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Porcine epidemic diarrhea virus infects and replicates in porcine alveolar macrophages

Porcine epidemic diarrhea virus (PEDV) is a causative agent of porcine epidemic diarrhea; consequently, the small intestine was believed to be its only target organ. In this study, we found that PEDV infected not only the small intestines, but also the respiratory tract. Infection and replication of...

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Autores principales: Park, Jung-Eun, Shin, Hyun-Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114489/
https://www.ncbi.nlm.nih.gov/pubmed/25116392
http://dx.doi.org/10.1016/j.virusres.2014.07.038
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author Park, Jung-Eun
Shin, Hyun-Jin
author_facet Park, Jung-Eun
Shin, Hyun-Jin
author_sort Park, Jung-Eun
collection PubMed
description Porcine epidemic diarrhea virus (PEDV) is a causative agent of porcine epidemic diarrhea; consequently, the small intestine was believed to be its only target organ. In this study, we found that PEDV infected not only the small intestines, but also the respiratory tract. Infection and replication of PEDV in the respiratory tract from naturally PEDV-infected piglets were examined by reverse transcription polymerase chain reaction, immunohistochemistry, and virus re-isolation. Our observations were confirmed by experimental inoculation, and we found that PEDV infection in the respiratory tract was specifically associated with alveolar macrophages in the lung. Vero cell-adapted PEDV was able to replicate in both primary alveolar macrophages and continuous porcine alveolar macrophage cells. Sequencing analysis of the spike (S) glycoprotein revealed that mutations in S might be a potential determinant of auxiliary targets for PEDV. The discovery that PEDV infects and replicates in alveolar macrophages provides new insights into its pathogenesis.
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spelling pubmed-71144892020-04-02 Porcine epidemic diarrhea virus infects and replicates in porcine alveolar macrophages Park, Jung-Eun Shin, Hyun-Jin Virus Res Article Porcine epidemic diarrhea virus (PEDV) is a causative agent of porcine epidemic diarrhea; consequently, the small intestine was believed to be its only target organ. In this study, we found that PEDV infected not only the small intestines, but also the respiratory tract. Infection and replication of PEDV in the respiratory tract from naturally PEDV-infected piglets were examined by reverse transcription polymerase chain reaction, immunohistochemistry, and virus re-isolation. Our observations were confirmed by experimental inoculation, and we found that PEDV infection in the respiratory tract was specifically associated with alveolar macrophages in the lung. Vero cell-adapted PEDV was able to replicate in both primary alveolar macrophages and continuous porcine alveolar macrophage cells. Sequencing analysis of the spike (S) glycoprotein revealed that mutations in S might be a potential determinant of auxiliary targets for PEDV. The discovery that PEDV infects and replicates in alveolar macrophages provides new insights into its pathogenesis. Elsevier B.V. 2014-10-13 2014-08-10 /pmc/articles/PMC7114489/ /pubmed/25116392 http://dx.doi.org/10.1016/j.virusres.2014.07.038 Text en Copyright © 2014 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Park, Jung-Eun
Shin, Hyun-Jin
Porcine epidemic diarrhea virus infects and replicates in porcine alveolar macrophages
title Porcine epidemic diarrhea virus infects and replicates in porcine alveolar macrophages
title_full Porcine epidemic diarrhea virus infects and replicates in porcine alveolar macrophages
title_fullStr Porcine epidemic diarrhea virus infects and replicates in porcine alveolar macrophages
title_full_unstemmed Porcine epidemic diarrhea virus infects and replicates in porcine alveolar macrophages
title_short Porcine epidemic diarrhea virus infects and replicates in porcine alveolar macrophages
title_sort porcine epidemic diarrhea virus infects and replicates in porcine alveolar macrophages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114489/
https://www.ncbi.nlm.nih.gov/pubmed/25116392
http://dx.doi.org/10.1016/j.virusres.2014.07.038
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