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Encephalomyocarditis virus is potentially derived from eastern bent-wing bats living in East Asian countries
Bats are reservoir hosts of many zoonotic viruses and identification of viruses that they carry is important. This study aimed to use high throughput screening to identify the viruses in fecal guano of Taiwanese insectivorous bats caves in order to obtain more information on bat-derived pathogenic v...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114854/ https://www.ncbi.nlm.nih.gov/pubmed/30391400 http://dx.doi.org/10.1016/j.virusres.2018.10.020 |
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author | Doysabas, Karla Cristine C. Oba, Mami Furuta, Masaya Iida, Keisuke Omatsu, Tsutomu Furuya, Tetsuya Okada, Takashi Sutummaporn, Kripitch Shimoda, Hiroshi Wong, Min-Liang Wu, Chung-Hsin Ohmori, Yasushige Kobayashi, Ryosuke Hengjan, Yupadee Yonemitsu, Kenzo Kuwata, Ryusei Kim, Yoo-Kyung Han, Sang-Hyun Sohn, Joon-Hyuk Han, Sang-Hoon Suzuki, Kazuo Kimura, Junpei Maeda, Ken Oh, Hong-Shik Endoh, Daiji Mizutani, Tetsuya Hondo, Eiichi |
author_facet | Doysabas, Karla Cristine C. Oba, Mami Furuta, Masaya Iida, Keisuke Omatsu, Tsutomu Furuya, Tetsuya Okada, Takashi Sutummaporn, Kripitch Shimoda, Hiroshi Wong, Min-Liang Wu, Chung-Hsin Ohmori, Yasushige Kobayashi, Ryosuke Hengjan, Yupadee Yonemitsu, Kenzo Kuwata, Ryusei Kim, Yoo-Kyung Han, Sang-Hyun Sohn, Joon-Hyuk Han, Sang-Hoon Suzuki, Kazuo Kimura, Junpei Maeda, Ken Oh, Hong-Shik Endoh, Daiji Mizutani, Tetsuya Hondo, Eiichi |
author_sort | Doysabas, Karla Cristine C. |
collection | PubMed |
description | Bats are reservoir hosts of many zoonotic viruses and identification of viruses that they carry is important. This study aimed to use high throughput screening to identify the viruses in fecal guano of Taiwanese insectivorous bats caves in order to obtain more information on bat-derived pathogenic viruses in East Asia. Guano samples were collected from two caves in Taiwan, pooled, and then subjected to Multiplex PCR-based next generation sequencing for viral identification. Subsequently, encephalomyocarditis virus (EMCV) sequence was detected and confirmed by reverse transcription PCR. EMCV is considered as rodent virus and thus, animal species identification through cytochrome oxidase I (COI) barcoding was further done to identify the viral source. Finally, determination of distribution and verification of the presence of EMCV in guano obtained from Japanese and South Korean caves was also done. We concluded that the guano collected was not contaminated with the excrement of rodents which were reported and presumed to live in Taiwan. Also, EMCV genome fragments were found in guanos of Japanese and South Korean caves. It is possible that the eastern bent-wing bat (Miniopterus fuliginosus) is one of the natural hosts of EMCV in East Asia. |
format | Online Article Text |
id | pubmed-7114854 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71148542020-04-02 Encephalomyocarditis virus is potentially derived from eastern bent-wing bats living in East Asian countries Doysabas, Karla Cristine C. Oba, Mami Furuta, Masaya Iida, Keisuke Omatsu, Tsutomu Furuya, Tetsuya Okada, Takashi Sutummaporn, Kripitch Shimoda, Hiroshi Wong, Min-Liang Wu, Chung-Hsin Ohmori, Yasushige Kobayashi, Ryosuke Hengjan, Yupadee Yonemitsu, Kenzo Kuwata, Ryusei Kim, Yoo-Kyung Han, Sang-Hyun Sohn, Joon-Hyuk Han, Sang-Hoon Suzuki, Kazuo Kimura, Junpei Maeda, Ken Oh, Hong-Shik Endoh, Daiji Mizutani, Tetsuya Hondo, Eiichi Virus Res Article Bats are reservoir hosts of many zoonotic viruses and identification of viruses that they carry is important. This study aimed to use high throughput screening to identify the viruses in fecal guano of Taiwanese insectivorous bats caves in order to obtain more information on bat-derived pathogenic viruses in East Asia. Guano samples were collected from two caves in Taiwan, pooled, and then subjected to Multiplex PCR-based next generation sequencing for viral identification. Subsequently, encephalomyocarditis virus (EMCV) sequence was detected and confirmed by reverse transcription PCR. EMCV is considered as rodent virus and thus, animal species identification through cytochrome oxidase I (COI) barcoding was further done to identify the viral source. Finally, determination of distribution and verification of the presence of EMCV in guano obtained from Japanese and South Korean caves was also done. We concluded that the guano collected was not contaminated with the excrement of rodents which were reported and presumed to live in Taiwan. Also, EMCV genome fragments were found in guanos of Japanese and South Korean caves. It is possible that the eastern bent-wing bat (Miniopterus fuliginosus) is one of the natural hosts of EMCV in East Asia. Elsevier B.V. 2019-01-02 2018-11-01 /pmc/articles/PMC7114854/ /pubmed/30391400 http://dx.doi.org/10.1016/j.virusres.2018.10.020 Text en © 2018 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Doysabas, Karla Cristine C. Oba, Mami Furuta, Masaya Iida, Keisuke Omatsu, Tsutomu Furuya, Tetsuya Okada, Takashi Sutummaporn, Kripitch Shimoda, Hiroshi Wong, Min-Liang Wu, Chung-Hsin Ohmori, Yasushige Kobayashi, Ryosuke Hengjan, Yupadee Yonemitsu, Kenzo Kuwata, Ryusei Kim, Yoo-Kyung Han, Sang-Hyun Sohn, Joon-Hyuk Han, Sang-Hoon Suzuki, Kazuo Kimura, Junpei Maeda, Ken Oh, Hong-Shik Endoh, Daiji Mizutani, Tetsuya Hondo, Eiichi Encephalomyocarditis virus is potentially derived from eastern bent-wing bats living in East Asian countries |
title | Encephalomyocarditis virus is potentially derived from eastern bent-wing bats living in East Asian countries |
title_full | Encephalomyocarditis virus is potentially derived from eastern bent-wing bats living in East Asian countries |
title_fullStr | Encephalomyocarditis virus is potentially derived from eastern bent-wing bats living in East Asian countries |
title_full_unstemmed | Encephalomyocarditis virus is potentially derived from eastern bent-wing bats living in East Asian countries |
title_short | Encephalomyocarditis virus is potentially derived from eastern bent-wing bats living in East Asian countries |
title_sort | encephalomyocarditis virus is potentially derived from eastern bent-wing bats living in east asian countries |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114854/ https://www.ncbi.nlm.nih.gov/pubmed/30391400 http://dx.doi.org/10.1016/j.virusres.2018.10.020 |
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