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Mutations in the coat protein-binding cis-acting RNA motifs debilitate RNA recombination of Brome mosaic virus
We have previously described the efficient homologous recombination system between 5′ subgenomic RNA3a (sgRNA3a) and genomic RNA3 of Brome mosaic virus (BMV) in barley protoplasts (Sztuba-Solińska et al., 2011a). Here, we demonstrated that sequence alterations in the coat protein (CP)-binding cis-ac...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114393/ https://www.ncbi.nlm.nih.gov/pubmed/23079110 http://dx.doi.org/10.1016/j.virusres.2012.10.001 |
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author | Sztuba-Solińska, Joanna Fanning, Sean W. Horn, James R. Bujarski, Jozef J. |
author_facet | Sztuba-Solińska, Joanna Fanning, Sean W. Horn, James R. Bujarski, Jozef J. |
author_sort | Sztuba-Solińska, Joanna |
collection | PubMed |
description | We have previously described the efficient homologous recombination system between 5′ subgenomic RNA3a (sgRNA3a) and genomic RNA3 of Brome mosaic virus (BMV) in barley protoplasts (Sztuba-Solińska et al., 2011a). Here, we demonstrated that sequence alterations in the coat protein (CP)-binding cis-acting RNA motifs, the Bbox region (in the intercistronic RNA3 sequence) and the RNA3 packaging element (PE, in the movement protein ORF), reduced crossover frequencies in protoplasts. Additionally, the modification of Bbox-like element in the 5′ UTR region strongly debilitated crossovers. Along the lines of these observations, RNA3 mutants not expressing CP or expressing mutated CPs also reduced recombination. A series of reciprocal transfections demonstrated a functional crosstalk between the Bbox and PE elements. Altogether, our data imply the role of CP in sgRNA3a-directed recombination by either facilitating the interaction of the RNA substrates and/or by creating roadblocks for the viral replicase. |
format | Online Article Text |
id | pubmed-7114393 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71143932020-04-02 Mutations in the coat protein-binding cis-acting RNA motifs debilitate RNA recombination of Brome mosaic virus Sztuba-Solińska, Joanna Fanning, Sean W. Horn, James R. Bujarski, Jozef J. Virus Res Article We have previously described the efficient homologous recombination system between 5′ subgenomic RNA3a (sgRNA3a) and genomic RNA3 of Brome mosaic virus (BMV) in barley protoplasts (Sztuba-Solińska et al., 2011a). Here, we demonstrated that sequence alterations in the coat protein (CP)-binding cis-acting RNA motifs, the Bbox region (in the intercistronic RNA3 sequence) and the RNA3 packaging element (PE, in the movement protein ORF), reduced crossover frequencies in protoplasts. Additionally, the modification of Bbox-like element in the 5′ UTR region strongly debilitated crossovers. Along the lines of these observations, RNA3 mutants not expressing CP or expressing mutated CPs also reduced recombination. A series of reciprocal transfections demonstrated a functional crosstalk between the Bbox and PE elements. Altogether, our data imply the role of CP in sgRNA3a-directed recombination by either facilitating the interaction of the RNA substrates and/or by creating roadblocks for the viral replicase. Elsevier B.V. 2012-12 2012-10-16 /pmc/articles/PMC7114393/ /pubmed/23079110 http://dx.doi.org/10.1016/j.virusres.2012.10.001 Text en Copyright © 2012 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Sztuba-Solińska, Joanna Fanning, Sean W. Horn, James R. Bujarski, Jozef J. Mutations in the coat protein-binding cis-acting RNA motifs debilitate RNA recombination of Brome mosaic virus |
title | Mutations in the coat protein-binding cis-acting RNA motifs debilitate RNA recombination of Brome mosaic virus |
title_full | Mutations in the coat protein-binding cis-acting RNA motifs debilitate RNA recombination of Brome mosaic virus |
title_fullStr | Mutations in the coat protein-binding cis-acting RNA motifs debilitate RNA recombination of Brome mosaic virus |
title_full_unstemmed | Mutations in the coat protein-binding cis-acting RNA motifs debilitate RNA recombination of Brome mosaic virus |
title_short | Mutations in the coat protein-binding cis-acting RNA motifs debilitate RNA recombination of Brome mosaic virus |
title_sort | mutations in the coat protein-binding cis-acting rna motifs debilitate rna recombination of brome mosaic virus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114393/ https://www.ncbi.nlm.nih.gov/pubmed/23079110 http://dx.doi.org/10.1016/j.virusres.2012.10.001 |
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