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Distribution of a novel binding site for angiotensins II and III in mouse tissues

A novel binding site for angiotensins II and III that is unmasked by parachloromercuribenzoate has been reported in rat, mouse and human brains. Initial studies of this binding site indicate that it is not expressed in the adrenal, liver or kidney of the rat and mouse. To determine if this binding s...

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Autores principales: Rabey, Felicia M., Karamyan, Vardan T., Speth, Robert C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114337/
https://www.ncbi.nlm.nih.gov/pubmed/20171994
http://dx.doi.org/10.1016/j.regpep.2010.02.007
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author Rabey, Felicia M.
Karamyan, Vardan T.
Speth, Robert C.
author_facet Rabey, Felicia M.
Karamyan, Vardan T.
Speth, Robert C.
author_sort Rabey, Felicia M.
collection PubMed
description A novel binding site for angiotensins II and III that is unmasked by parachloromercuribenzoate has been reported in rat, mouse and human brains. Initial studies of this binding site indicate that it is not expressed in the adrenal, liver or kidney of the rat and mouse. To determine if this binding site occurs in other mouse tissues, 8 tissues were assayed for expression of this binding site by radioligand binding assay and compared with the expression of this binding site in the forebrain. Particulate fractions of homogenates of testis, epididymis, seminal vesicles, heart, spleen, pancreas, lung, skeletal muscle, and forebrain were incubated with (125)I-sarcosine(1), isoleucine(8) angiotensin II in the presence or absence of 0.3 mM parachloromercuribenzoate plus 10 µM losartan and 10 µM PD123319 (to saturate AT(1) and AT(2) receptors). Specific (3 µM angiotensin II displaceable) high affinity binding occurred in the testis > forebrain > epididymis > spleen > pancreas > lung when parachloromercuribenzoate was present. Binding could not be reliably observed in heart, skeletal muscle and seminal vesicles. High affinity binding of (125)I-sarcosine(1), isoleucine(8) angiotensin II was observed in the absence of parachloromercuribenzoate in the pancreas on occasion. This suggests that this novel angiotensin binding site may have a functional role in these tissues.
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spelling pubmed-71143372020-04-02 Distribution of a novel binding site for angiotensins II and III in mouse tissues Rabey, Felicia M. Karamyan, Vardan T. Speth, Robert C. Regul Pept Short Communication A novel binding site for angiotensins II and III that is unmasked by parachloromercuribenzoate has been reported in rat, mouse and human brains. Initial studies of this binding site indicate that it is not expressed in the adrenal, liver or kidney of the rat and mouse. To determine if this binding site occurs in other mouse tissues, 8 tissues were assayed for expression of this binding site by radioligand binding assay and compared with the expression of this binding site in the forebrain. Particulate fractions of homogenates of testis, epididymis, seminal vesicles, heart, spleen, pancreas, lung, skeletal muscle, and forebrain were incubated with (125)I-sarcosine(1), isoleucine(8) angiotensin II in the presence or absence of 0.3 mM parachloromercuribenzoate plus 10 µM losartan and 10 µM PD123319 (to saturate AT(1) and AT(2) receptors). Specific (3 µM angiotensin II displaceable) high affinity binding occurred in the testis > forebrain > epididymis > spleen > pancreas > lung when parachloromercuribenzoate was present. Binding could not be reliably observed in heart, skeletal muscle and seminal vesicles. High affinity binding of (125)I-sarcosine(1), isoleucine(8) angiotensin II was observed in the absence of parachloromercuribenzoate in the pancreas on occasion. This suggests that this novel angiotensin binding site may have a functional role in these tissues. Elsevier B.V. 2010-06-08 2010-02-19 /pmc/articles/PMC7114337/ /pubmed/20171994 http://dx.doi.org/10.1016/j.regpep.2010.02.007 Text en Copyright © 2010 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Short Communication
Rabey, Felicia M.
Karamyan, Vardan T.
Speth, Robert C.
Distribution of a novel binding site for angiotensins II and III in mouse tissues
title Distribution of a novel binding site for angiotensins II and III in mouse tissues
title_full Distribution of a novel binding site for angiotensins II and III in mouse tissues
title_fullStr Distribution of a novel binding site for angiotensins II and III in mouse tissues
title_full_unstemmed Distribution of a novel binding site for angiotensins II and III in mouse tissues
title_short Distribution of a novel binding site for angiotensins II and III in mouse tissues
title_sort distribution of a novel binding site for angiotensins ii and iii in mouse tissues
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114337/
https://www.ncbi.nlm.nih.gov/pubmed/20171994
http://dx.doi.org/10.1016/j.regpep.2010.02.007
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