A comparison of three rapid diagnostic methods for the detection of rotavirus infection in calves

Three techniques for the detection of rotavirus in faecal samples from calves with neonatal gastroenteritis were compared. A preliminary study indicated that reverse passive haemagglutination (RPHA) was at least as sensitive as the enzyme-linked immunosorbent assay (ELISA). These two immunoassays we...

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Autores principales: Edwards, S., Chasey, D., Napthine, P., Banks, J., Hewitt-Taylor, C., Cranage, M.P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1987
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117104/
https://www.ncbi.nlm.nih.gov/pubmed/3027952
http://dx.doi.org/10.1016/0378-1135(87)90094-0
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author Edwards, S.
Chasey, D.
Napthine, P.
Banks, J.
Hewitt-Taylor, C.
Cranage, M.P.
author_facet Edwards, S.
Chasey, D.
Napthine, P.
Banks, J.
Hewitt-Taylor, C.
Cranage, M.P.
author_sort Edwards, S.
collection PubMed
description Three techniques for the detection of rotavirus in faecal samples from calves with neonatal gastroenteritis were compared. A preliminary study indicated that reverse passive haemagglutination (RPHA) was at least as sensitive as the enzyme-linked immunosorbent assay (ELISA). These two immunoassays were compared with the detection of viral RNA by polyacrylamide gel electrophoresis (PAGE) on 209 field samples. Of the 77 samples in which at least one test gave a positive result, 69 were positive by both RPHA and PAGE, but only 49 were also positive by ELISA, indicating a lower sensitivity for the latter test. The overall agreement between RPHA and PAGE was 96%. The reasons for the discrepancies between the tests are discussed.
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spelling pubmed-71171042020-04-02 A comparison of three rapid diagnostic methods for the detection of rotavirus infection in calves Edwards, S. Chasey, D. Napthine, P. Banks, J. Hewitt-Taylor, C. Cranage, M.P. Vet Microbiol Article Three techniques for the detection of rotavirus in faecal samples from calves with neonatal gastroenteritis were compared. A preliminary study indicated that reverse passive haemagglutination (RPHA) was at least as sensitive as the enzyme-linked immunosorbent assay (ELISA). These two immunoassays were compared with the detection of viral RNA by polyacrylamide gel electrophoresis (PAGE) on 209 field samples. Of the 77 samples in which at least one test gave a positive result, 69 were positive by both RPHA and PAGE, but only 49 were also positive by ELISA, indicating a lower sensitivity for the latter test. The overall agreement between RPHA and PAGE was 96%. The reasons for the discrepancies between the tests are discussed. Published by Elsevier B.V. 1987-01 2002-11-13 /pmc/articles/PMC7117104/ /pubmed/3027952 http://dx.doi.org/10.1016/0378-1135(87)90094-0 Text en Copyright © 1987 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Edwards, S.
Chasey, D.
Napthine, P.
Banks, J.
Hewitt-Taylor, C.
Cranage, M.P.
A comparison of three rapid diagnostic methods for the detection of rotavirus infection in calves
title A comparison of three rapid diagnostic methods for the detection of rotavirus infection in calves
title_full A comparison of three rapid diagnostic methods for the detection of rotavirus infection in calves
title_fullStr A comparison of three rapid diagnostic methods for the detection of rotavirus infection in calves
title_full_unstemmed A comparison of three rapid diagnostic methods for the detection of rotavirus infection in calves
title_short A comparison of three rapid diagnostic methods for the detection of rotavirus infection in calves
title_sort comparison of three rapid diagnostic methods for the detection of rotavirus infection in calves
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117104/
https://www.ncbi.nlm.nih.gov/pubmed/3027952
http://dx.doi.org/10.1016/0378-1135(87)90094-0
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