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ANKHD1 promotes proliferation and invasion of non-small-cell lung cancer cells via regulating YAP oncoprotein expression and inactivating the Hippo pathway

The ankyrin repeat and KH domain-containing 1 (ANKHD1) protein was recently reported to be a potential member of the Hippo signaling pathway. However, its role in human non-small-cell lung cancer (NSCLC) has not been extensively investigated. The aim of the present study was to examine the expressio...

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Autores principales: Liu, Xiao-Fang, Han, Qiang, Rong, Xue-Zhu, Yang, Man, Han, Yu-Chen, Yu, Juan-Han, Lin, Xu-Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115354/
https://www.ncbi.nlm.nih.gov/pubmed/32319569
http://dx.doi.org/10.3892/ijo.2020.4994
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author Liu, Xiao-Fang
Han, Qiang
Rong, Xue-Zhu
Yang, Man
Han, Yu-Chen
Yu, Juan-Han
Lin, Xu-Yong
author_facet Liu, Xiao-Fang
Han, Qiang
Rong, Xue-Zhu
Yang, Man
Han, Yu-Chen
Yu, Juan-Han
Lin, Xu-Yong
author_sort Liu, Xiao-Fang
collection PubMed
description The ankyrin repeat and KH domain-containing 1 (ANKHD1) protein was recently reported to be a potential member of the Hippo signaling pathway. However, its role in human non-small-cell lung cancer (NSCLC) has not been extensively investigated. The aim of the present study was to examine the expression of ANKHD1 in primary human tissues and cells and determine whether it is correlated with the clinical characteristics of tumor growth. The biological functions of ANKHD1 were evaluated in vitro and in vivo. Yes-associated protein (YAP) expression and phosphorylation induced by ANKHD1 were evaluated by western blotting and immunoprecipitation. Marked upregulation of ANKHD1 protein expression was observed in NSCLC cells and tissues, which was associated with advanced pathological tumor-node-metastasis stage, lymph node metastasis and poor prognosis in patients with NSCLC. ANKHD1 overexpression also promoted the proliferation and invasion of NSCLC cells. ANKHD1 upregulation inactivated Hippo signaling via increasing YAP protein levels, as well as inhibiting YAP protein phosphorylation, whereas depletion of YAP abolished the effects of ANKHD1 on cell proliferation and invasion. Therefore, ANKHD1 may play an important role in NSCLC through regulating the YAP-dependent Hippo signaling pathway.
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spelling pubmed-71153542020-04-08 ANKHD1 promotes proliferation and invasion of non-small-cell lung cancer cells via regulating YAP oncoprotein expression and inactivating the Hippo pathway Liu, Xiao-Fang Han, Qiang Rong, Xue-Zhu Yang, Man Han, Yu-Chen Yu, Juan-Han Lin, Xu-Yong Int J Oncol Articles The ankyrin repeat and KH domain-containing 1 (ANKHD1) protein was recently reported to be a potential member of the Hippo signaling pathway. However, its role in human non-small-cell lung cancer (NSCLC) has not been extensively investigated. The aim of the present study was to examine the expression of ANKHD1 in primary human tissues and cells and determine whether it is correlated with the clinical characteristics of tumor growth. The biological functions of ANKHD1 were evaluated in vitro and in vivo. Yes-associated protein (YAP) expression and phosphorylation induced by ANKHD1 were evaluated by western blotting and immunoprecipitation. Marked upregulation of ANKHD1 protein expression was observed in NSCLC cells and tissues, which was associated with advanced pathological tumor-node-metastasis stage, lymph node metastasis and poor prognosis in patients with NSCLC. ANKHD1 overexpression also promoted the proliferation and invasion of NSCLC cells. ANKHD1 upregulation inactivated Hippo signaling via increasing YAP protein levels, as well as inhibiting YAP protein phosphorylation, whereas depletion of YAP abolished the effects of ANKHD1 on cell proliferation and invasion. Therefore, ANKHD1 may play an important role in NSCLC through regulating the YAP-dependent Hippo signaling pathway. D.A. Spandidos 2020-02-19 /pmc/articles/PMC7115354/ /pubmed/32319569 http://dx.doi.org/10.3892/ijo.2020.4994 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Liu, Xiao-Fang
Han, Qiang
Rong, Xue-Zhu
Yang, Man
Han, Yu-Chen
Yu, Juan-Han
Lin, Xu-Yong
ANKHD1 promotes proliferation and invasion of non-small-cell lung cancer cells via regulating YAP oncoprotein expression and inactivating the Hippo pathway
title ANKHD1 promotes proliferation and invasion of non-small-cell lung cancer cells via regulating YAP oncoprotein expression and inactivating the Hippo pathway
title_full ANKHD1 promotes proliferation and invasion of non-small-cell lung cancer cells via regulating YAP oncoprotein expression and inactivating the Hippo pathway
title_fullStr ANKHD1 promotes proliferation and invasion of non-small-cell lung cancer cells via regulating YAP oncoprotein expression and inactivating the Hippo pathway
title_full_unstemmed ANKHD1 promotes proliferation and invasion of non-small-cell lung cancer cells via regulating YAP oncoprotein expression and inactivating the Hippo pathway
title_short ANKHD1 promotes proliferation and invasion of non-small-cell lung cancer cells via regulating YAP oncoprotein expression and inactivating the Hippo pathway
title_sort ankhd1 promotes proliferation and invasion of non-small-cell lung cancer cells via regulating yap oncoprotein expression and inactivating the hippo pathway
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115354/
https://www.ncbi.nlm.nih.gov/pubmed/32319569
http://dx.doi.org/10.3892/ijo.2020.4994
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