Cargando…

Clathrin- and serine proteases-dependent uptake of porcine epidemic diarrhea virus into Vero cells

Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus, is a causative agent of porcine enteric disease characterized by acute watery diarrhea and dehydration in sucking piglet. Similar to other coronaviruses, PEDV spike protein mediates its cell entry by binding to cellular...

Descripción completa

Detalles Bibliográficos
Autores principales: Park, Jung-Eun, Cruz, Deu John M., Shin, Hyun-Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114442/
https://www.ncbi.nlm.nih.gov/pubmed/25086180
http://dx.doi.org/10.1016/j.virusres.2014.07.022
_version_ 1783513887111380992
author Park, Jung-Eun
Cruz, Deu John M.
Shin, Hyun-Jin
author_facet Park, Jung-Eun
Cruz, Deu John M.
Shin, Hyun-Jin
author_sort Park, Jung-Eun
collection PubMed
description Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus, is a causative agent of porcine enteric disease characterized by acute watery diarrhea and dehydration in sucking piglet. Similar to other coronaviruses, PEDV spike protein mediates its cell entry by binding to cellular receptors and inducing membrane fusion between viral envelopes and cellular membranes. However, the entry mechanism of PEDV is not studied. Here, we determined the entry mechanism of PEDV into Vero cells. Our data confirmed that PEDV entry followed clathrin-mediated endocytosis independence of caveolae-coated pit assembly. The internalized PEDV was co-localized with the clathrin-mediated endocytic marker, but not with the caveolae-mediated endocytic marker. In addition, cells treated with lysosomotropic agents and serine protease inhibitors were resistant to PEDV. Our data revealed that PEDV entry followed clathrin-mediated endocytosis and was dependent on a low pH and serine proteolysis for successful entry into cells.
format Online
Article
Text
id pubmed-7114442
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Elsevier B.V.
record_format MEDLINE/PubMed
spelling pubmed-71144422020-04-02 Clathrin- and serine proteases-dependent uptake of porcine epidemic diarrhea virus into Vero cells Park, Jung-Eun Cruz, Deu John M. Shin, Hyun-Jin Virus Res Article Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus, is a causative agent of porcine enteric disease characterized by acute watery diarrhea and dehydration in sucking piglet. Similar to other coronaviruses, PEDV spike protein mediates its cell entry by binding to cellular receptors and inducing membrane fusion between viral envelopes and cellular membranes. However, the entry mechanism of PEDV is not studied. Here, we determined the entry mechanism of PEDV into Vero cells. Our data confirmed that PEDV entry followed clathrin-mediated endocytosis independence of caveolae-coated pit assembly. The internalized PEDV was co-localized with the clathrin-mediated endocytic marker, but not with the caveolae-mediated endocytic marker. In addition, cells treated with lysosomotropic agents and serine protease inhibitors were resistant to PEDV. Our data revealed that PEDV entry followed clathrin-mediated endocytosis and was dependent on a low pH and serine proteolysis for successful entry into cells. Elsevier B.V. 2014-10-13 2014-07-31 /pmc/articles/PMC7114442/ /pubmed/25086180 http://dx.doi.org/10.1016/j.virusres.2014.07.022 Text en Copyright © 2014 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Park, Jung-Eun
Cruz, Deu John M.
Shin, Hyun-Jin
Clathrin- and serine proteases-dependent uptake of porcine epidemic diarrhea virus into Vero cells
title Clathrin- and serine proteases-dependent uptake of porcine epidemic diarrhea virus into Vero cells
title_full Clathrin- and serine proteases-dependent uptake of porcine epidemic diarrhea virus into Vero cells
title_fullStr Clathrin- and serine proteases-dependent uptake of porcine epidemic diarrhea virus into Vero cells
title_full_unstemmed Clathrin- and serine proteases-dependent uptake of porcine epidemic diarrhea virus into Vero cells
title_short Clathrin- and serine proteases-dependent uptake of porcine epidemic diarrhea virus into Vero cells
title_sort clathrin- and serine proteases-dependent uptake of porcine epidemic diarrhea virus into vero cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114442/
https://www.ncbi.nlm.nih.gov/pubmed/25086180
http://dx.doi.org/10.1016/j.virusres.2014.07.022
work_keys_str_mv AT parkjungeun clathrinandserineproteasesdependentuptakeofporcineepidemicdiarrheavirusintoverocells
AT cruzdeujohnm clathrinandserineproteasesdependentuptakeofporcineepidemicdiarrheavirusintoverocells
AT shinhyunjin clathrinandserineproteasesdependentuptakeofporcineepidemicdiarrheavirusintoverocells