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A novel and exploitable antifungal peptide from kale (Brassica alboglabra) seeds
The aim of this study was to purify and characterize antifungal peptides from kale seeds in view of the paucity of information on antifungal peptides from the family Brassicaceae, and to compare its characteristics with those of published Brassica antifungal peptides. A 5907-Da antifungal peptide wa...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Inc.
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115674/ https://www.ncbi.nlm.nih.gov/pubmed/18597893 http://dx.doi.org/10.1016/j.peptides.2008.05.020 |
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author | Lin, Peng Ng, Tzi Bun |
author_facet | Lin, Peng Ng, Tzi Bun |
author_sort | Lin, Peng |
collection | PubMed |
description | The aim of this study was to purify and characterize antifungal peptides from kale seeds in view of the paucity of information on antifungal peptides from the family Brassicaceae, and to compare its characteristics with those of published Brassica antifungal peptides. A 5907-Da antifungal peptide was isolated from kale seeds. The isolation procedure comprised affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-Sepharose and Mono S, and gel filtration on Superdex Peptide. The peptide was adsorbed on the first three chromatographic media. It inhibited mycelial growth in a number of fungal species including Fusarium oxysporum, Helminthosporium maydis, Mycosphaerella arachidicola and Valsa mali, with an IC(50) of 4.3 μM, 2.1 μM, 2.4 μM, and 0.15 μM, respectively and exhibited pronounced thermostability and pH stability. It inhibited proliferation of hepatoma (HepG2) and breast cancer (MCF7) cells with an IC(50) of 2.7 μM and 3.4 μM, and the activity of HIV-1 reverse transcriptase with an IC(50) of 4.9 μM. Its N-terminal sequence differed from those of antifungal proteins which have been reported to date. |
format | Online Article Text |
id | pubmed-7115674 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71156742020-04-02 A novel and exploitable antifungal peptide from kale (Brassica alboglabra) seeds Lin, Peng Ng, Tzi Bun Peptides Article The aim of this study was to purify and characterize antifungal peptides from kale seeds in view of the paucity of information on antifungal peptides from the family Brassicaceae, and to compare its characteristics with those of published Brassica antifungal peptides. A 5907-Da antifungal peptide was isolated from kale seeds. The isolation procedure comprised affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-Sepharose and Mono S, and gel filtration on Superdex Peptide. The peptide was adsorbed on the first three chromatographic media. It inhibited mycelial growth in a number of fungal species including Fusarium oxysporum, Helminthosporium maydis, Mycosphaerella arachidicola and Valsa mali, with an IC(50) of 4.3 μM, 2.1 μM, 2.4 μM, and 0.15 μM, respectively and exhibited pronounced thermostability and pH stability. It inhibited proliferation of hepatoma (HepG2) and breast cancer (MCF7) cells with an IC(50) of 2.7 μM and 3.4 μM, and the activity of HIV-1 reverse transcriptase with an IC(50) of 4.9 μM. Its N-terminal sequence differed from those of antifungal proteins which have been reported to date. Elsevier Inc. 2008-10 2008-05-30 /pmc/articles/PMC7115674/ /pubmed/18597893 http://dx.doi.org/10.1016/j.peptides.2008.05.020 Text en Copyright © 2008 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Lin, Peng Ng, Tzi Bun A novel and exploitable antifungal peptide from kale (Brassica alboglabra) seeds |
title | A novel and exploitable antifungal peptide from kale (Brassica alboglabra) seeds |
title_full | A novel and exploitable antifungal peptide from kale (Brassica alboglabra) seeds |
title_fullStr | A novel and exploitable antifungal peptide from kale (Brassica alboglabra) seeds |
title_full_unstemmed | A novel and exploitable antifungal peptide from kale (Brassica alboglabra) seeds |
title_short | A novel and exploitable antifungal peptide from kale (Brassica alboglabra) seeds |
title_sort | novel and exploitable antifungal peptide from kale (brassica alboglabra) seeds |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115674/ https://www.ncbi.nlm.nih.gov/pubmed/18597893 http://dx.doi.org/10.1016/j.peptides.2008.05.020 |
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