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In vitro inhibition of CSFV replication by multiple siRNA expression
Classical swine fever (CSF) is a highly contagious viral disease of pigs which causes major economic losses worldwide. No specific drug is currently available for the effective treatment of CSFV infection; however, RNA interference (RNAi) has been applied successfully to inhibit the replication of h...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114328/ https://www.ncbi.nlm.nih.gov/pubmed/21699919 http://dx.doi.org/10.1016/j.antiviral.2011.06.005 |
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author | Li, Jiangnan Dai, Yajuan Liu, Shuai Guo, Huancheng Wang, Tiedong Ouyang, Hongsheng Tu, Changchun |
author_facet | Li, Jiangnan Dai, Yajuan Liu, Shuai Guo, Huancheng Wang, Tiedong Ouyang, Hongsheng Tu, Changchun |
author_sort | Li, Jiangnan |
collection | PubMed |
description | Classical swine fever (CSF) is a highly contagious viral disease of pigs which causes major economic losses worldwide. No specific drug is currently available for the effective treatment of CSFV infection; however, RNA interference (RNAi) has been applied successfully to inhibit the replication of human and other animal viruses. In this study, three effective siRNAs targeting NS3 of CSFV were selected. siNS3-2 targeting NS3 gene was chosen for further experimentation, while siN1 and siN2 targeting N(pro) gene, and siNS5B targeting NS5B gene describe previously. Single, double and quadruple anti-CSFV siRNA expression plasmids, with loxp sites at each end of the selectable marker genes, were constructed and analyzed using the same promoters or four different promoters, targeting N(pro), NS3 and NS5B genes of CSFV. Results indicate that single or multiple siRNA expression plasmids can efficiently inhibit CSFV replication and that inhibition was markedly stronger when multiple siRNAs were expressed targeting different genes of CSFV. Since RNAi applied to anti-CSFV research, this study provides anti-CSFV methods by single and multiple siRNA expression which can target most viral isolates of different subtypes and prevent viral escape. It also provides a basis for development of CSFV-resistant transgenic pigs. |
format | Online Article Text |
id | pubmed-7114328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71143282020-04-02 In vitro inhibition of CSFV replication by multiple siRNA expression Li, Jiangnan Dai, Yajuan Liu, Shuai Guo, Huancheng Wang, Tiedong Ouyang, Hongsheng Tu, Changchun Antiviral Res Article Classical swine fever (CSF) is a highly contagious viral disease of pigs which causes major economic losses worldwide. No specific drug is currently available for the effective treatment of CSFV infection; however, RNA interference (RNAi) has been applied successfully to inhibit the replication of human and other animal viruses. In this study, three effective siRNAs targeting NS3 of CSFV were selected. siNS3-2 targeting NS3 gene was chosen for further experimentation, while siN1 and siN2 targeting N(pro) gene, and siNS5B targeting NS5B gene describe previously. Single, double and quadruple anti-CSFV siRNA expression plasmids, with loxp sites at each end of the selectable marker genes, were constructed and analyzed using the same promoters or four different promoters, targeting N(pro), NS3 and NS5B genes of CSFV. Results indicate that single or multiple siRNA expression plasmids can efficiently inhibit CSFV replication and that inhibition was markedly stronger when multiple siRNAs were expressed targeting different genes of CSFV. Since RNAi applied to anti-CSFV research, this study provides anti-CSFV methods by single and multiple siRNA expression which can target most viral isolates of different subtypes and prevent viral escape. It also provides a basis for development of CSFV-resistant transgenic pigs. Elsevier B.V. 2011-08 2011-06-14 /pmc/articles/PMC7114328/ /pubmed/21699919 http://dx.doi.org/10.1016/j.antiviral.2011.06.005 Text en Copyright © 2011 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Li, Jiangnan Dai, Yajuan Liu, Shuai Guo, Huancheng Wang, Tiedong Ouyang, Hongsheng Tu, Changchun In vitro inhibition of CSFV replication by multiple siRNA expression |
title | In vitro inhibition of CSFV replication by multiple siRNA expression |
title_full | In vitro inhibition of CSFV replication by multiple siRNA expression |
title_fullStr | In vitro inhibition of CSFV replication by multiple siRNA expression |
title_full_unstemmed | In vitro inhibition of CSFV replication by multiple siRNA expression |
title_short | In vitro inhibition of CSFV replication by multiple siRNA expression |
title_sort | in vitro inhibition of csfv replication by multiple sirna expression |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114328/ https://www.ncbi.nlm.nih.gov/pubmed/21699919 http://dx.doi.org/10.1016/j.antiviral.2011.06.005 |
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