The p7 protein of the hepatitis C virus induces cell death differently from the influenza A virus viroporin M2
Most viruses encode proteins that modulate cell-death signaling by the host. For hepatitis C virus (HCV) infection, apoptosis and other forms of cell-death have been observed in vitro and in vivo but the detailed understanding of this intricate viral-host interplay is unclear. This study examined th...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114515/ https://www.ncbi.nlm.nih.gov/pubmed/23246447 http://dx.doi.org/10.1016/j.virusres.2012.12.005 |
_version_ | 1783513903232188416 |
---|---|
author | Aweya, Jude Juventus Mak, Tze Minn Lim, Seng Gee Tan, Yee-Joo |
author_facet | Aweya, Jude Juventus Mak, Tze Minn Lim, Seng Gee Tan, Yee-Joo |
author_sort | Aweya, Jude Juventus |
collection | PubMed |
description | Most viruses encode proteins that modulate cell-death signaling by the host. For hepatitis C virus (HCV) infection, apoptosis and other forms of cell-death have been observed in vitro and in vivo but the detailed understanding of this intricate viral-host interplay is unclear. This study examined the role played by the HCV p7 protein in the induction of cell-death. By measuring caspase-3/7 activation and cleavage of endogenous PARP, two hallmarks of apoptosis, the overexpression of p7 protein was shown to induce apoptosis in Huh7.5 cells. Furthermore, p7-induced apoptosis is caspase-dependent and involves both the intrinsic and extrinsic pathways. Similar to the M2 protein of influenza A virus, p7-induced apoptosis is independent of its ion channel activity. Coimmunoprecipitation experiments further showed that both M2 and p7 interact with the essential autophagy protein Beclin-1. However, only the M2 protein could cause an increase in the level of LC3-II, which is an indicator of autophagic activity. Thus, although the p7 protein is functionally similar to the well-characterized M2 protein, they differ in their activation of autophagic cell-death. Taken together, these results shed more light on the relationship between the HCV p7 ion channel protein and cell-death induction in host cells. |
format | Online Article Text |
id | pubmed-7114515 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71145152020-04-02 The p7 protein of the hepatitis C virus induces cell death differently from the influenza A virus viroporin M2 Aweya, Jude Juventus Mak, Tze Minn Lim, Seng Gee Tan, Yee-Joo Virus Res Article Most viruses encode proteins that modulate cell-death signaling by the host. For hepatitis C virus (HCV) infection, apoptosis and other forms of cell-death have been observed in vitro and in vivo but the detailed understanding of this intricate viral-host interplay is unclear. This study examined the role played by the HCV p7 protein in the induction of cell-death. By measuring caspase-3/7 activation and cleavage of endogenous PARP, two hallmarks of apoptosis, the overexpression of p7 protein was shown to induce apoptosis in Huh7.5 cells. Furthermore, p7-induced apoptosis is caspase-dependent and involves both the intrinsic and extrinsic pathways. Similar to the M2 protein of influenza A virus, p7-induced apoptosis is independent of its ion channel activity. Coimmunoprecipitation experiments further showed that both M2 and p7 interact with the essential autophagy protein Beclin-1. However, only the M2 protein could cause an increase in the level of LC3-II, which is an indicator of autophagic activity. Thus, although the p7 protein is functionally similar to the well-characterized M2 protein, they differ in their activation of autophagic cell-death. Taken together, these results shed more light on the relationship between the HCV p7 ion channel protein and cell-death induction in host cells. Elsevier B.V. 2013-03 2012-12-12 /pmc/articles/PMC7114515/ /pubmed/23246447 http://dx.doi.org/10.1016/j.virusres.2012.12.005 Text en Copyright © 2012 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Aweya, Jude Juventus Mak, Tze Minn Lim, Seng Gee Tan, Yee-Joo The p7 protein of the hepatitis C virus induces cell death differently from the influenza A virus viroporin M2 |
title | The p7 protein of the hepatitis C virus induces cell death differently from the influenza A virus viroporin M2 |
title_full | The p7 protein of the hepatitis C virus induces cell death differently from the influenza A virus viroporin M2 |
title_fullStr | The p7 protein of the hepatitis C virus induces cell death differently from the influenza A virus viroporin M2 |
title_full_unstemmed | The p7 protein of the hepatitis C virus induces cell death differently from the influenza A virus viroporin M2 |
title_short | The p7 protein of the hepatitis C virus induces cell death differently from the influenza A virus viroporin M2 |
title_sort | p7 protein of the hepatitis c virus induces cell death differently from the influenza a virus viroporin m2 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114515/ https://www.ncbi.nlm.nih.gov/pubmed/23246447 http://dx.doi.org/10.1016/j.virusres.2012.12.005 |
work_keys_str_mv | AT aweyajudejuventus thep7proteinofthehepatitiscvirusinducescelldeathdifferentlyfromtheinfluenzaavirusviroporinm2 AT maktzeminn thep7proteinofthehepatitiscvirusinducescelldeathdifferentlyfromtheinfluenzaavirusviroporinm2 AT limsenggee thep7proteinofthehepatitiscvirusinducescelldeathdifferentlyfromtheinfluenzaavirusviroporinm2 AT tanyeejoo thep7proteinofthehepatitiscvirusinducescelldeathdifferentlyfromtheinfluenzaavirusviroporinm2 AT aweyajudejuventus p7proteinofthehepatitiscvirusinducescelldeathdifferentlyfromtheinfluenzaavirusviroporinm2 AT maktzeminn p7proteinofthehepatitiscvirusinducescelldeathdifferentlyfromtheinfluenzaavirusviroporinm2 AT limsenggee p7proteinofthehepatitiscvirusinducescelldeathdifferentlyfromtheinfluenzaavirusviroporinm2 AT tanyeejoo p7proteinofthehepatitiscvirusinducescelldeathdifferentlyfromtheinfluenzaavirusviroporinm2 |