Identification of a linear epitope on the capsid protein of classical swine fever virus

The capsid (C) protein of Classical swine fever virus (CSFV) is proposed to play an essential role in the replication and translation of the viral RNA. In this study, a monoclonal antibody (mAb) directed against the C protein was generated with the recombinant C protein expressed in Escherichia coli...

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Autores principales: Zhang, Xin, Xu, Jia, Sun, Yuan, Li, Su, Li, Na, Yang, Shuo, He, Fan, Huang, Jun-Hua, Ling, Li-Jun, Qiu, Hua-Ji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114404/
https://www.ncbi.nlm.nih.gov/pubmed/21255622
http://dx.doi.org/10.1016/j.virusres.2011.01.009
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author Zhang, Xin
Xu, Jia
Sun, Yuan
Li, Su
Li, Na
Yang, Shuo
He, Fan
Huang, Jun-Hua
Ling, Li-Jun
Qiu, Hua-Ji
author_facet Zhang, Xin
Xu, Jia
Sun, Yuan
Li, Su
Li, Na
Yang, Shuo
He, Fan
Huang, Jun-Hua
Ling, Li-Jun
Qiu, Hua-Ji
author_sort Zhang, Xin
collection PubMed
description The capsid (C) protein of Classical swine fever virus (CSFV) is proposed to play an essential role in the replication and translation of the viral RNA. In this study, a monoclonal antibody (mAb) directed against the C protein was generated with the recombinant C protein expressed in Escherichia coli as immunogen. IFA and IPMA analysis showed that the native C protein of CSFV virions was reactive to the mAb. By truncating the C protein, we identified a linear epitope recognized by the mAb, corresponding to amino acids (61)TQDGLYHNKN(70) of the CSFV C protein, which is well conserved among pestiviruses. Laser confocal analysis showed that the C protein mainly locates in the cellular nucleoplasm and nucleolus of PK-15 cells. The results have implications for further study of CSFV replication.
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spelling pubmed-71144042020-04-02 Identification of a linear epitope on the capsid protein of classical swine fever virus Zhang, Xin Xu, Jia Sun, Yuan Li, Su Li, Na Yang, Shuo He, Fan Huang, Jun-Hua Ling, Li-Jun Qiu, Hua-Ji Virus Res Article The capsid (C) protein of Classical swine fever virus (CSFV) is proposed to play an essential role in the replication and translation of the viral RNA. In this study, a monoclonal antibody (mAb) directed against the C protein was generated with the recombinant C protein expressed in Escherichia coli as immunogen. IFA and IPMA analysis showed that the native C protein of CSFV virions was reactive to the mAb. By truncating the C protein, we identified a linear epitope recognized by the mAb, corresponding to amino acids (61)TQDGLYHNKN(70) of the CSFV C protein, which is well conserved among pestiviruses. Laser confocal analysis showed that the C protein mainly locates in the cellular nucleoplasm and nucleolus of PK-15 cells. The results have implications for further study of CSFV replication. Elsevier B.V. 2011-03 2011-01-19 /pmc/articles/PMC7114404/ /pubmed/21255622 http://dx.doi.org/10.1016/j.virusres.2011.01.009 Text en Copyright © 2011 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Zhang, Xin
Xu, Jia
Sun, Yuan
Li, Su
Li, Na
Yang, Shuo
He, Fan
Huang, Jun-Hua
Ling, Li-Jun
Qiu, Hua-Ji
Identification of a linear epitope on the capsid protein of classical swine fever virus
title Identification of a linear epitope on the capsid protein of classical swine fever virus
title_full Identification of a linear epitope on the capsid protein of classical swine fever virus
title_fullStr Identification of a linear epitope on the capsid protein of classical swine fever virus
title_full_unstemmed Identification of a linear epitope on the capsid protein of classical swine fever virus
title_short Identification of a linear epitope on the capsid protein of classical swine fever virus
title_sort identification of a linear epitope on the capsid protein of classical swine fever virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114404/
https://www.ncbi.nlm.nih.gov/pubmed/21255622
http://dx.doi.org/10.1016/j.virusres.2011.01.009
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