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Development of transgenic mouse model expressing porcine aminopeptidase N and its susceptibility to porcine epidemic diarrhea virus

Porcine coronavirus infections have known as they are specific to pigs with predominantly enteric or respiratory diseases. No laboratory animal model is yet been developed in porcine coronaviruses study. Here, we report that development of a transgenic mouse model expressing porcine APN which is sus...

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Autores principales: Park, Jung-Eun, Park, Eui-Soon, Yu, Jung-Eun, Rho, Jaerang, Paudel, Sarita, Hyun, Bang-Hun, Yang, Dong-Kun, Shin, Hyun-Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114392/
https://www.ncbi.nlm.nih.gov/pubmed/25550073
http://dx.doi.org/10.1016/j.virusres.2014.12.024
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author Park, Jung-Eun
Park, Eui-Soon
Yu, Jung-Eun
Rho, Jaerang
Paudel, Sarita
Hyun, Bang-Hun
Yang, Dong-Kun
Shin, Hyun-Jin
author_facet Park, Jung-Eun
Park, Eui-Soon
Yu, Jung-Eun
Rho, Jaerang
Paudel, Sarita
Hyun, Bang-Hun
Yang, Dong-Kun
Shin, Hyun-Jin
author_sort Park, Jung-Eun
collection PubMed
description Porcine coronavirus infections have known as they are specific to pigs with predominantly enteric or respiratory diseases. No laboratory animal model is yet been developed in porcine coronaviruses study. Here, we report that development of a transgenic mouse model expressing porcine APN which is susceptible to porcine coronavirus infection. The porcine APN transgene was constructed by fusing with mouse proximal APN promoter at 5′ terminus and bovine growth hormone polyadenylation site at its 3′ terminus. After screen on pubs from the microinjected mice, we confirmed two transgenic lines expressing porcine APN in various organs. We confirmed the susceptibility to porcine epidemic diarrhea virus, one of the porcine coronaviruses. These transgenic mice will be an important tool for research into the porcine coronaviruses.
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spelling pubmed-71143922020-04-02 Development of transgenic mouse model expressing porcine aminopeptidase N and its susceptibility to porcine epidemic diarrhea virus Park, Jung-Eun Park, Eui-Soon Yu, Jung-Eun Rho, Jaerang Paudel, Sarita Hyun, Bang-Hun Yang, Dong-Kun Shin, Hyun-Jin Virus Res Article Porcine coronavirus infections have known as they are specific to pigs with predominantly enteric or respiratory diseases. No laboratory animal model is yet been developed in porcine coronaviruses study. Here, we report that development of a transgenic mouse model expressing porcine APN which is susceptible to porcine coronavirus infection. The porcine APN transgene was constructed by fusing with mouse proximal APN promoter at 5′ terminus and bovine growth hormone polyadenylation site at its 3′ terminus. After screen on pubs from the microinjected mice, we confirmed two transgenic lines expressing porcine APN in various organs. We confirmed the susceptibility to porcine epidemic diarrhea virus, one of the porcine coronaviruses. These transgenic mice will be an important tool for research into the porcine coronaviruses. Elsevier B.V. 2015-02-02 2014-12-27 /pmc/articles/PMC7114392/ /pubmed/25550073 http://dx.doi.org/10.1016/j.virusres.2014.12.024 Text en Copyright © 2014 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Park, Jung-Eun
Park, Eui-Soon
Yu, Jung-Eun
Rho, Jaerang
Paudel, Sarita
Hyun, Bang-Hun
Yang, Dong-Kun
Shin, Hyun-Jin
Development of transgenic mouse model expressing porcine aminopeptidase N and its susceptibility to porcine epidemic diarrhea virus
title Development of transgenic mouse model expressing porcine aminopeptidase N and its susceptibility to porcine epidemic diarrhea virus
title_full Development of transgenic mouse model expressing porcine aminopeptidase N and its susceptibility to porcine epidemic diarrhea virus
title_fullStr Development of transgenic mouse model expressing porcine aminopeptidase N and its susceptibility to porcine epidemic diarrhea virus
title_full_unstemmed Development of transgenic mouse model expressing porcine aminopeptidase N and its susceptibility to porcine epidemic diarrhea virus
title_short Development of transgenic mouse model expressing porcine aminopeptidase N and its susceptibility to porcine epidemic diarrhea virus
title_sort development of transgenic mouse model expressing porcine aminopeptidase n and its susceptibility to porcine epidemic diarrhea virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114392/
https://www.ncbi.nlm.nih.gov/pubmed/25550073
http://dx.doi.org/10.1016/j.virusres.2014.12.024
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