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Characterisation of bubaline coronavirus strains associated with gastroenteritis in water buffalo (Bubalus bubalis) calves

Recently, a coronavirus strain (179/07-11) was isolated from water buffalo (Bubalus bubalis) and the virus which displayed a strict genetic and biological relatedness with bovine coronavirus (BCoV) was referred to as bubaline coronavirus (BuCoV). Here, we report the characterisation of four BuCoVs s...

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Autores principales: Decaro, Nicola, Cirone, Francesco, Mari, Viviana, Nava, Donatella, Tinelli, Antonella, Elia, Gabriella, Di Sarno, Alessandra, Martella, Vito, Colaianni, Maria Loredana, Aprea, Giuseppe, Tempesta, Maria, Buonavoglia, Canio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117158/
https://www.ncbi.nlm.nih.gov/pubmed/20483547
http://dx.doi.org/10.1016/j.vetmic.2010.04.010
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author Decaro, Nicola
Cirone, Francesco
Mari, Viviana
Nava, Donatella
Tinelli, Antonella
Elia, Gabriella
Di Sarno, Alessandra
Martella, Vito
Colaianni, Maria Loredana
Aprea, Giuseppe
Tempesta, Maria
Buonavoglia, Canio
author_facet Decaro, Nicola
Cirone, Francesco
Mari, Viviana
Nava, Donatella
Tinelli, Antonella
Elia, Gabriella
Di Sarno, Alessandra
Martella, Vito
Colaianni, Maria Loredana
Aprea, Giuseppe
Tempesta, Maria
Buonavoglia, Canio
author_sort Decaro, Nicola
collection PubMed
description Recently, a coronavirus strain (179/07-11) was isolated from water buffalo (Bubalus bubalis) and the virus which displayed a strict genetic and biological relatedness with bovine coronavirus (BCoV) was referred to as bubaline coronavirus (BuCoV). Here, we report the characterisation of four BuCoVs strains identified in the faeces or intestinal contents of water buffalo calves with acute gastroenteritis. Single BuCoV infections were detected in all but one cases from which two clostridia species were also isolated. Sequence and phylogenetic analyses of the 5′ end of the spike-protein gene showed that three BuCoVs were closely related to the prototype strain 179/07-11, whereas the fourth isolate (339/08-C) displayed a higher genetic identity to recent BCoV reference strains. Three strains adapted to the in vitro grow on human rectal tumour cells were also evaluated for their ability to replicate in a bovine cell line (Madin Darby bovine kidney) and to cause haemagglutination of chicken erythrocytes and all displayed biological properties similar to those already described for the prototype BuCoV. The present report shows that albeit genetically heterogeneous, the different BuCoV strains possess a common biological pattern which is different from most BCoV and BCoV-like isolates.
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spelling pubmed-71171582020-04-02 Characterisation of bubaline coronavirus strains associated with gastroenteritis in water buffalo (Bubalus bubalis) calves Decaro, Nicola Cirone, Francesco Mari, Viviana Nava, Donatella Tinelli, Antonella Elia, Gabriella Di Sarno, Alessandra Martella, Vito Colaianni, Maria Loredana Aprea, Giuseppe Tempesta, Maria Buonavoglia, Canio Vet Microbiol Article Recently, a coronavirus strain (179/07-11) was isolated from water buffalo (Bubalus bubalis) and the virus which displayed a strict genetic and biological relatedness with bovine coronavirus (BCoV) was referred to as bubaline coronavirus (BuCoV). Here, we report the characterisation of four BuCoVs strains identified in the faeces or intestinal contents of water buffalo calves with acute gastroenteritis. Single BuCoV infections were detected in all but one cases from which two clostridia species were also isolated. Sequence and phylogenetic analyses of the 5′ end of the spike-protein gene showed that three BuCoVs were closely related to the prototype strain 179/07-11, whereas the fourth isolate (339/08-C) displayed a higher genetic identity to recent BCoV reference strains. Three strains adapted to the in vitro grow on human rectal tumour cells were also evaluated for their ability to replicate in a bovine cell line (Madin Darby bovine kidney) and to cause haemagglutination of chicken erythrocytes and all displayed biological properties similar to those already described for the prototype BuCoV. The present report shows that albeit genetically heterogeneous, the different BuCoV strains possess a common biological pattern which is different from most BCoV and BCoV-like isolates. Elsevier B.V. 2010-10-26 2010-04-21 /pmc/articles/PMC7117158/ /pubmed/20483547 http://dx.doi.org/10.1016/j.vetmic.2010.04.010 Text en Copyright © 2010 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Decaro, Nicola
Cirone, Francesco
Mari, Viviana
Nava, Donatella
Tinelli, Antonella
Elia, Gabriella
Di Sarno, Alessandra
Martella, Vito
Colaianni, Maria Loredana
Aprea, Giuseppe
Tempesta, Maria
Buonavoglia, Canio
Characterisation of bubaline coronavirus strains associated with gastroenteritis in water buffalo (Bubalus bubalis) calves
title Characterisation of bubaline coronavirus strains associated with gastroenteritis in water buffalo (Bubalus bubalis) calves
title_full Characterisation of bubaline coronavirus strains associated with gastroenteritis in water buffalo (Bubalus bubalis) calves
title_fullStr Characterisation of bubaline coronavirus strains associated with gastroenteritis in water buffalo (Bubalus bubalis) calves
title_full_unstemmed Characterisation of bubaline coronavirus strains associated with gastroenteritis in water buffalo (Bubalus bubalis) calves
title_short Characterisation of bubaline coronavirus strains associated with gastroenteritis in water buffalo (Bubalus bubalis) calves
title_sort characterisation of bubaline coronavirus strains associated with gastroenteritis in water buffalo (bubalus bubalis) calves
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117158/
https://www.ncbi.nlm.nih.gov/pubmed/20483547
http://dx.doi.org/10.1016/j.vetmic.2010.04.010
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