zhang, Shi-Qiang He, Xiao-Ning Virus Res Article The present study is undertaken to investigate the immune response that was induced by the recombinant spike (S) protein from swine-transmissible gastroenteritis virus (TGEV) expressed in mouse mammary cells. A mammary-specific expression vector pEBS containing the full-length cDNA of S gene was constructed and expressed in the mouse mammary cells (EMT6). The recombinant S protein from culture supernatant of transgenic EMT6 was harvested and immunized BALB/c mice. The results demonstrated recombinant S protein was expressed at high levels in mammary cells by Western blotting and enzyme-linked immunosorbent assay (ELISA) detection. The antibody titer in BALB/c mice following immunization with recombinant S protein was detectable after the first immunization. Maximum titers of antibody (8.86 ± 0.19 ng/ml of serum) were attained after the second immunization. In conclusion, the recombinant S protein expressed in mammary cells was able to elicit substantial immunological response against TGEV. This lays the basis for using mammary gland bioreactor generating edible vaccine. Published by Elsevier B.V. 2007-09 2007-05-25 /pmc/articles/PMC7114266/ /pubmed/17532081 http://dx.doi.org/10.1016/j.virusres.2007.04.004 Text en Copyright © 2007 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Peng, Shu-Ying
Lv, Ning
Zhang, Yong
Bi, Cong-Ming
Cao, Jun-Wei
Li, Zhi-Yan
Zhang, Shi-Qiang
He, Xiao-Ning
Immune response induced by spike protein from transmissible gastroenteritis coronavirus expressed in mouse mammary cells
title Immune response induced by spike protein from transmissible gastroenteritis coronavirus expressed in mouse mammary cells
title_full Immune response induced by spike protein from transmissible gastroenteritis coronavirus expressed in mouse mammary cells
title_fullStr Immune response induced by spike protein from transmissible gastroenteritis coronavirus expressed in mouse mammary cells
title_full_unstemmed Immune response induced by spike protein from transmissible gastroenteritis coronavirus expressed in mouse mammary cells
title_short Immune response induced by spike protein from transmissible gastroenteritis coronavirus expressed in mouse mammary cells
title_sort immune response induced by spike protein from transmissible gastroenteritis coronavirus expressed in mouse mammary cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114266/
https://www.ncbi.nlm.nih.gov/pubmed/17532081
http://dx.doi.org/10.1016/j.virusres.2007.04.004
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