Ordered Lipid Domains Assemble via Concerted Recruitment of Constituents from Both Membrane Leaflets

Lipid rafts serve as anchoring platforms for membrane proteins. Thus far they escaped direct observation by light microscopy due to their small size. Here we used differently colored dyes as reporters for the registration of both ordered and disordered lipids from the two leaves of a freestanding bi...

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Autores principales: Saitov, Ali, Akimov, Sergey A., Galimzyanov, Timur R., Glasnov, Toma, Pohl, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115998/
https://www.ncbi.nlm.nih.gov/pubmed/32216409
http://dx.doi.org/10.1103/PhysRevLett.124.108102
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author Saitov, Ali
Akimov, Sergey A.
Galimzyanov, Timur R.
Glasnov, Toma
Pohl, Peter
author_facet Saitov, Ali
Akimov, Sergey A.
Galimzyanov, Timur R.
Glasnov, Toma
Pohl, Peter
author_sort Saitov, Ali
collection PubMed
description Lipid rafts serve as anchoring platforms for membrane proteins. Thus far they escaped direct observation by light microscopy due to their small size. Here we used differently colored dyes as reporters for the registration of both ordered and disordered lipids from the two leaves of a freestanding bilayer. Photoswitchable lipids dissolved or reformed the domains. Measurements of domain mobility indicated the presence of 120 nm wide ordered and 40 nm wide disordered domains. These sizes are in line with the predicted roles of line tension and membrane undulation as driving forces for alignment.
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spelling pubmed-71159982020-09-01 Ordered Lipid Domains Assemble via Concerted Recruitment of Constituents from Both Membrane Leaflets Saitov, Ali Akimov, Sergey A. Galimzyanov, Timur R. Glasnov, Toma Pohl, Peter Phys Rev Lett Article Lipid rafts serve as anchoring platforms for membrane proteins. Thus far they escaped direct observation by light microscopy due to their small size. Here we used differently colored dyes as reporters for the registration of both ordered and disordered lipids from the two leaves of a freestanding bilayer. Photoswitchable lipids dissolved or reformed the domains. Measurements of domain mobility indicated the presence of 120 nm wide ordered and 40 nm wide disordered domains. These sizes are in line with the predicted roles of line tension and membrane undulation as driving forces for alignment. 2020-03-13 /pmc/articles/PMC7115998/ /pubmed/32216409 http://dx.doi.org/10.1103/PhysRevLett.124.108102 Text en https://creativecommons.org/licenses/by/4.0/ Published by the American Physical Society under the terms of the Creative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/) license.
spellingShingle Article
Saitov, Ali
Akimov, Sergey A.
Galimzyanov, Timur R.
Glasnov, Toma
Pohl, Peter
Ordered Lipid Domains Assemble via Concerted Recruitment of Constituents from Both Membrane Leaflets
title Ordered Lipid Domains Assemble via Concerted Recruitment of Constituents from Both Membrane Leaflets
title_full Ordered Lipid Domains Assemble via Concerted Recruitment of Constituents from Both Membrane Leaflets
title_fullStr Ordered Lipid Domains Assemble via Concerted Recruitment of Constituents from Both Membrane Leaflets
title_full_unstemmed Ordered Lipid Domains Assemble via Concerted Recruitment of Constituents from Both Membrane Leaflets
title_short Ordered Lipid Domains Assemble via Concerted Recruitment of Constituents from Both Membrane Leaflets
title_sort ordered lipid domains assemble via concerted recruitment of constituents from both membrane leaflets
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115998/
https://www.ncbi.nlm.nih.gov/pubmed/32216409
http://dx.doi.org/10.1103/PhysRevLett.124.108102
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