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Antibody dynamics in BRSV-infected Danish dairy herds as determined by isotype-specific immunoglobulins

Using specific ELISAs, antibody levels of four different isotypes to bovine respiratory syncytial virus (BRSV) were determined in calves, following experimental BRSV infection. Most calves experienced an increase in the specific IgM and IgG1 titres about 6–10 days after infection with BRSV. The IgM...

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Autores principales: Uttenthal, Åse, Larsen, Lars Erik, Philipsen, Jens Stege, Tjørnehøj, Kirsten, Viuff, Birgitte, Nielsen, Knud Herby, Nielsen, Thomas Krogh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science B.V. 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117174/
https://www.ncbi.nlm.nih.gov/pubmed/11000530
http://dx.doi.org/10.1016/S0378-1135(00)00261-3
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author Uttenthal, Åse
Larsen, Lars Erik
Philipsen, Jens Stege
Tjørnehøj, Kirsten
Viuff, Birgitte
Nielsen, Knud Herby
Nielsen, Thomas Krogh
author_facet Uttenthal, Åse
Larsen, Lars Erik
Philipsen, Jens Stege
Tjørnehøj, Kirsten
Viuff, Birgitte
Nielsen, Knud Herby
Nielsen, Thomas Krogh
author_sort Uttenthal, Åse
collection PubMed
description Using specific ELISAs, antibody levels of four different isotypes to bovine respiratory syncytial virus (BRSV) were determined in calves, following experimental BRSV infection. Most calves experienced an increase in the specific IgM and IgG1 titres about 6–10 days after infection with BRSV. The IgM titre was transient showing positive titres for only 5–10 days, while specific IgG1 was present for a longer time. IgA was detected concomitantly with IgM but at a lower level. Production of IgG2 anti-BRSV antibodies was detected from 3 weeks after infection. In two closed herds, repeated blood samplings were performed on young stock to analyse maternal immunity. The passively transferred antibodies were mainly of the IgG1 isotype and the half-life of IgG1 to BRSV was estimated to be 26.6 days. One of the herds had an outbreak of enzootic pneumonia, diagnosed to be caused by BRSV. Furthermore, another herd with acute BRSV was followed by weekly blood samples in six calves; in both herds IgM and IgG1 was detected shortly after the appearance of clinical signs. Serum samples from 50 Danish dairy herds (453 samples) were tested for immunoglobulins of the isotypes IgG1, IgG2 and IgM. The presence of antibodies to BRSV was widespread and more than 54% of the samples had BRSV antibodies of both the IgG1 and IgG2 isotypes indicating a high herd prevalence to BRSV. Test samples from two herds out of 50 were free from all isotypes to BRSV.
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spelling pubmed-71171742020-04-02 Antibody dynamics in BRSV-infected Danish dairy herds as determined by isotype-specific immunoglobulins Uttenthal, Åse Larsen, Lars Erik Philipsen, Jens Stege Tjørnehøj, Kirsten Viuff, Birgitte Nielsen, Knud Herby Nielsen, Thomas Krogh Vet Microbiol Article Using specific ELISAs, antibody levels of four different isotypes to bovine respiratory syncytial virus (BRSV) were determined in calves, following experimental BRSV infection. Most calves experienced an increase in the specific IgM and IgG1 titres about 6–10 days after infection with BRSV. The IgM titre was transient showing positive titres for only 5–10 days, while specific IgG1 was present for a longer time. IgA was detected concomitantly with IgM but at a lower level. Production of IgG2 anti-BRSV antibodies was detected from 3 weeks after infection. In two closed herds, repeated blood samplings were performed on young stock to analyse maternal immunity. The passively transferred antibodies were mainly of the IgG1 isotype and the half-life of IgG1 to BRSV was estimated to be 26.6 days. One of the herds had an outbreak of enzootic pneumonia, diagnosed to be caused by BRSV. Furthermore, another herd with acute BRSV was followed by weekly blood samples in six calves; in both herds IgM and IgG1 was detected shortly after the appearance of clinical signs. Serum samples from 50 Danish dairy herds (453 samples) were tested for immunoglobulins of the isotypes IgG1, IgG2 and IgM. The presence of antibodies to BRSV was widespread and more than 54% of the samples had BRSV antibodies of both the IgG1 and IgG2 isotypes indicating a high herd prevalence to BRSV. Test samples from two herds out of 50 were free from all isotypes to BRSV. Elsevier Science B.V. 2000-10-20 2000-09-20 /pmc/articles/PMC7117174/ /pubmed/11000530 http://dx.doi.org/10.1016/S0378-1135(00)00261-3 Text en Copyright © 2000 Elsevier Science B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Uttenthal, Åse
Larsen, Lars Erik
Philipsen, Jens Stege
Tjørnehøj, Kirsten
Viuff, Birgitte
Nielsen, Knud Herby
Nielsen, Thomas Krogh
Antibody dynamics in BRSV-infected Danish dairy herds as determined by isotype-specific immunoglobulins
title Antibody dynamics in BRSV-infected Danish dairy herds as determined by isotype-specific immunoglobulins
title_full Antibody dynamics in BRSV-infected Danish dairy herds as determined by isotype-specific immunoglobulins
title_fullStr Antibody dynamics in BRSV-infected Danish dairy herds as determined by isotype-specific immunoglobulins
title_full_unstemmed Antibody dynamics in BRSV-infected Danish dairy herds as determined by isotype-specific immunoglobulins
title_short Antibody dynamics in BRSV-infected Danish dairy herds as determined by isotype-specific immunoglobulins
title_sort antibody dynamics in brsv-infected danish dairy herds as determined by isotype-specific immunoglobulins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117174/
https://www.ncbi.nlm.nih.gov/pubmed/11000530
http://dx.doi.org/10.1016/S0378-1135(00)00261-3
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