Cargando…

Pilot study and bioinformatics analysis of differentially expressed genes in adipose tissues of rats with excess dietary intake

Excessive adipose tissue accumulation is an increasing health problem worldwide. The present study aimed to determine differentially expressed genes (DEGs) that are associated with the excessive accumulation of adipose tissues by PCR arrays in an excess dietary intake animal model. For this purpose,...

Descripción completa

Detalles Bibliográficos
Autores principales: Yuan, Jun Chao, Yogarajah, Thaneswary, Lim, Shern Kwok, Tee, Get Bee Yvonne, Khoo, Boon Yin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115215/
https://www.ncbi.nlm.nih.gov/pubmed/32323762
http://dx.doi.org/10.3892/mmr.2020.11012
_version_ 1783514052121591808
author Yuan, Jun Chao
Yogarajah, Thaneswary
Lim, Shern Kwok
Tee, Get Bee Yvonne
Khoo, Boon Yin
author_facet Yuan, Jun Chao
Yogarajah, Thaneswary
Lim, Shern Kwok
Tee, Get Bee Yvonne
Khoo, Boon Yin
author_sort Yuan, Jun Chao
collection PubMed
description Excessive adipose tissue accumulation is an increasing health problem worldwide. The present study aimed to determine differentially expressed genes (DEGs) that are associated with the excessive accumulation of adipose tissues by PCR arrays in an excess dietary intake animal model. For this purpose, male Sprague Dawley rats were randomly assigned to 2 groups: Control (given an ordinary diet) and experimental (given twice the amount of the ordinary diet). After 2 months of feeding, the abdominal cavities of the rats from each group were opened, then subcutaneous and visceral adipose tissues were removed. The adipose tissues collected were then used for total RNA extraction and then reverse transcribed to cDNA, which was then used as a template to identify the DEGs of 84 transcripts for rat obesity by RT(2) Profiler PCR Arrays. The results showed significant downregulation of bombesin-like receptor 3 (BRS3) and uncoupling protein 1 (UCP1) in visceral adipose tissues of experimental rats compared with those of the control rats, and differential gene expression analysis showed an association with fat cell differentiation and regulation of triglyceride sequestration, as well as fatty acid binding. The gene expression patterns observed in the present study, which may be associated with peroxisome proliferator-activated receptor-γ (PPARG) on excessive visceral adipose tissue accumulation, may be useful in identifying a group of surrogate biomarkers for the early diet-induced accumulation of visceral adipose tissue detection in humans. The biomarkers can also be the specific targets for drug development to reduce excessive visceral adipose tissue accumulation in the body and its associated diseases.
format Online
Article
Text
id pubmed-7115215
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-71152152020-04-08 Pilot study and bioinformatics analysis of differentially expressed genes in adipose tissues of rats with excess dietary intake Yuan, Jun Chao Yogarajah, Thaneswary Lim, Shern Kwok Tee, Get Bee Yvonne Khoo, Boon Yin Mol Med Rep Articles Excessive adipose tissue accumulation is an increasing health problem worldwide. The present study aimed to determine differentially expressed genes (DEGs) that are associated with the excessive accumulation of adipose tissues by PCR arrays in an excess dietary intake animal model. For this purpose, male Sprague Dawley rats were randomly assigned to 2 groups: Control (given an ordinary diet) and experimental (given twice the amount of the ordinary diet). After 2 months of feeding, the abdominal cavities of the rats from each group were opened, then subcutaneous and visceral adipose tissues were removed. The adipose tissues collected were then used for total RNA extraction and then reverse transcribed to cDNA, which was then used as a template to identify the DEGs of 84 transcripts for rat obesity by RT(2) Profiler PCR Arrays. The results showed significant downregulation of bombesin-like receptor 3 (BRS3) and uncoupling protein 1 (UCP1) in visceral adipose tissues of experimental rats compared with those of the control rats, and differential gene expression analysis showed an association with fat cell differentiation and regulation of triglyceride sequestration, as well as fatty acid binding. The gene expression patterns observed in the present study, which may be associated with peroxisome proliferator-activated receptor-γ (PPARG) on excessive visceral adipose tissue accumulation, may be useful in identifying a group of surrogate biomarkers for the early diet-induced accumulation of visceral adipose tissue detection in humans. The biomarkers can also be the specific targets for drug development to reduce excessive visceral adipose tissue accumulation in the body and its associated diseases. D.A. Spandidos 2020-05 2020-03-04 /pmc/articles/PMC7115215/ /pubmed/32323762 http://dx.doi.org/10.3892/mmr.2020.11012 Text en Copyright: © Yuan et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Yuan, Jun Chao
Yogarajah, Thaneswary
Lim, Shern Kwok
Tee, Get Bee Yvonne
Khoo, Boon Yin
Pilot study and bioinformatics analysis of differentially expressed genes in adipose tissues of rats with excess dietary intake
title Pilot study and bioinformatics analysis of differentially expressed genes in adipose tissues of rats with excess dietary intake
title_full Pilot study and bioinformatics analysis of differentially expressed genes in adipose tissues of rats with excess dietary intake
title_fullStr Pilot study and bioinformatics analysis of differentially expressed genes in adipose tissues of rats with excess dietary intake
title_full_unstemmed Pilot study and bioinformatics analysis of differentially expressed genes in adipose tissues of rats with excess dietary intake
title_short Pilot study and bioinformatics analysis of differentially expressed genes in adipose tissues of rats with excess dietary intake
title_sort pilot study and bioinformatics analysis of differentially expressed genes in adipose tissues of rats with excess dietary intake
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115215/
https://www.ncbi.nlm.nih.gov/pubmed/32323762
http://dx.doi.org/10.3892/mmr.2020.11012
work_keys_str_mv AT yuanjunchao pilotstudyandbioinformaticsanalysisofdifferentiallyexpressedgenesinadiposetissuesofratswithexcessdietaryintake
AT yogarajahthaneswary pilotstudyandbioinformaticsanalysisofdifferentiallyexpressedgenesinadiposetissuesofratswithexcessdietaryintake
AT limshernkwok pilotstudyandbioinformaticsanalysisofdifferentiallyexpressedgenesinadiposetissuesofratswithexcessdietaryintake
AT teegetbeeyvonne pilotstudyandbioinformaticsanalysisofdifferentiallyexpressedgenesinadiposetissuesofratswithexcessdietaryintake
AT khooboonyin pilotstudyandbioinformaticsanalysisofdifferentiallyexpressedgenesinadiposetissuesofratswithexcessdietaryintake