Replication of human coronaviruses SARS-CoV, HCoV-NL63 and HCoV-229E is inhibited by the drug FK506

Recent research has shown that Coronavirus (CoV) replication depends on active immunophilin pathways. Here we demonstrate that the drug FK506 (Tacrolimus) inhibited strongly the growth of human coronaviruses SARS-CoV, HCoV-NL63 and HCoV-229E at low, non-cytotoxic concentrations in cell culture. As s...

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Autores principales: Carbajo-Lozoya, Javier, Müller, Marcel A., Kallies, Stephan, Thiel, Volker, Drosten, Christian, von Brunn, Albrecht
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114512/
https://www.ncbi.nlm.nih.gov/pubmed/22349148
http://dx.doi.org/10.1016/j.virusres.2012.02.002
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author Carbajo-Lozoya, Javier
Müller, Marcel A.
Kallies, Stephan
Thiel, Volker
Drosten, Christian
von Brunn, Albrecht
author_facet Carbajo-Lozoya, Javier
Müller, Marcel A.
Kallies, Stephan
Thiel, Volker
Drosten, Christian
von Brunn, Albrecht
author_sort Carbajo-Lozoya, Javier
collection PubMed
description Recent research has shown that Coronavirus (CoV) replication depends on active immunophilin pathways. Here we demonstrate that the drug FK506 (Tacrolimus) inhibited strongly the growth of human coronaviruses SARS-CoV, HCoV-NL63 and HCoV-229E at low, non-cytotoxic concentrations in cell culture. As shown by plaque titration, qPCR, Luciferase- and green fluorescent protein (GFP) reporter gene expression, replication was diminished by several orders of magnitude. Knockdown of the cellular FK506-binding proteins FKBP1A and FKBP1B in CaCo2 cells prevented replication of HCoV-NL63, suggesting the requirement of these members of the immunophilin family for virus growth.
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spelling pubmed-71145122020-04-02 Replication of human coronaviruses SARS-CoV, HCoV-NL63 and HCoV-229E is inhibited by the drug FK506 Carbajo-Lozoya, Javier Müller, Marcel A. Kallies, Stephan Thiel, Volker Drosten, Christian von Brunn, Albrecht Virus Res Article Recent research has shown that Coronavirus (CoV) replication depends on active immunophilin pathways. Here we demonstrate that the drug FK506 (Tacrolimus) inhibited strongly the growth of human coronaviruses SARS-CoV, HCoV-NL63 and HCoV-229E at low, non-cytotoxic concentrations in cell culture. As shown by plaque titration, qPCR, Luciferase- and green fluorescent protein (GFP) reporter gene expression, replication was diminished by several orders of magnitude. Knockdown of the cellular FK506-binding proteins FKBP1A and FKBP1B in CaCo2 cells prevented replication of HCoV-NL63, suggesting the requirement of these members of the immunophilin family for virus growth. Elsevier B.V. 2012-04 2012-02-10 /pmc/articles/PMC7114512/ /pubmed/22349148 http://dx.doi.org/10.1016/j.virusres.2012.02.002 Text en Copyright © 2012 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Carbajo-Lozoya, Javier
Müller, Marcel A.
Kallies, Stephan
Thiel, Volker
Drosten, Christian
von Brunn, Albrecht
Replication of human coronaviruses SARS-CoV, HCoV-NL63 and HCoV-229E is inhibited by the drug FK506
title Replication of human coronaviruses SARS-CoV, HCoV-NL63 and HCoV-229E is inhibited by the drug FK506
title_full Replication of human coronaviruses SARS-CoV, HCoV-NL63 and HCoV-229E is inhibited by the drug FK506
title_fullStr Replication of human coronaviruses SARS-CoV, HCoV-NL63 and HCoV-229E is inhibited by the drug FK506
title_full_unstemmed Replication of human coronaviruses SARS-CoV, HCoV-NL63 and HCoV-229E is inhibited by the drug FK506
title_short Replication of human coronaviruses SARS-CoV, HCoV-NL63 and HCoV-229E is inhibited by the drug FK506
title_sort replication of human coronaviruses sars-cov, hcov-nl63 and hcov-229e is inhibited by the drug fk506
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114512/
https://www.ncbi.nlm.nih.gov/pubmed/22349148
http://dx.doi.org/10.1016/j.virusres.2012.02.002
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