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Detection of feline coronavirus using microcantilever sensors

This work demonstrated the feasibility of detecting severe acute respiratory syndrome associated coronavirus (SARS-CoV) using microcantilever technology by showing that the feline coronavirus (FIP) type I virus can be detected by a microcantilever modified by feline coronavirus (FIP) type I anti-vir...

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Detalles Bibliográficos
Autores principales: Velanki, Sreepriya, Ji, Hai-Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Institute of Physics 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115745/
https://www.ncbi.nlm.nih.gov/pubmed/32288326
http://dx.doi.org/10.1088/0957-0233/17/11/015
Descripción
Sumario:This work demonstrated the feasibility of detecting severe acute respiratory syndrome associated coronavirus (SARS-CoV) using microcantilever technology by showing that the feline coronavirus (FIP) type I virus can be detected by a microcantilever modified by feline coronavirus (FIP) type I anti-viral antiserum. A microcantilever modified by FIP type I anti-viral antiserum was developed for the detection of FIP type I virus. When the FIP type I virus positive sample is injected into the fluid cell where the microcantilever is held, the microcantilever bends upon the recognition of the FIP type I virus by the antiserum on the surface of the microcantilever. A negative control sample that does not contain FIP type I virus did not cause any bending of the microcantilever. The detection limit of the sensor was 0.1 µg ml(−1) when the assay time was <1 h.