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Longitudinal surveillance of rotavirus A genotypes circulating in a high milk yield dairy cattle herd after the introduction of a rotavirus vaccine

Worldwide, neonatal diarrhea is one of the most important health issues affecting dairy calves, and rotavirus A (RVA) is one of its primary causes. Among the measures to mitigate the risk of diarrhea outbreaks, cow vaccination stands out as one of the most important. However, the immune pressure res...

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Autores principales: Fritzen, Juliana T.T., Oliveira, Marcos V., Lorenzetti, Elis, Miyabe, Flávia M., Viziack, Mariana P., Rodrigues, Carlos A., Ayres, Henderson, Alfieri, Alice F., Alfieri, Amauri A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117106/
https://www.ncbi.nlm.nih.gov/pubmed/30827398
http://dx.doi.org/10.1016/j.vetmic.2019.02.022
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author Fritzen, Juliana T.T.
Oliveira, Marcos V.
Lorenzetti, Elis
Miyabe, Flávia M.
Viziack, Mariana P.
Rodrigues, Carlos A.
Ayres, Henderson
Alfieri, Alice F.
Alfieri, Amauri A.
author_facet Fritzen, Juliana T.T.
Oliveira, Marcos V.
Lorenzetti, Elis
Miyabe, Flávia M.
Viziack, Mariana P.
Rodrigues, Carlos A.
Ayres, Henderson
Alfieri, Alice F.
Alfieri, Amauri A.
author_sort Fritzen, Juliana T.T.
collection PubMed
description Worldwide, neonatal diarrhea is one of the most important health issues affecting dairy calves, and rotavirus A (RVA) is one of its primary causes. Among the measures to mitigate the risk of diarrhea outbreaks, cow vaccination stands out as one of the most important. However, the immune pressure resulting from routine vaccination may be able to select specific G and P genotypes in RVA field strains. This study aimed to determine the frequency and intensity of neonatal diarrhea and the incidence of RVA and attempted to monitor the G and P genotypes present in the RVA strains circulating in a high milk yield cattle herd vaccinated with RVA G6P[5] strain. Fecal samples (n = 1220) from 122 Holstein heifer calves between 0–30 days old that were born from RVA-vaccinated cows were collected at 10 different time points, regardless of the presence or absence of diarrhea. The presence of RVA in fecal samples was determined by the polyacrylamide gel electrophoresis (PAGE) technique and confirmed by reverse transcription polymerase chain reaction (RT-PCR). G and P amplicons from 10 RVA-positive fecal samples from calves of different ages and collections were subjected to nucleotide sequencing. The proportion of the calves and fecal samples that were positive for RVA were 62.3% (76/122) and 8.1% (99/1220), respectively. Using sequence analysis, all 10 RVA field strains presented genotype G10P[11]. The protection of G6P[5] vaccination is clear, as this genotype was not detected in this study, and it is known that vaccination against RVA reduces the incidence of diarrhea independent of genotype involved. This result demonstrates the importance of epidemiological monitoring of RVA genotypes circulating in vaccinated dairy cattle herds to the early detection of new potential pathogenic RVA strains.
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spelling pubmed-71171062020-04-02 Longitudinal surveillance of rotavirus A genotypes circulating in a high milk yield dairy cattle herd after the introduction of a rotavirus vaccine Fritzen, Juliana T.T. Oliveira, Marcos V. Lorenzetti, Elis Miyabe, Flávia M. Viziack, Mariana P. Rodrigues, Carlos A. Ayres, Henderson Alfieri, Alice F. Alfieri, Amauri A. Vet Microbiol Article Worldwide, neonatal diarrhea is one of the most important health issues affecting dairy calves, and rotavirus A (RVA) is one of its primary causes. Among the measures to mitigate the risk of diarrhea outbreaks, cow vaccination stands out as one of the most important. However, the immune pressure resulting from routine vaccination may be able to select specific G and P genotypes in RVA field strains. This study aimed to determine the frequency and intensity of neonatal diarrhea and the incidence of RVA and attempted to monitor the G and P genotypes present in the RVA strains circulating in a high milk yield cattle herd vaccinated with RVA G6P[5] strain. Fecal samples (n = 1220) from 122 Holstein heifer calves between 0–30 days old that were born from RVA-vaccinated cows were collected at 10 different time points, regardless of the presence or absence of diarrhea. The presence of RVA in fecal samples was determined by the polyacrylamide gel electrophoresis (PAGE) technique and confirmed by reverse transcription polymerase chain reaction (RT-PCR). G and P amplicons from 10 RVA-positive fecal samples from calves of different ages and collections were subjected to nucleotide sequencing. The proportion of the calves and fecal samples that were positive for RVA were 62.3% (76/122) and 8.1% (99/1220), respectively. Using sequence analysis, all 10 RVA field strains presented genotype G10P[11]. The protection of G6P[5] vaccination is clear, as this genotype was not detected in this study, and it is known that vaccination against RVA reduces the incidence of diarrhea independent of genotype involved. This result demonstrates the importance of epidemiological monitoring of RVA genotypes circulating in vaccinated dairy cattle herds to the early detection of new potential pathogenic RVA strains. Elsevier B.V. 2019-03 2019-02-18 /pmc/articles/PMC7117106/ /pubmed/30827398 http://dx.doi.org/10.1016/j.vetmic.2019.02.022 Text en © 2019 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Fritzen, Juliana T.T.
Oliveira, Marcos V.
Lorenzetti, Elis
Miyabe, Flávia M.
Viziack, Mariana P.
Rodrigues, Carlos A.
Ayres, Henderson
Alfieri, Alice F.
Alfieri, Amauri A.
Longitudinal surveillance of rotavirus A genotypes circulating in a high milk yield dairy cattle herd after the introduction of a rotavirus vaccine
title Longitudinal surveillance of rotavirus A genotypes circulating in a high milk yield dairy cattle herd after the introduction of a rotavirus vaccine
title_full Longitudinal surveillance of rotavirus A genotypes circulating in a high milk yield dairy cattle herd after the introduction of a rotavirus vaccine
title_fullStr Longitudinal surveillance of rotavirus A genotypes circulating in a high milk yield dairy cattle herd after the introduction of a rotavirus vaccine
title_full_unstemmed Longitudinal surveillance of rotavirus A genotypes circulating in a high milk yield dairy cattle herd after the introduction of a rotavirus vaccine
title_short Longitudinal surveillance of rotavirus A genotypes circulating in a high milk yield dairy cattle herd after the introduction of a rotavirus vaccine
title_sort longitudinal surveillance of rotavirus a genotypes circulating in a high milk yield dairy cattle herd after the introduction of a rotavirus vaccine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117106/
https://www.ncbi.nlm.nih.gov/pubmed/30827398
http://dx.doi.org/10.1016/j.vetmic.2019.02.022
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