Detection of porcine rotavirus in stools by a latex agglutination test

We developed a simple agglutination test for the detection of porcine rotavirus in stools from pigs with diarrhea. The virus was detected with high sensitivity and specificity by a slide agglutination test using latex particles coated with antibody against the porcine rotavirus strain OSU (LA-antiOS...

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Detalles Bibliográficos
Autores principales: Sanekata, Takeshi, Kishimoto, Eiichi, Sato, Kunihiko, Honma, Hitoshi, Otsuki, Koichi, Tsubokura, Misao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1991
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117147/
https://www.ncbi.nlm.nih.gov/pubmed/1652863
http://dx.doi.org/10.1016/0378-1135(91)90151-5
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author Sanekata, Takeshi
Kishimoto, Eiichi
Sato, Kunihiko
Honma, Hitoshi
Otsuki, Koichi
Tsubokura, Misao
author_facet Sanekata, Takeshi
Kishimoto, Eiichi
Sato, Kunihiko
Honma, Hitoshi
Otsuki, Koichi
Tsubokura, Misao
author_sort Sanekata, Takeshi
collection PubMed
description We developed a simple agglutination test for the detection of porcine rotavirus in stools from pigs with diarrhea. The virus was detected with high sensitivity and specificity by a slide agglutination test using latex particles coated with antibody against the porcine rotavirus strain OSU (LA-antiOSU). The agglutination of LA-antiOSU with OSU on a glass slide evident macroscopically within 2 min. The sensitivity of this latex agglutination (LA) test was four times higher than that of the electron microscope method. The LA test is available for the rapid diagnosis of porcine rotavirus infections.
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spelling pubmed-71171472020-04-02 Detection of porcine rotavirus in stools by a latex agglutination test Sanekata, Takeshi Kishimoto, Eiichi Sato, Kunihiko Honma, Hitoshi Otsuki, Koichi Tsubokura, Misao Vet Microbiol Article We developed a simple agglutination test for the detection of porcine rotavirus in stools from pigs with diarrhea. The virus was detected with high sensitivity and specificity by a slide agglutination test using latex particles coated with antibody against the porcine rotavirus strain OSU (LA-antiOSU). The agglutination of LA-antiOSU with OSU on a glass slide evident macroscopically within 2 min. The sensitivity of this latex agglutination (LA) test was four times higher than that of the electron microscope method. The LA test is available for the rapid diagnosis of porcine rotavirus infections. Published by Elsevier B.V. 1991-05 2002-11-05 /pmc/articles/PMC7117147/ /pubmed/1652863 http://dx.doi.org/10.1016/0378-1135(91)90151-5 Text en Copyright © 1991 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Sanekata, Takeshi
Kishimoto, Eiichi
Sato, Kunihiko
Honma, Hitoshi
Otsuki, Koichi
Tsubokura, Misao
Detection of porcine rotavirus in stools by a latex agglutination test
title Detection of porcine rotavirus in stools by a latex agglutination test
title_full Detection of porcine rotavirus in stools by a latex agglutination test
title_fullStr Detection of porcine rotavirus in stools by a latex agglutination test
title_full_unstemmed Detection of porcine rotavirus in stools by a latex agglutination test
title_short Detection of porcine rotavirus in stools by a latex agglutination test
title_sort detection of porcine rotavirus in stools by a latex agglutination test
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117147/
https://www.ncbi.nlm.nih.gov/pubmed/1652863
http://dx.doi.org/10.1016/0378-1135(91)90151-5
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