Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of the coronavirus-like agent in feces of pigs naturally affected with porcine epidemic diarrhea (PED) or experimentally infected with the CV777 isolate. The assay was specific and more sensitive than electron microscopy. A...

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Detalles Bibliográficos
Autores principales: Callebaut, P., Debouck, P., Pensaert, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1982
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117127/
https://www.ncbi.nlm.nih.gov/pubmed/7179716
http://dx.doi.org/10.1016/0378-1135(82)90009-8
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author Callebaut, P.
Debouck, P.
Pensaert, M.
author_facet Callebaut, P.
Debouck, P.
Pensaert, M.
author_sort Callebaut, P.
collection PubMed
description An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of the coronavirus-like agent in feces of pigs naturally affected with porcine epidemic diarrhea (PED) or experimentally infected with the CV777 isolate. The assay was specific and more sensitive than electron microscopy. An ELISA blocking assay is described for the detection and titration of antibodies. Specific antibody formation was demonstrated in pigs experimentally infected with CV777 and in swine naturally affected with PED.
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spelling pubmed-71171272020-04-02 Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea Callebaut, P. Debouck, P. Pensaert, M. Vet Microbiol Article An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of the coronavirus-like agent in feces of pigs naturally affected with porcine epidemic diarrhea (PED) or experimentally infected with the CV777 isolate. The assay was specific and more sensitive than electron microscopy. An ELISA blocking assay is described for the detection and titration of antibodies. Specific antibody formation was demonstrated in pigs experimentally infected with CV777 and in swine naturally affected with PED. Published by Elsevier B.V. 1982-09 2002-11-13 /pmc/articles/PMC7117127/ /pubmed/7179716 http://dx.doi.org/10.1016/0378-1135(82)90009-8 Text en Copyright © 1982 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Callebaut, P.
Debouck, P.
Pensaert, M.
Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea
title Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea
title_full Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea
title_fullStr Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea
title_full_unstemmed Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea
title_short Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea
title_sort enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117127/
https://www.ncbi.nlm.nih.gov/pubmed/7179716
http://dx.doi.org/10.1016/0378-1135(82)90009-8
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AT debouckp enzymelinkedimmunosorbentassayforthedetectionofthecoronaviruslikeagentanditsantibodiesinpigswithporcineepidemicdiarrhea
AT pensaertm enzymelinkedimmunosorbentassayforthedetectionofthecoronaviruslikeagentanditsantibodiesinpigswithporcineepidemicdiarrhea

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