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Preparation and characterization of SARS in-house reference antiserum

A reference antiserum for SARS is in urgent need in the development of SARS vaccine and other serological test of SARS research. Convalescent serum was collected from clinical confirmed patient. ELISA, Western-blotting and neutralization assay detected specific antibody against SARS coronavirus. Thi...

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Detalles Bibliográficos
Autores principales: Zhang, Jiansan, Liu, Yuxuan, Hu, Liangxiang, Gao, Qiang, Zhang, Zhenshan, Zhang, Xiaomei, Chen, Jiangting, Gong, Xuejie, Song, Lifei, Liu, Yufen, Li, Jing, Li, Shufen, Huang, Jinfeng, Ning, Ye, Gao, Hong, Qin, Chuan, Dong, Xiaoping, Wei, Jianguo, Dong, Guanmu, Yin, Weidong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115399/
https://www.ncbi.nlm.nih.gov/pubmed/16269206
http://dx.doi.org/10.1016/j.vaccine.2004.12.032
Descripción
Sumario:A reference antiserum for SARS is in urgent need in the development of SARS vaccine and other serological test of SARS research. Convalescent serum was collected from clinical confirmed patient. ELISA, Western-blotting and neutralization assay detected specific antibody against SARS coronavirus. This antiserum was prepared as in-house reference antiserum, freeze-dried and sealed in ampoules. The potency of this reference antiserum is defined to be 52.7 U after extensive calibration. Further, collaborative studies for the evaluation of this serum are needed in order to satisfy the requirements for international reference antiserum.