Cargando…
Foot and mouth disease virus undergoes non-progressive replication in mice peritoneal macrophages and induces M1 polarization
Despite the fact that macrophages link the innate and adaptive arms of immunity, it’s role in the early infection of foot and mouth disease virus (FMDV) is largely unknown. Recently, depletion of macrophages in vivo after vaccination has shown to drastically diminish the protection against FMDV chal...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier B.V.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114663/ https://www.ncbi.nlm.nih.gov/pubmed/32109526 http://dx.doi.org/10.1016/j.virusres.2020.197906 |
_version_ | 1783513936282255360 |
---|---|
author | Sebastian, Renjith Sravanthi, M. Umapathi, V. Krishnaswamy, N Priyanka, M. Dechamma, H.J. Ganesh, K. Basagoudanavar, Suresh H. Sanyal, A. Reddy, G.R. |
author_facet | Sebastian, Renjith Sravanthi, M. Umapathi, V. Krishnaswamy, N Priyanka, M. Dechamma, H.J. Ganesh, K. Basagoudanavar, Suresh H. Sanyal, A. Reddy, G.R. |
author_sort | Sebastian, Renjith |
collection | PubMed |
description | Despite the fact that macrophages link the innate and adaptive arms of immunity, it’s role in the early infection of foot and mouth disease virus (FMDV) is largely unknown. Recently, depletion of macrophages in vivo after vaccination has shown to drastically diminish the protection against FMDV challenge in mouse model. Even the ability of macrophages to reduce or resist FMDV infection is not known hitherto. Therefore, we examined the replication ability of FMDV in mice peritoneal macrophages and the responsiveness in terms of macrophage polarization and cytokine production. Negative strand specific RT-PCR indicated replication of FMDV RNA in macrophages. Absolute quantitation of FMDV transcripts, immunofluorescence studies and titre of the infectious progeny virus revealed that replication peaked at 12 hpi and significantly declined by 18 hpi indicating non-progressive replication in the infected macrophages. Further, significant up regulation of inducible nitric oxide synthase by 8 –12 hpi and increase of M1 specific CD11c + cells by 42.6 % after infection showed that FMDV induce M1 polarization. A significant up regulation of TNFα and IL12 transcripts at 8 hpi supported that M1 macrophages were functional. Further, we studied the expression of Type I to III interferons (IFN) and other antiviral molecules. The results indicate a marked up regulation of Type I IFNα and β by 9.2 and 11.2 fold, respectively at 8 hpi. Of the four IFN stimulated genes (ISG), viperin showed a significant up regulation by 286-fold at 12 hpi in the mice macrophages. In conclusion, the results suggest that replication of FMDV in mice peritoneal macrophages is non-progressive with up regulation of Type I IFN and ISGs. Further, FMDV induces M1 polarization in murine peritoneal macrophages. |
format | Online Article Text |
id | pubmed-7114663 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Published by Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71146632020-04-02 Foot and mouth disease virus undergoes non-progressive replication in mice peritoneal macrophages and induces M1 polarization Sebastian, Renjith Sravanthi, M. Umapathi, V. Krishnaswamy, N Priyanka, M. Dechamma, H.J. Ganesh, K. Basagoudanavar, Suresh H. Sanyal, A. Reddy, G.R. Virus Res Article Despite the fact that macrophages link the innate and adaptive arms of immunity, it’s role in the early infection of foot and mouth disease virus (FMDV) is largely unknown. Recently, depletion of macrophages in vivo after vaccination has shown to drastically diminish the protection against FMDV challenge in mouse model. Even the ability of macrophages to reduce or resist FMDV infection is not known hitherto. Therefore, we examined the replication ability of FMDV in mice peritoneal macrophages and the responsiveness in terms of macrophage polarization and cytokine production. Negative strand specific RT-PCR indicated replication of FMDV RNA in macrophages. Absolute quantitation of FMDV transcripts, immunofluorescence studies and titre of the infectious progeny virus revealed that replication peaked at 12 hpi and significantly declined by 18 hpi indicating non-progressive replication in the infected macrophages. Further, significant up regulation of inducible nitric oxide synthase by 8 –12 hpi and increase of M1 specific CD11c + cells by 42.6 % after infection showed that FMDV induce M1 polarization. A significant up regulation of TNFα and IL12 transcripts at 8 hpi supported that M1 macrophages were functional. Further, we studied the expression of Type I to III interferons (IFN) and other antiviral molecules. The results indicate a marked up regulation of Type I IFNα and β by 9.2 and 11.2 fold, respectively at 8 hpi. Of the four IFN stimulated genes (ISG), viperin showed a significant up regulation by 286-fold at 12 hpi in the mice macrophages. In conclusion, the results suggest that replication of FMDV in mice peritoneal macrophages is non-progressive with up regulation of Type I IFN and ISGs. Further, FMDV induces M1 polarization in murine peritoneal macrophages. Published by Elsevier B.V. 2020-05 2020-02-25 /pmc/articles/PMC7114663/ /pubmed/32109526 http://dx.doi.org/10.1016/j.virusres.2020.197906 Text en © 2020 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Sebastian, Renjith Sravanthi, M. Umapathi, V. Krishnaswamy, N Priyanka, M. Dechamma, H.J. Ganesh, K. Basagoudanavar, Suresh H. Sanyal, A. Reddy, G.R. Foot and mouth disease virus undergoes non-progressive replication in mice peritoneal macrophages and induces M1 polarization |
title | Foot and mouth disease virus undergoes non-progressive replication in mice peritoneal macrophages and induces M1 polarization |
title_full | Foot and mouth disease virus undergoes non-progressive replication in mice peritoneal macrophages and induces M1 polarization |
title_fullStr | Foot and mouth disease virus undergoes non-progressive replication in mice peritoneal macrophages and induces M1 polarization |
title_full_unstemmed | Foot and mouth disease virus undergoes non-progressive replication in mice peritoneal macrophages and induces M1 polarization |
title_short | Foot and mouth disease virus undergoes non-progressive replication in mice peritoneal macrophages and induces M1 polarization |
title_sort | foot and mouth disease virus undergoes non-progressive replication in mice peritoneal macrophages and induces m1 polarization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114663/ https://www.ncbi.nlm.nih.gov/pubmed/32109526 http://dx.doi.org/10.1016/j.virusres.2020.197906 |
work_keys_str_mv | AT sebastianrenjith footandmouthdiseasevirusundergoesnonprogressivereplicationinmiceperitonealmacrophagesandinducesm1polarization AT sravanthim footandmouthdiseasevirusundergoesnonprogressivereplicationinmiceperitonealmacrophagesandinducesm1polarization AT umapathiv footandmouthdiseasevirusundergoesnonprogressivereplicationinmiceperitonealmacrophagesandinducesm1polarization AT krishnaswamyn footandmouthdiseasevirusundergoesnonprogressivereplicationinmiceperitonealmacrophagesandinducesm1polarization AT priyankam footandmouthdiseasevirusundergoesnonprogressivereplicationinmiceperitonealmacrophagesandinducesm1polarization AT dechammahj footandmouthdiseasevirusundergoesnonprogressivereplicationinmiceperitonealmacrophagesandinducesm1polarization AT ganeshk footandmouthdiseasevirusundergoesnonprogressivereplicationinmiceperitonealmacrophagesandinducesm1polarization AT basagoudanavarsureshh footandmouthdiseasevirusundergoesnonprogressivereplicationinmiceperitonealmacrophagesandinducesm1polarization AT sanyala footandmouthdiseasevirusundergoesnonprogressivereplicationinmiceperitonealmacrophagesandinducesm1polarization AT reddygr footandmouthdiseasevirusundergoesnonprogressivereplicationinmiceperitonealmacrophagesandinducesm1polarization |