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Immune responses in Balb/c mice induced by a candidate SARS-CoV inactivated vaccine prepared from F69 strain

The immunogenicity of a candidate-inactivated vaccine prepared from SARS-CoV F69 strain was evaluated in Balb/c mice. Potent humoral immune responses were induced under the elicitation of three times of immunizations at 2-week intervals with this vaccine, combined with three types of adjuvants (Freu...

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Autores principales: Zhang, Chuan-hai, Lu, Jia-hai, Wang, Yi-fei, Zheng, Huan-ying, Xiong, Sheng, Zhang, Mei-ying, Liu, Xin-jian, Li, Jiu-xiang, Wan, Zhuo-yue, Yan, Xin-ge, Qi, Shu-Yuan, Cui, Zhiyong, Zhang, Biliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115646/
https://www.ncbi.nlm.nih.gov/pubmed/15837220
http://dx.doi.org/10.1016/j.vaccine.2004.11.073
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author Zhang, Chuan-hai
Lu, Jia-hai
Wang, Yi-fei
Zheng, Huan-ying
Xiong, Sheng
Zhang, Mei-ying
Liu, Xin-jian
Li, Jiu-xiang
Wan, Zhuo-yue
Yan, Xin-ge
Qi, Shu-Yuan
Cui, Zhiyong
Zhang, Biliang
author_facet Zhang, Chuan-hai
Lu, Jia-hai
Wang, Yi-fei
Zheng, Huan-ying
Xiong, Sheng
Zhang, Mei-ying
Liu, Xin-jian
Li, Jiu-xiang
Wan, Zhuo-yue
Yan, Xin-ge
Qi, Shu-Yuan
Cui, Zhiyong
Zhang, Biliang
author_sort Zhang, Chuan-hai
collection PubMed
description The immunogenicity of a candidate-inactivated vaccine prepared from SARS-CoV F69 strain was evaluated in Balb/c mice. Potent humoral immune responses were induced under the elicitation of three times of immunizations at 2-week intervals with this vaccine, combined with three types of adjuvants (Freund's adjuvant, Al(OH)(3) adjuvant and CpG adjuvant). Titers of specific IgG antibodies in three test groups all peaked in the sixth week after first vaccination, but significant differences existed in the kinetics of specific IgG antibody levels. The strong neutralizing capacity exhibited in micro-cytopathic effect neutralization tests indicated the specific antibodies are protective. Western blot assay further demonstrated the specificity of the induced serum antibodies.
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spelling pubmed-71156462020-04-02 Immune responses in Balb/c mice induced by a candidate SARS-CoV inactivated vaccine prepared from F69 strain Zhang, Chuan-hai Lu, Jia-hai Wang, Yi-fei Zheng, Huan-ying Xiong, Sheng Zhang, Mei-ying Liu, Xin-jian Li, Jiu-xiang Wan, Zhuo-yue Yan, Xin-ge Qi, Shu-Yuan Cui, Zhiyong Zhang, Biliang Vaccine Article The immunogenicity of a candidate-inactivated vaccine prepared from SARS-CoV F69 strain was evaluated in Balb/c mice. Potent humoral immune responses were induced under the elicitation of three times of immunizations at 2-week intervals with this vaccine, combined with three types of adjuvants (Freund's adjuvant, Al(OH)(3) adjuvant and CpG adjuvant). Titers of specific IgG antibodies in three test groups all peaked in the sixth week after first vaccination, but significant differences existed in the kinetics of specific IgG antibody levels. The strong neutralizing capacity exhibited in micro-cytopathic effect neutralization tests indicated the specific antibodies are protective. Western blot assay further demonstrated the specificity of the induced serum antibodies. Elsevier Ltd. 2005-05-02 2005-01-21 /pmc/articles/PMC7115646/ /pubmed/15837220 http://dx.doi.org/10.1016/j.vaccine.2004.11.073 Text en Copyright © 2005 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Zhang, Chuan-hai
Lu, Jia-hai
Wang, Yi-fei
Zheng, Huan-ying
Xiong, Sheng
Zhang, Mei-ying
Liu, Xin-jian
Li, Jiu-xiang
Wan, Zhuo-yue
Yan, Xin-ge
Qi, Shu-Yuan
Cui, Zhiyong
Zhang, Biliang
Immune responses in Balb/c mice induced by a candidate SARS-CoV inactivated vaccine prepared from F69 strain
title Immune responses in Balb/c mice induced by a candidate SARS-CoV inactivated vaccine prepared from F69 strain
title_full Immune responses in Balb/c mice induced by a candidate SARS-CoV inactivated vaccine prepared from F69 strain
title_fullStr Immune responses in Balb/c mice induced by a candidate SARS-CoV inactivated vaccine prepared from F69 strain
title_full_unstemmed Immune responses in Balb/c mice induced by a candidate SARS-CoV inactivated vaccine prepared from F69 strain
title_short Immune responses in Balb/c mice induced by a candidate SARS-CoV inactivated vaccine prepared from F69 strain
title_sort immune responses in balb/c mice induced by a candidate sars-cov inactivated vaccine prepared from f69 strain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115646/
https://www.ncbi.nlm.nih.gov/pubmed/15837220
http://dx.doi.org/10.1016/j.vaccine.2004.11.073
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