Molecular assay for the detection of Cochlosoma anatis in house flies and turkey specimens by polymerase chain reaction

A 1520 bp region of Cochlosoma anatis mtDNA 16S gene was subjected to DNA sequencing and a 466 bp portion was compared with other protozoan 16S sequences to develop PCR primers specific for C. anatis. This PCR diagnostic method allowed identification of C. anatis from house flies, Musca domestica L....

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Detalles Bibliográficos
Autores principales: McElroy, Sheri M., Szalanski, Allen L., McKay, Tanja, Bermudez, Alex J., Owens, Carrie B., Steelman, C. Dayton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2005
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115810/
https://www.ncbi.nlm.nih.gov/pubmed/15631910
http://dx.doi.org/10.1016/j.vetpar.2004.09.015
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author McElroy, Sheri M.
Szalanski, Allen L.
McKay, Tanja
Bermudez, Alex J.
Owens, Carrie B.
Steelman, C. Dayton
author_facet McElroy, Sheri M.
Szalanski, Allen L.
McKay, Tanja
Bermudez, Alex J.
Owens, Carrie B.
Steelman, C. Dayton
author_sort McElroy, Sheri M.
collection PubMed
description A 1520 bp region of Cochlosoma anatis mtDNA 16S gene was subjected to DNA sequencing and a 466 bp portion was compared with other protozoan 16S sequences to develop PCR primers specific for C. anatis. This PCR diagnostic method allowed identification of C. anatis from house flies, Musca domestica L., turkey gut, and fecal samples within 6 h after field-collected samples reached the laboratory. House flies detected carrying C. anatis using the diagnostic 374 bp amplicons represented the first record of this protozoan in house flies.
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spelling pubmed-71158102020-04-02 Molecular assay for the detection of Cochlosoma anatis in house flies and turkey specimens by polymerase chain reaction McElroy, Sheri M. Szalanski, Allen L. McKay, Tanja Bermudez, Alex J. Owens, Carrie B. Steelman, C. Dayton Vet Parasitol Short Communication A 1520 bp region of Cochlosoma anatis mtDNA 16S gene was subjected to DNA sequencing and a 466 bp portion was compared with other protozoan 16S sequences to develop PCR primers specific for C. anatis. This PCR diagnostic method allowed identification of C. anatis from house flies, Musca domestica L., turkey gut, and fecal samples within 6 h after field-collected samples reached the laboratory. House flies detected carrying C. anatis using the diagnostic 374 bp amplicons represented the first record of this protozoan in house flies. Elsevier B.V. 2005-01-20 2004-11-11 /pmc/articles/PMC7115810/ /pubmed/15631910 http://dx.doi.org/10.1016/j.vetpar.2004.09.015 Text en Copyright © 2004 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Short Communication
McElroy, Sheri M.
Szalanski, Allen L.
McKay, Tanja
Bermudez, Alex J.
Owens, Carrie B.
Steelman, C. Dayton
Molecular assay for the detection of Cochlosoma anatis in house flies and turkey specimens by polymerase chain reaction
title Molecular assay for the detection of Cochlosoma anatis in house flies and turkey specimens by polymerase chain reaction
title_full Molecular assay for the detection of Cochlosoma anatis in house flies and turkey specimens by polymerase chain reaction
title_fullStr Molecular assay for the detection of Cochlosoma anatis in house flies and turkey specimens by polymerase chain reaction
title_full_unstemmed Molecular assay for the detection of Cochlosoma anatis in house flies and turkey specimens by polymerase chain reaction
title_short Molecular assay for the detection of Cochlosoma anatis in house flies and turkey specimens by polymerase chain reaction
title_sort molecular assay for the detection of cochlosoma anatis in house flies and turkey specimens by polymerase chain reaction
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115810/
https://www.ncbi.nlm.nih.gov/pubmed/15631910
http://dx.doi.org/10.1016/j.vetpar.2004.09.015
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