Cargando…

Sensitive detection and typing of porcine reproductive and respiratory syndrome virus by RT-PCR amplification of whole viral genes

Following the recent use of a live vaccine against porcine reproductive and respiratory syndrome virus (PRRSV) in Denmark, both American (vaccine) and European-type PRRSV now coexist in Danish herds. This situation highlighted a requirement for supplementary tests for precise virus-typing. As a resu...

Descripción completa

Detalles Bibliográficos
Autores principales:	Oleksiewicz, M.B, Bøtner, A, Madsen, K.G, Storgaard, T
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier Science B.V. 1998
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117142/ https://www.ncbi.nlm.nih.gov/pubmed/9874099 http://dx.doi.org/10.1016/S0378-1135(98)00254-5

Ejemplares similares

Experimental inoculation of late term pregnant sows with a field isolate of porcine reproductive and respiratory syndrome vaccine-derived virus
por: Nielsen, J, et al.
Publicado: (2002)

Determination of 5′-leader sequences from radically disparate strains of porcine reproductive and respiratory syndrome virus reveals the presence of highly conserved sequence motifs
por: Oleksiewicz, M. B., et al.
Publicado: (2014)

Detection and Typing of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus by Multiplex Real-Time RT-PCR
por: Wernike, Kerstin, et al.
Publicado: (2012)

A Multiplex RT-PCR Assay to Detect and Discriminate Porcine Reproductive and Respiratory Syndrome Viruses in Clinical Specimens
por: Yang, Keli, et al.
Publicado: (2017)

Evaluation of a Porcine Endogenous Reference Gene (Internal Sample Control) in a Porcine Reproductive and Respiratory Syndrome Virus RT-qPCR
por: Munguía-Ramírez, Berenice, et al.
Publicado: (2023)

Development and Implementation of a Quadruple RT-qPCR Method for the Identification of Porcine Reproductive and Respiratory Syndrome Virus Strains
por: Ruan, Shengnan, et al.
Publicado: (2023)

Multiplex Real-Time RT-PCR Assays for Detection and Differentiation of Porcine Enteric Coronaviruses
por: Lazov, Christina M., et al.
Publicado: (2023)

The development of a rapid SYBR one step real-time RT-PCR for detection of porcine reproductive and respiratory syndrome virus
por: Tian, Hong, et al.
Publicado: (2010)

Phi29 polymerase based random amplification of viral RNA as an alternative to random RT-PCR
por: Berthet, Nicolas, et al.
Publicado: (2008)

Detection of transmissible gastroenteritis virus by RT-PCR and differentiation from porcine respiratory coronavirus
por: Paton, David, et al.
Publicado: (1997)

Genetic diversity of porcine reproductive and respiratory syndrome virus and evaluation of three one-step real-time RT-PCR assays in Korea
por: Shin, Go-Eun, et al.
Publicado: (2022)

Development of a Real-Time TaqMan RT-PCR Assay for the Detection of NADC34-like Porcine Reproductive and Respiratory Syndrome Virus
por: Tu, Teng, et al.
Publicado: (2023)

Application of a SYBR® Green One Step Real-time RT-PCR Assay to Detect Type 1 Porcine Reproductive and Respiratory Syndrome Virus
por: ISEKI, Hiroshi, et al.
Publicado: (2014)

Multiplex nested RT-PCR for the detection of porcine enteric viruses
por: Salem, Abid Nabil Ben, et al.
Publicado: (2010)

Clinical COVID-19 diagnostic methods: Comparison of reverse transcription loop-mediated isothermal amplification (RT-LAMP) and quantitative RT-PCR (qRT-PCR)
por: Kitajima, Heita, et al.
Publicado: (2021)

Development of a Multiplex Crystal Digital RT-PCR for Differential Detection of Classical, Highly Pathogenic, and NADC30-like Porcine Reproductive and Respiratory Syndrome Virus
por: Long, Feng, et al.
Publicado: (2023)

Real-time RT-PCR detection of 12 respiratory viral infections in four triplex reactions
por: Gunson, R.N., et al.
Publicado: (2005)

Development of a multiplex qRT-PCR assay for detection of African swine fever virus, classical swine fever virus and porcine reproductive and respiratory syndrome virus
por: Chen, Yating, et al.
Publicado: (2021)

Nitric oxide is elicited and inhibits viral replication in pigs infected with porcine respiratory coronavirus but not porcine reproductive and respiratory syndrome virus
por: Jung, Kwonil, et al.
Publicado: (2010)

Direct RT-PCR amplification of SARS-CoV-2 from clinical samples using a concentrated viral lysis-amplification buffer prepared with IGEPAL-630
por: Castellanos-Gonzalez, Alejandro, et al.
Publicado: (2021)

Sensitivity and reproducibility of standardized-competitive RT-PCR for transcript quantification and its comparison with real time RT-PCR
por: Pagliarulo, Vincenzo, et al.
Publicado: (2004)

Use of a Multiplex RT-PCR Assay for Simultaneous Detection of the North American Genotype Porcine Reproductive and Respiratory Syndrome Virus, Swine Influenza Virus and Japanese Encephalitis Virus
por: CHEN, Hong-ying, et al.
Publicado: (2010)

Multiplex RT-PCR for detecting nineteen respiratory viruses
por: Mahony, J., et al.
Publicado: (2006)

Development of a Crystal Digital RT-PCR for the Detection of Atypical Porcine Pestivirus
por: Liu, Huixin, et al.
Publicado: (2023)

Web-based LinRegPCR: application for the visualization and analysis of (RT)-qPCR amplification and melting data
por: Untergasser, Andreas, et al.
Publicado: (2021)

Respiratory virus multiplex RT-PCR assay sensitivities and influence factors in hospitalized children with lower respiratory tract infections
por: Deng, Jikui, et al.
Publicado: (2013)

Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
por: Paiva, Marcelo Henrique Santos, et al.
Publicado: (2017)

Assessing sensitivity and reproducibility of RT-ddPCR and RT-qPCR for the quantification of SARS-CoV-2 in wastewater
por: Ciesielski, Mark, et al.
Publicado: (2021)

Reverse transcription loop-mediated isothermal amplification for the detection of highly pathogenic porcine reproductive and respiratory syndrome virus
por: Chen, Hao-tai, et al.
Publicado: (2008)

Development of a Quadruplex RT-qPCR for the Detection of Porcine Rotaviruses and the Phylogenetic Analysis of Porcine RVH in China
por: Shi, Kaichuang, et al.
Publicado: (2023)

A duplex nested RT-PCR method for monitoring porcine epidemic diarrhea virus and porcine delta-coronavirus
por: Li, Chun Qi, et al.
Publicado: (2023)

Real-time RT-PCR detection of Bovine Viral Diarrhoea virus in whole blood using an external RNA reference
por: Young, N.J., et al.
Publicado: (2006)

Reproduction of postweaning multisystemic wasting syndrome (PMWS) in immunostimulated and non-immunostimulated 3-week-old piglets experimentally infected with porcine circovirus type 2 (PCV2)
por: Ladekjær-Mikkelsen, A.-S, et al.
Publicado: (2002)

Viral culture and immunofluorescence for the detection of SARS-CoV-2 infectivity in RT-PCR positive respiratory samples
por: Berengua, Carla, et al.
Publicado: (2022)

Porcine epidemic diarrhea virus: Viral RNA detection and quantification using a validated one-step real time RT-PCR
por: Bigault, Lionel, et al.
Publicado: (2020)

Engineering of a thermostable viral polymerase using metagenome-derived diversity for highly sensitive and specific RT-PCR
por: Heller, Ryan C, et al.
Publicado: (2019)

Sensitive Detection of SARS Coronavirus RNA by a Novel Asymmetric Multiplex Nested RT-PCR Amplification Coupled With Oligonucleotide Microarray Hybridization
por: Zhang, Zhi-wei, et al.
Publicado: (2005)

Validation of reference genes for quantitative RT-PCR studies in porcine oocytes and preimplantation embryos
por: Kuijk, Ewart W, et al.
Publicado: (2007)

Development of a multiplex RT-PCR method for the detection of four porcine enteric coronaviruses
por: Niu, Jia-Wei, et al.
Publicado: (2022)

A Quadruplex qRT-PCR for Differential Detection of Four Porcine Enteric Coronaviruses
por: Zhou, Hongjin, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_lns7l5efudrfpjdui8kpm2mbfq