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Chimeric calicivirus-like particles elicit specific immune responses in pigs
Virus-like particles (VLPs) have received considerable attention due to their potential application in veterinary vaccines and, in particular, VLPs from rabbit haemorrhagic disease virus (RHDV) have successfully shown to be good platforms for inducing immune responses against an inserted foreign epi...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Ltd.
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115503/ https://www.ncbi.nlm.nih.gov/pubmed/22306796 http://dx.doi.org/10.1016/j.vaccine.2012.01.069 |
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author | Crisci, E. Fraile, L. Moreno, N. Blanco, E. Cabezón, R. Costa, C. Mussá, T. Baratelli, M. Martinez-Orellana, P. Ganges, L. Martínez, J. Bárcena, J. Montoya, M. |
author_facet | Crisci, E. Fraile, L. Moreno, N. Blanco, E. Cabezón, R. Costa, C. Mussá, T. Baratelli, M. Martinez-Orellana, P. Ganges, L. Martínez, J. Bárcena, J. Montoya, M. |
author_sort | Crisci, E. |
collection | PubMed |
description | Virus-like particles (VLPs) have received considerable attention due to their potential application in veterinary vaccines and, in particular, VLPs from rabbit haemorrhagic disease virus (RHDV) have successfully shown to be good platforms for inducing immune responses against an inserted foreign epitope in mice. The aim of this study was to assess the immunogenicity of chimeric RHDV-VLPs as vaccine vectors in pigs. For this purpose, we have generated chimeric VLPs containing a well-known T epitope of 3A protein of foot-and-mouth disease virus (FMDV). Firstly, RHDV-VLPs were able to activate immature porcine bone marrow-derived dendritic cells (poBMDCs) in vitro. Secondly, pigs were inoculated twice in a two-week interval with chimeric RHDV-VLPs at different doses intranasally or intramuscularly. One intramuscularly treated group was also inoculated with adjuvant Montanide™ ISA 206 at the same time. Specific IgG and IgA antibodies against RHDV-VLPs were induced and such levels were higher in the adjuvanted group compared with other groups. Interestingly, anti-RHDV-VLP IgA responses were higher in groups inoculated intramuscularly than those that received the VLPs intranasally. Two weeks after the last immunisation, specific IFN-γ-secreting cells against 3A epitope and against RHDV-VLPs were detected in PBMCs by ELISPOT. The adjuvanted group exhibited the highest IFN-γ-secreting cell numbers and lymphoproliferative specific T cell responses against 3A epitope and RHDV-VLP. This is the first immunological report on the potential use of chimeric RHDV-VLPs as antigen carriers in pigs. |
format | Online Article Text |
id | pubmed-7115503 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Elsevier Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71155032020-04-02 Chimeric calicivirus-like particles elicit specific immune responses in pigs Crisci, E. Fraile, L. Moreno, N. Blanco, E. Cabezón, R. Costa, C. Mussá, T. Baratelli, M. Martinez-Orellana, P. Ganges, L. Martínez, J. Bárcena, J. Montoya, M. Vaccine Article Virus-like particles (VLPs) have received considerable attention due to their potential application in veterinary vaccines and, in particular, VLPs from rabbit haemorrhagic disease virus (RHDV) have successfully shown to be good platforms for inducing immune responses against an inserted foreign epitope in mice. The aim of this study was to assess the immunogenicity of chimeric RHDV-VLPs as vaccine vectors in pigs. For this purpose, we have generated chimeric VLPs containing a well-known T epitope of 3A protein of foot-and-mouth disease virus (FMDV). Firstly, RHDV-VLPs were able to activate immature porcine bone marrow-derived dendritic cells (poBMDCs) in vitro. Secondly, pigs were inoculated twice in a two-week interval with chimeric RHDV-VLPs at different doses intranasally or intramuscularly. One intramuscularly treated group was also inoculated with adjuvant Montanide™ ISA 206 at the same time. Specific IgG and IgA antibodies against RHDV-VLPs were induced and such levels were higher in the adjuvanted group compared with other groups. Interestingly, anti-RHDV-VLP IgA responses were higher in groups inoculated intramuscularly than those that received the VLPs intranasally. Two weeks after the last immunisation, specific IFN-γ-secreting cells against 3A epitope and against RHDV-VLPs were detected in PBMCs by ELISPOT. The adjuvanted group exhibited the highest IFN-γ-secreting cell numbers and lymphoproliferative specific T cell responses against 3A epitope and RHDV-VLP. This is the first immunological report on the potential use of chimeric RHDV-VLPs as antigen carriers in pigs. Elsevier Ltd. 2012-03-23 2012-02-03 /pmc/articles/PMC7115503/ /pubmed/22306796 http://dx.doi.org/10.1016/j.vaccine.2012.01.069 Text en Copyright © 2012 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Crisci, E. Fraile, L. Moreno, N. Blanco, E. Cabezón, R. Costa, C. Mussá, T. Baratelli, M. Martinez-Orellana, P. Ganges, L. Martínez, J. Bárcena, J. Montoya, M. Chimeric calicivirus-like particles elicit specific immune responses in pigs |
title | Chimeric calicivirus-like particles elicit specific immune responses in pigs |
title_full | Chimeric calicivirus-like particles elicit specific immune responses in pigs |
title_fullStr | Chimeric calicivirus-like particles elicit specific immune responses in pigs |
title_full_unstemmed | Chimeric calicivirus-like particles elicit specific immune responses in pigs |
title_short | Chimeric calicivirus-like particles elicit specific immune responses in pigs |
title_sort | chimeric calicivirus-like particles elicit specific immune responses in pigs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115503/ https://www.ncbi.nlm.nih.gov/pubmed/22306796 http://dx.doi.org/10.1016/j.vaccine.2012.01.069 |
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