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Development of transgenic mouse model expressing porcine aminopeptidase N and its susceptibility to porcine epidemic diarrhea virus
Porcine coronavirus infections have known as they are specific to pigs with predominantly enteric or respiratory diseases. No laboratory animal model is yet been developed in porcine coronaviruses study. Here, we report that development of a transgenic mouse model expressing porcine APN which is sus...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114392/ https://www.ncbi.nlm.nih.gov/pubmed/25550073 http://dx.doi.org/10.1016/j.virusres.2014.12.024 |
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author | Park, Jung-Eun Park, Eui-Soon Yu, Jung-Eun Rho, Jaerang Paudel, Sarita Hyun, Bang-Hun Yang, Dong-Kun Shin, Hyun-Jin |
author_facet | Park, Jung-Eun Park, Eui-Soon Yu, Jung-Eun Rho, Jaerang Paudel, Sarita Hyun, Bang-Hun Yang, Dong-Kun Shin, Hyun-Jin |
author_sort | Park, Jung-Eun |
collection | PubMed |
description | Porcine coronavirus infections have known as they are specific to pigs with predominantly enteric or respiratory diseases. No laboratory animal model is yet been developed in porcine coronaviruses study. Here, we report that development of a transgenic mouse model expressing porcine APN which is susceptible to porcine coronavirus infection. The porcine APN transgene was constructed by fusing with mouse proximal APN promoter at 5′ terminus and bovine growth hormone polyadenylation site at its 3′ terminus. After screen on pubs from the microinjected mice, we confirmed two transgenic lines expressing porcine APN in various organs. We confirmed the susceptibility to porcine epidemic diarrhea virus, one of the porcine coronaviruses. These transgenic mice will be an important tool for research into the porcine coronaviruses. |
format | Online Article Text |
id | pubmed-7114392 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71143922020-04-02 Development of transgenic mouse model expressing porcine aminopeptidase N and its susceptibility to porcine epidemic diarrhea virus Park, Jung-Eun Park, Eui-Soon Yu, Jung-Eun Rho, Jaerang Paudel, Sarita Hyun, Bang-Hun Yang, Dong-Kun Shin, Hyun-Jin Virus Res Article Porcine coronavirus infections have known as they are specific to pigs with predominantly enteric or respiratory diseases. No laboratory animal model is yet been developed in porcine coronaviruses study. Here, we report that development of a transgenic mouse model expressing porcine APN which is susceptible to porcine coronavirus infection. The porcine APN transgene was constructed by fusing with mouse proximal APN promoter at 5′ terminus and bovine growth hormone polyadenylation site at its 3′ terminus. After screen on pubs from the microinjected mice, we confirmed two transgenic lines expressing porcine APN in various organs. We confirmed the susceptibility to porcine epidemic diarrhea virus, one of the porcine coronaviruses. These transgenic mice will be an important tool for research into the porcine coronaviruses. Elsevier B.V. 2015-02-02 2014-12-27 /pmc/articles/PMC7114392/ /pubmed/25550073 http://dx.doi.org/10.1016/j.virusres.2014.12.024 Text en Copyright © 2014 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Park, Jung-Eun Park, Eui-Soon Yu, Jung-Eun Rho, Jaerang Paudel, Sarita Hyun, Bang-Hun Yang, Dong-Kun Shin, Hyun-Jin Development of transgenic mouse model expressing porcine aminopeptidase N and its susceptibility to porcine epidemic diarrhea virus |
title | Development of transgenic mouse model expressing porcine aminopeptidase N and its susceptibility to porcine epidemic diarrhea virus |
title_full | Development of transgenic mouse model expressing porcine aminopeptidase N and its susceptibility to porcine epidemic diarrhea virus |
title_fullStr | Development of transgenic mouse model expressing porcine aminopeptidase N and its susceptibility to porcine epidemic diarrhea virus |
title_full_unstemmed | Development of transgenic mouse model expressing porcine aminopeptidase N and its susceptibility to porcine epidemic diarrhea virus |
title_short | Development of transgenic mouse model expressing porcine aminopeptidase N and its susceptibility to porcine epidemic diarrhea virus |
title_sort | development of transgenic mouse model expressing porcine aminopeptidase n and its susceptibility to porcine epidemic diarrhea virus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114392/ https://www.ncbi.nlm.nih.gov/pubmed/25550073 http://dx.doi.org/10.1016/j.virusres.2014.12.024 |
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