Preparation and characterization of SARS in-house reference antiserum

A reference antiserum for SARS is in urgent need in the development of SARS vaccine and other serological test of SARS research. Convalescent serum was collected from clinical confirmed patient. ELISA, Western-blotting and neutralization assay detected specific antibody against SARS coronavirus. Thi...

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Autores principales: Zhang, Jiansan, Liu, Yuxuan, Hu, Liangxiang, Gao, Qiang, Zhang, Zhenshan, Zhang, Xiaomei, Chen, Jiangting, Gong, Xuejie, Song, Lifei, Liu, Yufen, Li, Jing, Li, Shufen, Huang, Jinfeng, Ning, Ye, Gao, Hong, Qin, Chuan, Dong, Xiaoping, Wei, Jianguo, Dong, Guanmu, Yin, Weidong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2005
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115399/
https://www.ncbi.nlm.nih.gov/pubmed/16269206
http://dx.doi.org/10.1016/j.vaccine.2004.12.032
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author Zhang, Jiansan
Liu, Yuxuan
Hu, Liangxiang
Gao, Qiang
Zhang, Zhenshan
Zhang, Xiaomei
Chen, Jiangting
Gong, Xuejie
Song, Lifei
Liu, Yufen
Li, Jing
Li, Shufen
Huang, Jinfeng
Ning, Ye
Gao, Hong
Qin, Chuan
Dong, Xiaoping
Wei, Jianguo
Dong, Guanmu
Yin, Weidong
author_facet Zhang, Jiansan
Liu, Yuxuan
Hu, Liangxiang
Gao, Qiang
Zhang, Zhenshan
Zhang, Xiaomei
Chen, Jiangting
Gong, Xuejie
Song, Lifei
Liu, Yufen
Li, Jing
Li, Shufen
Huang, Jinfeng
Ning, Ye
Gao, Hong
Qin, Chuan
Dong, Xiaoping
Wei, Jianguo
Dong, Guanmu
Yin, Weidong
author_sort Zhang, Jiansan
collection PubMed
description A reference antiserum for SARS is in urgent need in the development of SARS vaccine and other serological test of SARS research. Convalescent serum was collected from clinical confirmed patient. ELISA, Western-blotting and neutralization assay detected specific antibody against SARS coronavirus. This antiserum was prepared as in-house reference antiserum, freeze-dried and sealed in ampoules. The potency of this reference antiserum is defined to be 52.7 U after extensive calibration. Further, collaborative studies for the evaluation of this serum are needed in order to satisfy the requirements for international reference antiserum.
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spelling pubmed-71153992020-04-02 Preparation and characterization of SARS in-house reference antiserum Zhang, Jiansan Liu, Yuxuan Hu, Liangxiang Gao, Qiang Zhang, Zhenshan Zhang, Xiaomei Chen, Jiangting Gong, Xuejie Song, Lifei Liu, Yufen Li, Jing Li, Shufen Huang, Jinfeng Ning, Ye Gao, Hong Qin, Chuan Dong, Xiaoping Wei, Jianguo Dong, Guanmu Yin, Weidong Vaccine Article A reference antiserum for SARS is in urgent need in the development of SARS vaccine and other serological test of SARS research. Convalescent serum was collected from clinical confirmed patient. ELISA, Western-blotting and neutralization assay detected specific antibody against SARS coronavirus. This antiserum was prepared as in-house reference antiserum, freeze-dried and sealed in ampoules. The potency of this reference antiserum is defined to be 52.7 U after extensive calibration. Further, collaborative studies for the evaluation of this serum are needed in order to satisfy the requirements for international reference antiserum. Elsevier Ltd. 2005-12-01 2005-03-16 /pmc/articles/PMC7115399/ /pubmed/16269206 http://dx.doi.org/10.1016/j.vaccine.2004.12.032 Text en Copyright © 2005 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Zhang, Jiansan
Liu, Yuxuan
Hu, Liangxiang
Gao, Qiang
Zhang, Zhenshan
Zhang, Xiaomei
Chen, Jiangting
Gong, Xuejie
Song, Lifei
Liu, Yufen
Li, Jing
Li, Shufen
Huang, Jinfeng
Ning, Ye
Gao, Hong
Qin, Chuan
Dong, Xiaoping
Wei, Jianguo
Dong, Guanmu
Yin, Weidong
Preparation and characterization of SARS in-house reference antiserum
title Preparation and characterization of SARS in-house reference antiserum
title_full Preparation and characterization of SARS in-house reference antiserum
title_fullStr Preparation and characterization of SARS in-house reference antiserum
title_full_unstemmed Preparation and characterization of SARS in-house reference antiserum
title_short Preparation and characterization of SARS in-house reference antiserum
title_sort preparation and characterization of sars in-house reference antiserum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115399/
https://www.ncbi.nlm.nih.gov/pubmed/16269206
http://dx.doi.org/10.1016/j.vaccine.2004.12.032
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