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Immune responses in Balb/c mice induced by a candidate SARS-CoV inactivated vaccine prepared from F69 strain
The immunogenicity of a candidate-inactivated vaccine prepared from SARS-CoV F69 strain was evaluated in Balb/c mice. Potent humoral immune responses were induced under the elicitation of three times of immunizations at 2-week intervals with this vaccine, combined with three types of adjuvants (Freu...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Ltd.
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115646/ https://www.ncbi.nlm.nih.gov/pubmed/15837220 http://dx.doi.org/10.1016/j.vaccine.2004.11.073 |
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author | Zhang, Chuan-hai Lu, Jia-hai Wang, Yi-fei Zheng, Huan-ying Xiong, Sheng Zhang, Mei-ying Liu, Xin-jian Li, Jiu-xiang Wan, Zhuo-yue Yan, Xin-ge Qi, Shu-Yuan Cui, Zhiyong Zhang, Biliang |
author_facet | Zhang, Chuan-hai Lu, Jia-hai Wang, Yi-fei Zheng, Huan-ying Xiong, Sheng Zhang, Mei-ying Liu, Xin-jian Li, Jiu-xiang Wan, Zhuo-yue Yan, Xin-ge Qi, Shu-Yuan Cui, Zhiyong Zhang, Biliang |
author_sort | Zhang, Chuan-hai |
collection | PubMed |
description | The immunogenicity of a candidate-inactivated vaccine prepared from SARS-CoV F69 strain was evaluated in Balb/c mice. Potent humoral immune responses were induced under the elicitation of three times of immunizations at 2-week intervals with this vaccine, combined with three types of adjuvants (Freund's adjuvant, Al(OH)(3) adjuvant and CpG adjuvant). Titers of specific IgG antibodies in three test groups all peaked in the sixth week after first vaccination, but significant differences existed in the kinetics of specific IgG antibody levels. The strong neutralizing capacity exhibited in micro-cytopathic effect neutralization tests indicated the specific antibodies are protective. Western blot assay further demonstrated the specificity of the induced serum antibodies. |
format | Online Article Text |
id | pubmed-7115646 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | Elsevier Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71156462020-04-02 Immune responses in Balb/c mice induced by a candidate SARS-CoV inactivated vaccine prepared from F69 strain Zhang, Chuan-hai Lu, Jia-hai Wang, Yi-fei Zheng, Huan-ying Xiong, Sheng Zhang, Mei-ying Liu, Xin-jian Li, Jiu-xiang Wan, Zhuo-yue Yan, Xin-ge Qi, Shu-Yuan Cui, Zhiyong Zhang, Biliang Vaccine Article The immunogenicity of a candidate-inactivated vaccine prepared from SARS-CoV F69 strain was evaluated in Balb/c mice. Potent humoral immune responses were induced under the elicitation of three times of immunizations at 2-week intervals with this vaccine, combined with three types of adjuvants (Freund's adjuvant, Al(OH)(3) adjuvant and CpG adjuvant). Titers of specific IgG antibodies in three test groups all peaked in the sixth week after first vaccination, but significant differences existed in the kinetics of specific IgG antibody levels. The strong neutralizing capacity exhibited in micro-cytopathic effect neutralization tests indicated the specific antibodies are protective. Western blot assay further demonstrated the specificity of the induced serum antibodies. Elsevier Ltd. 2005-05-02 2005-01-21 /pmc/articles/PMC7115646/ /pubmed/15837220 http://dx.doi.org/10.1016/j.vaccine.2004.11.073 Text en Copyright © 2005 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Zhang, Chuan-hai Lu, Jia-hai Wang, Yi-fei Zheng, Huan-ying Xiong, Sheng Zhang, Mei-ying Liu, Xin-jian Li, Jiu-xiang Wan, Zhuo-yue Yan, Xin-ge Qi, Shu-Yuan Cui, Zhiyong Zhang, Biliang Immune responses in Balb/c mice induced by a candidate SARS-CoV inactivated vaccine prepared from F69 strain |
title | Immune responses in Balb/c mice induced by a candidate SARS-CoV inactivated vaccine prepared from F69 strain |
title_full | Immune responses in Balb/c mice induced by a candidate SARS-CoV inactivated vaccine prepared from F69 strain |
title_fullStr | Immune responses in Balb/c mice induced by a candidate SARS-CoV inactivated vaccine prepared from F69 strain |
title_full_unstemmed | Immune responses in Balb/c mice induced by a candidate SARS-CoV inactivated vaccine prepared from F69 strain |
title_short | Immune responses in Balb/c mice induced by a candidate SARS-CoV inactivated vaccine prepared from F69 strain |
title_sort | immune responses in balb/c mice induced by a candidate sars-cov inactivated vaccine prepared from f69 strain |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115646/ https://www.ncbi.nlm.nih.gov/pubmed/15837220 http://dx.doi.org/10.1016/j.vaccine.2004.11.073 |
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