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A truncated fragment of Ov-ASP-1 consisting of the core pathogenesis-related-1 (PR-1) domain maintains adjuvanticity as the full-length protein

The Onchocerca volvulus activation-associated secreted protein-1 (Ov-ASP-1) has good adjuvanticity for a variety of antigens and vaccines, probably due to its ability activate antigen-processing cells (APCs). However, the functional domain of Ov-ASP-1 as an adjuvant is not clearly defined. Based on...

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Autores principales: Guo, Jingjing, Yang, Yi, Xiao, Wenjun, Sun, Weilai, Yu, Hong, Du, Lanying, Lustigman, Sara, Jiang, Shibo, Kou, Zhihua, Zhou, Yusen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115538/
https://www.ncbi.nlm.nih.gov/pubmed/25736195
http://dx.doi.org/10.1016/j.vaccine.2015.02.053
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author Guo, Jingjing
Yang, Yi
Xiao, Wenjun
Sun, Weilai
Yu, Hong
Du, Lanying
Lustigman, Sara
Jiang, Shibo
Kou, Zhihua
Zhou, Yusen
author_facet Guo, Jingjing
Yang, Yi
Xiao, Wenjun
Sun, Weilai
Yu, Hong
Du, Lanying
Lustigman, Sara
Jiang, Shibo
Kou, Zhihua
Zhou, Yusen
author_sort Guo, Jingjing
collection PubMed
description The Onchocerca volvulus activation-associated secreted protein-1 (Ov-ASP-1) has good adjuvanticity for a variety of antigens and vaccines, probably due to its ability activate antigen-processing cells (APCs). However, the functional domain of Ov-ASP-1 as an adjuvant is not clearly defined. Based on the structural prediction of this protein family, we constructed a 16-kDa recombinant protein of Ov-ASP-1 that contains only the core pathogenesis-related-1 (PR-1) domain (residues 10–153), designated ASPPR. We found that ASPPR exhibits adjuvanticity similar to that of the full-length Ov-ASP-1 (residues 10–220) for various antigens, including ovalbumin (OVA), HBsAg protein antigen, and the HIV peptide 5 (Pep5) antigen, but it is more suitable for vaccine design in ASPPR-antigen fusion proteins, and more stable in PBS than Ov-ASP-1 stored at −70 °C. These results suggest that ASPPR might be the functional region of Ov-ASP-1 as an adjuvant, and therefore could be developed as an adjuvant for human use.
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spelling pubmed-71155382020-04-02 A truncated fragment of Ov-ASP-1 consisting of the core pathogenesis-related-1 (PR-1) domain maintains adjuvanticity as the full-length protein Guo, Jingjing Yang, Yi Xiao, Wenjun Sun, Weilai Yu, Hong Du, Lanying Lustigman, Sara Jiang, Shibo Kou, Zhihua Zhou, Yusen Vaccine Article The Onchocerca volvulus activation-associated secreted protein-1 (Ov-ASP-1) has good adjuvanticity for a variety of antigens and vaccines, probably due to its ability activate antigen-processing cells (APCs). However, the functional domain of Ov-ASP-1 as an adjuvant is not clearly defined. Based on the structural prediction of this protein family, we constructed a 16-kDa recombinant protein of Ov-ASP-1 that contains only the core pathogenesis-related-1 (PR-1) domain (residues 10–153), designated ASPPR. We found that ASPPR exhibits adjuvanticity similar to that of the full-length Ov-ASP-1 (residues 10–220) for various antigens, including ovalbumin (OVA), HBsAg protein antigen, and the HIV peptide 5 (Pep5) antigen, but it is more suitable for vaccine design in ASPPR-antigen fusion proteins, and more stable in PBS than Ov-ASP-1 stored at −70 °C. These results suggest that ASPPR might be the functional region of Ov-ASP-1 as an adjuvant, and therefore could be developed as an adjuvant for human use. Elsevier Ltd. 2015-04-15 2015-02-28 /pmc/articles/PMC7115538/ /pubmed/25736195 http://dx.doi.org/10.1016/j.vaccine.2015.02.053 Text en Copyright © 2015 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Guo, Jingjing
Yang, Yi
Xiao, Wenjun
Sun, Weilai
Yu, Hong
Du, Lanying
Lustigman, Sara
Jiang, Shibo
Kou, Zhihua
Zhou, Yusen
A truncated fragment of Ov-ASP-1 consisting of the core pathogenesis-related-1 (PR-1) domain maintains adjuvanticity as the full-length protein
title A truncated fragment of Ov-ASP-1 consisting of the core pathogenesis-related-1 (PR-1) domain maintains adjuvanticity as the full-length protein
title_full A truncated fragment of Ov-ASP-1 consisting of the core pathogenesis-related-1 (PR-1) domain maintains adjuvanticity as the full-length protein
title_fullStr A truncated fragment of Ov-ASP-1 consisting of the core pathogenesis-related-1 (PR-1) domain maintains adjuvanticity as the full-length protein
title_full_unstemmed A truncated fragment of Ov-ASP-1 consisting of the core pathogenesis-related-1 (PR-1) domain maintains adjuvanticity as the full-length protein
title_short A truncated fragment of Ov-ASP-1 consisting of the core pathogenesis-related-1 (PR-1) domain maintains adjuvanticity as the full-length protein
title_sort truncated fragment of ov-asp-1 consisting of the core pathogenesis-related-1 (pr-1) domain maintains adjuvanticity as the full-length protein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115538/
https://www.ncbi.nlm.nih.gov/pubmed/25736195
http://dx.doi.org/10.1016/j.vaccine.2015.02.053
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