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Reprint of: Coronavirus reverse genetic systems: Infectious clones and replicons()
Coronaviruses (CoVs) infect humans and many animal species, and are associated with respiratory, enteric, hepatic, and central nervous system diseases. The large size of the CoV genome and the instability of some CoV replicase gene sequences during its propagation in bacteria, represent serious obst...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114485/ https://www.ncbi.nlm.nih.gov/pubmed/25261606 http://dx.doi.org/10.1016/j.virusres.2014.09.006 |
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author | Almazán, Fernando Sola, Isabel Zuñiga, Sonia Marquez-Jurado, Silvia Morales, Lucia Becares, Martina Enjuanes, Luis |
author_facet | Almazán, Fernando Sola, Isabel Zuñiga, Sonia Marquez-Jurado, Silvia Morales, Lucia Becares, Martina Enjuanes, Luis |
author_sort | Almazán, Fernando |
collection | PubMed |
description | Coronaviruses (CoVs) infect humans and many animal species, and are associated with respiratory, enteric, hepatic, and central nervous system diseases. The large size of the CoV genome and the instability of some CoV replicase gene sequences during its propagation in bacteria, represent serious obstacles for the development of reverse genetic systems similar to those used for smaller positive sense RNA viruses. To overcome these limitations, several alternatives to more conventional plasmid-based approaches have been established in the last 13 years. In this report, we briefly review and discuss the different reverse genetic systems developed for CoVs, paying special attention to the severe acute respiratory syndrome CoV (SARS-CoV). |
format | Online Article Text |
id | pubmed-7114485 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71144852020-04-02 Reprint of: Coronavirus reverse genetic systems: Infectious clones and replicons() Almazán, Fernando Sola, Isabel Zuñiga, Sonia Marquez-Jurado, Silvia Morales, Lucia Becares, Martina Enjuanes, Luis Virus Res Article Coronaviruses (CoVs) infect humans and many animal species, and are associated with respiratory, enteric, hepatic, and central nervous system diseases. The large size of the CoV genome and the instability of some CoV replicase gene sequences during its propagation in bacteria, represent serious obstacles for the development of reverse genetic systems similar to those used for smaller positive sense RNA viruses. To overcome these limitations, several alternatives to more conventional plasmid-based approaches have been established in the last 13 years. In this report, we briefly review and discuss the different reverse genetic systems developed for CoVs, paying special attention to the severe acute respiratory syndrome CoV (SARS-CoV). Elsevier B.V. 2014-12-19 2014-09-26 /pmc/articles/PMC7114485/ /pubmed/25261606 http://dx.doi.org/10.1016/j.virusres.2014.09.006 Text en Copyright © 2014 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Almazán, Fernando Sola, Isabel Zuñiga, Sonia Marquez-Jurado, Silvia Morales, Lucia Becares, Martina Enjuanes, Luis Reprint of: Coronavirus reverse genetic systems: Infectious clones and replicons() |
title | Reprint of: Coronavirus reverse genetic systems: Infectious clones and replicons() |
title_full | Reprint of: Coronavirus reverse genetic systems: Infectious clones and replicons() |
title_fullStr | Reprint of: Coronavirus reverse genetic systems: Infectious clones and replicons() |
title_full_unstemmed | Reprint of: Coronavirus reverse genetic systems: Infectious clones and replicons() |
title_short | Reprint of: Coronavirus reverse genetic systems: Infectious clones and replicons() |
title_sort | reprint of: coronavirus reverse genetic systems: infectious clones and replicons() |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114485/ https://www.ncbi.nlm.nih.gov/pubmed/25261606 http://dx.doi.org/10.1016/j.virusres.2014.09.006 |
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