Cargando…
Peptide nucleic acids: Advanced tools for biomedical applications
Peptide Nucleic Acids (PNAs) are the DNA/RNA analogues in which sugar-phosphate backbone is replaced by N-2-aminoethylglycine repeating units. PNA contains neutral backbone hence due to the absence of electrostatic repulsion, its hybridization shows remarkable stability towards complementary oligonu...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114329/ https://www.ncbi.nlm.nih.gov/pubmed/28764969 http://dx.doi.org/10.1016/j.jbiotec.2017.07.026 |
_version_ | 1783513862465650688 |
---|---|
author | Gupta, Anjali Mishra, Anuradha Puri, Nidhi |
author_facet | Gupta, Anjali Mishra, Anuradha Puri, Nidhi |
author_sort | Gupta, Anjali |
collection | PubMed |
description | Peptide Nucleic Acids (PNAs) are the DNA/RNA analogues in which sugar-phosphate backbone is replaced by N-2-aminoethylglycine repeating units. PNA contains neutral backbone hence due to the absence of electrostatic repulsion, its hybridization shows remarkable stability towards complementary oligonucleotides. PNAs are highly resistant to cleavage by chemicals and enzymes due to the substrate specific nature of enzymes and therefore not degraded inside the cells. PNAs are emerging as new tools in the market due to their applications in antisense and antigene therapies by inhibiting translation and transcription respectively. Hence, several methods based on PNAs have been developed for designing various anticancer and antigene drugs, detection of mutations or modulation of PCR reactions. The duplex homopurine sequence of DNA may also be recognized by PNA, forming firm PNA/DNA/PNA triplex through strand invasion with a looped-out DNA strand. PNAs have also been found to replace DNA probes in varied investigative purposes. There are several disadvantages regarding cellular uptake of PNA, so modifications in PNA backbone or covalent coupling with cell penetrating peptides is necessary to improve its delivery inside the cells. In this review, hybridization properties along with potential applications of PNA in the field of diagnostics and pharmaceuticals are elaborated. |
format | Online Article Text |
id | pubmed-7114329 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71143292020-04-02 Peptide nucleic acids: Advanced tools for biomedical applications Gupta, Anjali Mishra, Anuradha Puri, Nidhi J Biotechnol Review Peptide Nucleic Acids (PNAs) are the DNA/RNA analogues in which sugar-phosphate backbone is replaced by N-2-aminoethylglycine repeating units. PNA contains neutral backbone hence due to the absence of electrostatic repulsion, its hybridization shows remarkable stability towards complementary oligonucleotides. PNAs are highly resistant to cleavage by chemicals and enzymes due to the substrate specific nature of enzymes and therefore not degraded inside the cells. PNAs are emerging as new tools in the market due to their applications in antisense and antigene therapies by inhibiting translation and transcription respectively. Hence, several methods based on PNAs have been developed for designing various anticancer and antigene drugs, detection of mutations or modulation of PCR reactions. The duplex homopurine sequence of DNA may also be recognized by PNA, forming firm PNA/DNA/PNA triplex through strand invasion with a looped-out DNA strand. PNAs have also been found to replace DNA probes in varied investigative purposes. There are several disadvantages regarding cellular uptake of PNA, so modifications in PNA backbone or covalent coupling with cell penetrating peptides is necessary to improve its delivery inside the cells. In this review, hybridization properties along with potential applications of PNA in the field of diagnostics and pharmaceuticals are elaborated. Elsevier B.V. 2017-10-10 2017-07-29 /pmc/articles/PMC7114329/ /pubmed/28764969 http://dx.doi.org/10.1016/j.jbiotec.2017.07.026 Text en © 2017 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Review Gupta, Anjali Mishra, Anuradha Puri, Nidhi Peptide nucleic acids: Advanced tools for biomedical applications |
title | Peptide nucleic acids: Advanced tools for biomedical applications |
title_full | Peptide nucleic acids: Advanced tools for biomedical applications |
title_fullStr | Peptide nucleic acids: Advanced tools for biomedical applications |
title_full_unstemmed | Peptide nucleic acids: Advanced tools for biomedical applications |
title_short | Peptide nucleic acids: Advanced tools for biomedical applications |
title_sort | peptide nucleic acids: advanced tools for biomedical applications |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114329/ https://www.ncbi.nlm.nih.gov/pubmed/28764969 http://dx.doi.org/10.1016/j.jbiotec.2017.07.026 |
work_keys_str_mv | AT guptaanjali peptidenucleicacidsadvancedtoolsforbiomedicalapplications AT mishraanuradha peptidenucleicacidsadvancedtoolsforbiomedicalapplications AT purinidhi peptidenucleicacidsadvancedtoolsforbiomedicalapplications |