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Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) induced local and serum antibodies in mice
SARS-CoV (severe acute respiratory syndrome-associated coronavirus) strain GZ50 was partially purified and inactivated with 1:2000 formaldehyde. In cell culture the inactivated virus blocked the replication of live virus by decreasing the TCID(5.0) of the live virus 10(3.6) to 10(4.6) times. Inactiv...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Ltd.
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115388/ https://www.ncbi.nlm.nih.gov/pubmed/15603894 http://dx.doi.org/10.1016/j.vaccine.2004.07.031 |
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author | Qu, Di Zheng, Bojian Yao, Xin Guan, Yi Yuan, Zheng-Hong Zhong, Nan-Shan Lu, Li-Wei Xie, Jian-Ping Wen, Yu-Mei |
author_facet | Qu, Di Zheng, Bojian Yao, Xin Guan, Yi Yuan, Zheng-Hong Zhong, Nan-Shan Lu, Li-Wei Xie, Jian-Ping Wen, Yu-Mei |
author_sort | Qu, Di |
collection | PubMed |
description | SARS-CoV (severe acute respiratory syndrome-associated coronavirus) strain GZ50 was partially purified and inactivated with 1:2000 formaldehyde. In cell culture the inactivated virus blocked the replication of live virus by decreasing the TCID(5.0) of the live virus 10(3.6) to 10(4.6) times. Inactivated GZ50 was used to immunize mice intranasally either alone, or after precipitation with polyethylene glycol (PEG), or with CpG, or CTB as an adjuvant. The titer of serum neutralizing antibodies was up to 1:640. In mice immunized with adjuvants or PEG precipitated GZ50, specific IgA was detected in tracheal-lung wash fluid by immunofluorescence. Though serum antibodies were detected, no anti-SARS-IgA could be detected in mice immunized only with inactivated GZ50. The roles of adjuvants in intranasal immunization with inactivated. SARS-CoV is discussed. |
format | Online Article Text |
id | pubmed-7115388 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | Elsevier Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71153882020-04-02 Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) induced local and serum antibodies in mice Qu, Di Zheng, Bojian Yao, Xin Guan, Yi Yuan, Zheng-Hong Zhong, Nan-Shan Lu, Li-Wei Xie, Jian-Ping Wen, Yu-Mei Vaccine Article SARS-CoV (severe acute respiratory syndrome-associated coronavirus) strain GZ50 was partially purified and inactivated with 1:2000 formaldehyde. In cell culture the inactivated virus blocked the replication of live virus by decreasing the TCID(5.0) of the live virus 10(3.6) to 10(4.6) times. Inactivated GZ50 was used to immunize mice intranasally either alone, or after precipitation with polyethylene glycol (PEG), or with CpG, or CTB as an adjuvant. The titer of serum neutralizing antibodies was up to 1:640. In mice immunized with adjuvants or PEG precipitated GZ50, specific IgA was detected in tracheal-lung wash fluid by immunofluorescence. Though serum antibodies were detected, no anti-SARS-IgA could be detected in mice immunized only with inactivated GZ50. The roles of adjuvants in intranasal immunization with inactivated. SARS-CoV is discussed. Elsevier Ltd. 2005-01-04 2004-08-27 /pmc/articles/PMC7115388/ /pubmed/15603894 http://dx.doi.org/10.1016/j.vaccine.2004.07.031 Text en Copyright © 2004 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Qu, Di Zheng, Bojian Yao, Xin Guan, Yi Yuan, Zheng-Hong Zhong, Nan-Shan Lu, Li-Wei Xie, Jian-Ping Wen, Yu-Mei Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) induced local and serum antibodies in mice |
title | Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) induced local and serum antibodies in mice |
title_full | Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) induced local and serum antibodies in mice |
title_fullStr | Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) induced local and serum antibodies in mice |
title_full_unstemmed | Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) induced local and serum antibodies in mice |
title_short | Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) induced local and serum antibodies in mice |
title_sort | intranasal immunization with inactivated sars-cov (sars-associated coronavirus) induced local and serum antibodies in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115388/ https://www.ncbi.nlm.nih.gov/pubmed/15603894 http://dx.doi.org/10.1016/j.vaccine.2004.07.031 |
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