Identification of a linear epitope on the capsid protein of classical swine fever virus

The capsid (C) protein of Classical swine fever virus (CSFV) is proposed to play an essential role in the replication and translation of the viral RNA. In this study, a monoclonal antibody (mAb) directed against the C protein was generated with the recombinant C protein expressed in Escherichia coli...

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Detalles Bibliográficos
Autores principales: Zhang, Xin, Xu, Jia, Sun, Yuan, Li, Su, Li, Na, Yang, Shuo, He, Fan, Huang, Jun-Hua, Ling, Li-Jun, Qiu, Hua-Ji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114404/
https://www.ncbi.nlm.nih.gov/pubmed/21255622
http://dx.doi.org/10.1016/j.virusres.2011.01.009
Descripción
Sumario:The capsid (C) protein of Classical swine fever virus (CSFV) is proposed to play an essential role in the replication and translation of the viral RNA. In this study, a monoclonal antibody (mAb) directed against the C protein was generated with the recombinant C protein expressed in Escherichia coli as immunogen. IFA and IPMA analysis showed that the native C protein of CSFV virions was reactive to the mAb. By truncating the C protein, we identified a linear epitope recognized by the mAb, corresponding to amino acids (61)TQDGLYHNKN(70) of the CSFV C protein, which is well conserved among pestiviruses. Laser confocal analysis showed that the C protein mainly locates in the cellular nucleoplasm and nucleolus of PK-15 cells. The results have implications for further study of CSFV replication.

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