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Anti-inflammatory role of Prunus persica L. Batsch methanol extract on lipopolysaccharide-stimulated glial cells

Glial cells are the resident immune cells of the central nervous system. Reactive glial cells release inflammatory mediators that induce neurotoxicity or aggravate neurodegeneration. Regulation of glial activation is crucial for the initiation and progression of neuropathological conditions. Constit...

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Autores principales: Seo, Kyoung Hee, Choi, So Young, Jin, Yeonsun, Son, Heebin, Kang, Young Sun, Jung, Seung Hyo, Kim, Yong-In, Eum, Sangmi, Bach, Tran The, Yoo, Hee Min, Whang, Wan Kyunn, Jung, Sun-Young, Kang, Wonku, Ko, Hyun Myung, Lee, Sung Hoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115241/
https://www.ncbi.nlm.nih.gov/pubmed/32186769
http://dx.doi.org/10.3892/mmr.2020.11016
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author Seo, Kyoung Hee
Choi, So Young
Jin, Yeonsun
Son, Heebin
Kang, Young Sun
Jung, Seung Hyo
Kim, Yong-In
Eum, Sangmi
Bach, Tran The
Yoo, Hee Min
Whang, Wan Kyunn
Jung, Sun-Young
Kang, Wonku
Ko, Hyun Myung
Lee, Sung Hoon
author_facet Seo, Kyoung Hee
Choi, So Young
Jin, Yeonsun
Son, Heebin
Kang, Young Sun
Jung, Seung Hyo
Kim, Yong-In
Eum, Sangmi
Bach, Tran The
Yoo, Hee Min
Whang, Wan Kyunn
Jung, Sun-Young
Kang, Wonku
Ko, Hyun Myung
Lee, Sung Hoon
author_sort Seo, Kyoung Hee
collection PubMed
description Glial cells are the resident immune cells of the central nervous system. Reactive glial cells release inflammatory mediators that induce neurotoxicity or aggravate neurodegeneration. Regulation of glial activation is crucial for the initiation and progression of neuropathological conditions. Constituents of the peach tree (Prunus persica L. Batsch), which has a global distribution, have been found to exert therapeutic effects in pathological conditions, such as rashes, eczema and allergies. However, the therapeutic potential of its aerial parts (leaves, fruits and twigs) remains to be elucidated. The present study aimed to evaluate the anti-inflammatory role of P. persica methanol extract (PPB) on lipopolysaccharide (LPS)-stimulated glial cells. High-performance liquid chromatography coupled with tandem mass spectrometry analysis showed that PPB contained chlorogenic acid and catechin, which have antioxidant properties. Western blot and reverse transcription polymerase chain reaction results indicated that PPB reduced the transcription of various proinflammatory enzymes (nitric oxide synthase and cyclooxygenase-2) and cytokines [tumor necrosis factor-α, interleukin (IL)-1β and IL-6] in LPS-stimulated BV2 cells. In addition, PPB inhibited the activation of NF-κB and various mitogen-activated protein kinases required for proinflammatory mediator transcription. Finally, nitrite measurement and immunocytochemistry results indicated that PPB also suppressed nitrite production and NF-κB translocation in LPS-stimulated primary astrocytes. Thus, PPB may be used as a potential therapeutic agent for neurodegenerative diseases and neurotoxicity via the suppression of glial cell activation.
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spelling pubmed-71152412020-04-08 Anti-inflammatory role of Prunus persica L. Batsch methanol extract on lipopolysaccharide-stimulated glial cells Seo, Kyoung Hee Choi, So Young Jin, Yeonsun Son, Heebin Kang, Young Sun Jung, Seung Hyo Kim, Yong-In Eum, Sangmi Bach, Tran The Yoo, Hee Min Whang, Wan Kyunn Jung, Sun-Young Kang, Wonku Ko, Hyun Myung Lee, Sung Hoon Mol Med Rep Articles Glial cells are the resident immune cells of the central nervous system. Reactive glial cells release inflammatory mediators that induce neurotoxicity or aggravate neurodegeneration. Regulation of glial activation is crucial for the initiation and progression of neuropathological conditions. Constituents of the peach tree (Prunus persica L. Batsch), which has a global distribution, have been found to exert therapeutic effects in pathological conditions, such as rashes, eczema and allergies. However, the therapeutic potential of its aerial parts (leaves, fruits and twigs) remains to be elucidated. The present study aimed to evaluate the anti-inflammatory role of P. persica methanol extract (PPB) on lipopolysaccharide (LPS)-stimulated glial cells. High-performance liquid chromatography coupled with tandem mass spectrometry analysis showed that PPB contained chlorogenic acid and catechin, which have antioxidant properties. Western blot and reverse transcription polymerase chain reaction results indicated that PPB reduced the transcription of various proinflammatory enzymes (nitric oxide synthase and cyclooxygenase-2) and cytokines [tumor necrosis factor-α, interleukin (IL)-1β and IL-6] in LPS-stimulated BV2 cells. In addition, PPB inhibited the activation of NF-κB and various mitogen-activated protein kinases required for proinflammatory mediator transcription. Finally, nitrite measurement and immunocytochemistry results indicated that PPB also suppressed nitrite production and NF-κB translocation in LPS-stimulated primary astrocytes. Thus, PPB may be used as a potential therapeutic agent for neurodegenerative diseases and neurotoxicity via the suppression of glial cell activation. D.A. Spandidos 2020-05 2020-03-10 /pmc/articles/PMC7115241/ /pubmed/32186769 http://dx.doi.org/10.3892/mmr.2020.11016 Text en Copyright: © Seo et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Seo, Kyoung Hee
Choi, So Young
Jin, Yeonsun
Son, Heebin
Kang, Young Sun
Jung, Seung Hyo
Kim, Yong-In
Eum, Sangmi
Bach, Tran The
Yoo, Hee Min
Whang, Wan Kyunn
Jung, Sun-Young
Kang, Wonku
Ko, Hyun Myung
Lee, Sung Hoon
Anti-inflammatory role of Prunus persica L. Batsch methanol extract on lipopolysaccharide-stimulated glial cells
title Anti-inflammatory role of Prunus persica L. Batsch methanol extract on lipopolysaccharide-stimulated glial cells
title_full Anti-inflammatory role of Prunus persica L. Batsch methanol extract on lipopolysaccharide-stimulated glial cells
title_fullStr Anti-inflammatory role of Prunus persica L. Batsch methanol extract on lipopolysaccharide-stimulated glial cells
title_full_unstemmed Anti-inflammatory role of Prunus persica L. Batsch methanol extract on lipopolysaccharide-stimulated glial cells
title_short Anti-inflammatory role of Prunus persica L. Batsch methanol extract on lipopolysaccharide-stimulated glial cells
title_sort anti-inflammatory role of prunus persica l. batsch methanol extract on lipopolysaccharide-stimulated glial cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115241/
https://www.ncbi.nlm.nih.gov/pubmed/32186769
http://dx.doi.org/10.3892/mmr.2020.11016
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