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Interleukin-1β expression by a recombinant porcine reproductive and respiratory syndrome virus
The cytokine interleukin-1 beta (IL-1β) is a potent inflammatory mediator in response to infection, and can be used as an immunological adjuvant. In this study, we constructed a recombinant porcine reproductive and respiratory syndrome virus (vP129/swIL1β) expressing swine IL-1β from the separate su...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114469/ https://www.ncbi.nlm.nih.gov/pubmed/22119401 http://dx.doi.org/10.1016/j.virusres.2011.11.007 |
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author | Lawson, Steven R. Li, Yanhua Patton, John B. Langenhorst, Robert J. Sun, Zhi Jiang, Zhiyong Christopher-Hennings, Jane Nelson, Eric A. Knudsen, David Fang, Ying Chang, Kyeong-Ok |
author_facet | Lawson, Steven R. Li, Yanhua Patton, John B. Langenhorst, Robert J. Sun, Zhi Jiang, Zhiyong Christopher-Hennings, Jane Nelson, Eric A. Knudsen, David Fang, Ying Chang, Kyeong-Ok |
author_sort | Lawson, Steven R. |
collection | PubMed |
description | The cytokine interleukin-1 beta (IL-1β) is a potent inflammatory mediator in response to infection, and can be used as an immunological adjuvant. In this study, we constructed a recombinant porcine reproductive and respiratory syndrome virus (vP129/swIL1β) expressing swine IL-1β from the separate subgenomic mRNA inserted between the ORF1b and ORF2 genome region. MARC-145 cells infected with vP129/swIL1β secreted 1947 pg of IL-1β per 2 × 10(5) cells at 36 h post-infection. In vitro growth kinetics analysis in MARC-145 cells showed that the vP129/swIL1β virus had a similar replication rate as that of parental virus. We further performed in vivo characterization of the vP129/swIL1β virus in a nursery pig disease model. The vP129/swIL1β infected pigs did not show visible clinical signs, while respiratory distress and lethargy were evident in pigs infected with the parental virus. The expression of various cytokines from peripheral blood mononuclear cells measured by fluorescent microsphere immunoassay showed that IL-1β, IL-4 and IFN-γ expression levels were up-regulated in pigs infected with vP129/swIL1β at 7 and 14 days post-infection. However, no detectable level of IL-1β was found in serum samples from pigs infected with either vP129/swIL1β or parental virus. In summary, this study demonstrated a recombinant PRRSV as a useful tool to study the role of different cytokines in disease progression and immune responses, which represents a new strategy for future therapeutic application and vaccine development. |
format | Online Article Text |
id | pubmed-7114469 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71144692020-04-02 Interleukin-1β expression by a recombinant porcine reproductive and respiratory syndrome virus Lawson, Steven R. Li, Yanhua Patton, John B. Langenhorst, Robert J. Sun, Zhi Jiang, Zhiyong Christopher-Hennings, Jane Nelson, Eric A. Knudsen, David Fang, Ying Chang, Kyeong-Ok Virus Res Article The cytokine interleukin-1 beta (IL-1β) is a potent inflammatory mediator in response to infection, and can be used as an immunological adjuvant. In this study, we constructed a recombinant porcine reproductive and respiratory syndrome virus (vP129/swIL1β) expressing swine IL-1β from the separate subgenomic mRNA inserted between the ORF1b and ORF2 genome region. MARC-145 cells infected with vP129/swIL1β secreted 1947 pg of IL-1β per 2 × 10(5) cells at 36 h post-infection. In vitro growth kinetics analysis in MARC-145 cells showed that the vP129/swIL1β virus had a similar replication rate as that of parental virus. We further performed in vivo characterization of the vP129/swIL1β virus in a nursery pig disease model. The vP129/swIL1β infected pigs did not show visible clinical signs, while respiratory distress and lethargy were evident in pigs infected with the parental virus. The expression of various cytokines from peripheral blood mononuclear cells measured by fluorescent microsphere immunoassay showed that IL-1β, IL-4 and IFN-γ expression levels were up-regulated in pigs infected with vP129/swIL1β at 7 and 14 days post-infection. However, no detectable level of IL-1β was found in serum samples from pigs infected with either vP129/swIL1β or parental virus. In summary, this study demonstrated a recombinant PRRSV as a useful tool to study the role of different cytokines in disease progression and immune responses, which represents a new strategy for future therapeutic application and vaccine development. Elsevier B.V. 2012-02 2011-11-19 /pmc/articles/PMC7114469/ /pubmed/22119401 http://dx.doi.org/10.1016/j.virusres.2011.11.007 Text en Copyright © 2011 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Lawson, Steven R. Li, Yanhua Patton, John B. Langenhorst, Robert J. Sun, Zhi Jiang, Zhiyong Christopher-Hennings, Jane Nelson, Eric A. Knudsen, David Fang, Ying Chang, Kyeong-Ok Interleukin-1β expression by a recombinant porcine reproductive and respiratory syndrome virus |
title | Interleukin-1β expression by a recombinant porcine reproductive and respiratory syndrome virus |
title_full | Interleukin-1β expression by a recombinant porcine reproductive and respiratory syndrome virus |
title_fullStr | Interleukin-1β expression by a recombinant porcine reproductive and respiratory syndrome virus |
title_full_unstemmed | Interleukin-1β expression by a recombinant porcine reproductive and respiratory syndrome virus |
title_short | Interleukin-1β expression by a recombinant porcine reproductive and respiratory syndrome virus |
title_sort | interleukin-1β expression by a recombinant porcine reproductive and respiratory syndrome virus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114469/ https://www.ncbi.nlm.nih.gov/pubmed/22119401 http://dx.doi.org/10.1016/j.virusres.2011.11.007 |
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