A recombinant DNA and vaccinia virus prime–boost regimen induces potent long-term T-cell responses to HCV in BALB/c mice

To explore the best prime–boost regimen and evaluate the T-cellular response memory against HCV, we constructed two DNA vaccine candidates (pVRC-CE1E2 and pAAV-CE1E2) and two recombinant viruses (rTTV-E1E2 and rAAV-E1E2) and then assessed the immune response to different prime–boost patterns in BALB...

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Autores principales: Deng, Yao, Zhang, Ke, Tan, Wenjie, Wang, Yue, Chen, Hong, Wu, Xiaobing, Ruan, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115398/
https://www.ncbi.nlm.nih.gov/pubmed/19356609
http://dx.doi.org/10.1016/j.vaccine.2009.02.003
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author Deng, Yao
Zhang, Ke
Tan, Wenjie
Wang, Yue
Chen, Hong
Wu, Xiaobing
Ruan, Li
author_facet Deng, Yao
Zhang, Ke
Tan, Wenjie
Wang, Yue
Chen, Hong
Wu, Xiaobing
Ruan, Li
author_sort Deng, Yao
collection PubMed
description To explore the best prime–boost regimen and evaluate the T-cellular response memory against HCV, we constructed two DNA vaccine candidates (pVRC-CE1E2 and pAAV-CE1E2) and two recombinant viruses (rTTV-E1E2 and rAAV-E1E2) and then assessed the immune response to different prime–boost patterns in BALB/c mice. The rTTV-E1E2 boosted the immune response to HCV DNA vaccine prime significantly, and the inverted terminal repeat sequence harboring DNA construct PAAV-CE1E2 was the best prime agent in this study. Our study provides new information for both the prime–boost regimen and long-term T-cell response for HCV vaccine development.
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spelling pubmed-71153982020-04-02 A recombinant DNA and vaccinia virus prime–boost regimen induces potent long-term T-cell responses to HCV in BALB/c mice Deng, Yao Zhang, Ke Tan, Wenjie Wang, Yue Chen, Hong Wu, Xiaobing Ruan, Li Vaccine Article To explore the best prime–boost regimen and evaluate the T-cellular response memory against HCV, we constructed two DNA vaccine candidates (pVRC-CE1E2 and pAAV-CE1E2) and two recombinant viruses (rTTV-E1E2 and rAAV-E1E2) and then assessed the immune response to different prime–boost patterns in BALB/c mice. The rTTV-E1E2 boosted the immune response to HCV DNA vaccine prime significantly, and the inverted terminal repeat sequence harboring DNA construct PAAV-CE1E2 was the best prime agent in this study. Our study provides new information for both the prime–boost regimen and long-term T-cell response for HCV vaccine development. Elsevier Ltd. 2009-03-26 2009-02-12 /pmc/articles/PMC7115398/ /pubmed/19356609 http://dx.doi.org/10.1016/j.vaccine.2009.02.003 Text en Copyright © 2009 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Deng, Yao
Zhang, Ke
Tan, Wenjie
Wang, Yue
Chen, Hong
Wu, Xiaobing
Ruan, Li
A recombinant DNA and vaccinia virus prime–boost regimen induces potent long-term T-cell responses to HCV in BALB/c mice
title A recombinant DNA and vaccinia virus prime–boost regimen induces potent long-term T-cell responses to HCV in BALB/c mice
title_full A recombinant DNA and vaccinia virus prime–boost regimen induces potent long-term T-cell responses to HCV in BALB/c mice
title_fullStr A recombinant DNA and vaccinia virus prime–boost regimen induces potent long-term T-cell responses to HCV in BALB/c mice
title_full_unstemmed A recombinant DNA and vaccinia virus prime–boost regimen induces potent long-term T-cell responses to HCV in BALB/c mice
title_short A recombinant DNA and vaccinia virus prime–boost regimen induces potent long-term T-cell responses to HCV in BALB/c mice
title_sort recombinant dna and vaccinia virus prime–boost regimen induces potent long-term t-cell responses to hcv in balb/c mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115398/
https://www.ncbi.nlm.nih.gov/pubmed/19356609
http://dx.doi.org/10.1016/j.vaccine.2009.02.003
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