An efficient and novel technology for the extraction of parasite genomic DNA from whole blood or culture

The aim of this study was to assess pathogen DNA extraction with a new spin column-based method (DNA-XT). DNA from either whole-blood samples spiked with Plasmodium falciparum or Leishmania donovani amastigote culture was extracted with DNA-XT and compared with that produced by a commercial extracti...

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Detalles Bibliográficos
Autores principales: Clark, David J, Moore, Catherine M, Flanagan, Marc, Bocxlaer, Katrien Van, Piperaki, Evangelia-Theophano, Yardley, Vanessa, Croft, Simon L, Tyson, John, Whitehouse, Sam P, O’Halloran, Jonathan, Krishna, Sanjeev, Staines, Henry M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2019
Materias:
Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115988/
https://www.ncbi.nlm.nih.gov/pubmed/31774301
http://dx.doi.org/10.2144/btn-2019-0086
Descripción
Sumario:The aim of this study was to assess pathogen DNA extraction with a new spin column-based method (DNA-XT). DNA from either whole-blood samples spiked with Plasmodium falciparum or Leishmania donovani amastigote culture was extracted with DNA-XT and compared with that produced by a commercial extraction kit (DNeasy(®)). Eluates from large and small sample volumes were assessed by PCR and spectroscopy. Using a small volume (5 μl) of blood, the DNA-XT and DNeasy methods produced eluates with similar DNA concentrations (0.63 vs 1.06 ng/μl, respectively). The DNA-XT method produced DNA with lower PCR inhibition than DNeasy. The new technique was also twice as fast and required fewer plastics and manipulations but had reduced total recovered DNA compared with DNeasy.

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