Cargando…
Comparative analysis of immune responses following experimental infection of pigs with European porcine reproductive and respiratory syndrome virus strains of differing virulence
Porcine reproductive and respiratory syndrome virus (PRRSV) is difficult to control due to a high mutation rate and the emergence of virulent strains. The objective of this study was to analyze the immunological and pathological responses after infection with the European subtype 3 strain Lena in co...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117209/ https://www.ncbi.nlm.nih.gov/pubmed/23036445 http://dx.doi.org/10.1016/j.vetmic.2012.09.013 |
_version_ | 1783514319349088256 |
---|---|
author | Weesendorp, Eefke Morgan, Sophie Stockhofe-Zurwieden, Norbert Graaf, Ditta J. Popma-De Graham, Simon P. Rebel, Johanna M.J. |
author_facet | Weesendorp, Eefke Morgan, Sophie Stockhofe-Zurwieden, Norbert Graaf, Ditta J. Popma-De Graham, Simon P. Rebel, Johanna M.J. |
author_sort | Weesendorp, Eefke |
collection | PubMed |
description | Porcine reproductive and respiratory syndrome virus (PRRSV) is difficult to control due to a high mutation rate and the emergence of virulent strains. The objective of this study was to analyze the immunological and pathological responses after infection with the European subtype 3 strain Lena in comparison to subtype 1 strains Belgium A and Lelystad-Ter Huurne (LV). Sixteen pigs were inoculated per strain, and sixteen pigs with PBS. At days 7 and 21 post-inoculation (p.i.), four pigs per group were immunized with an Aujeszky disease vaccine (ADV) to study the immune competence after PRRSV infection. Infection with the Lena strain resulted in fever and clinical signs. This was not observed in the Belgium A or LV-infected pigs. Infection with the Lena strain resulted in high virus titers in serum, low numbers of IFN-γ secreting cells, a change in leukocyte populations and a delayed antibody response to immunization with ADV. Levels of IL-1β, IFN-α, IL-10, IL-12, TNF-α and IFN-γ mRNA of the Lena-infected pigs were increased during the first week of infection. For pigs infected with the Belgium A or LV strain, the effects of infection on these parameters were less pronounced, although for the Belgium A-infected pigs, the level of the analyzed cytokines, except for TNF-α, and leukocyte populations were comparable to the Lena-infected pigs. These results suggest that while the outcome of infection for the three strains was comparable, with mostly clearance of viremia at day 33 p.i, differences in immune responses were observed, perhaps contributing to their virulence. |
format | Online Article Text |
id | pubmed-7117209 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71172092020-04-02 Comparative analysis of immune responses following experimental infection of pigs with European porcine reproductive and respiratory syndrome virus strains of differing virulence Weesendorp, Eefke Morgan, Sophie Stockhofe-Zurwieden, Norbert Graaf, Ditta J. Popma-De Graham, Simon P. Rebel, Johanna M.J. Vet Microbiol Article Porcine reproductive and respiratory syndrome virus (PRRSV) is difficult to control due to a high mutation rate and the emergence of virulent strains. The objective of this study was to analyze the immunological and pathological responses after infection with the European subtype 3 strain Lena in comparison to subtype 1 strains Belgium A and Lelystad-Ter Huurne (LV). Sixteen pigs were inoculated per strain, and sixteen pigs with PBS. At days 7 and 21 post-inoculation (p.i.), four pigs per group were immunized with an Aujeszky disease vaccine (ADV) to study the immune competence after PRRSV infection. Infection with the Lena strain resulted in fever and clinical signs. This was not observed in the Belgium A or LV-infected pigs. Infection with the Lena strain resulted in high virus titers in serum, low numbers of IFN-γ secreting cells, a change in leukocyte populations and a delayed antibody response to immunization with ADV. Levels of IL-1β, IFN-α, IL-10, IL-12, TNF-α and IFN-γ mRNA of the Lena-infected pigs were increased during the first week of infection. For pigs infected with the Belgium A or LV strain, the effects of infection on these parameters were less pronounced, although for the Belgium A-infected pigs, the level of the analyzed cytokines, except for TNF-α, and leukocyte populations were comparable to the Lena-infected pigs. These results suggest that while the outcome of infection for the three strains was comparable, with mostly clearance of viremia at day 33 p.i, differences in immune responses were observed, perhaps contributing to their virulence. Elsevier B.V. 2013-04-12 2012-09-19 /pmc/articles/PMC7117209/ /pubmed/23036445 http://dx.doi.org/10.1016/j.vetmic.2012.09.013 Text en Copyright © 2012 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Weesendorp, Eefke Morgan, Sophie Stockhofe-Zurwieden, Norbert Graaf, Ditta J. Popma-De Graham, Simon P. Rebel, Johanna M.J. Comparative analysis of immune responses following experimental infection of pigs with European porcine reproductive and respiratory syndrome virus strains of differing virulence |
title | Comparative analysis of immune responses following experimental infection of pigs with European porcine reproductive and respiratory syndrome virus strains of differing virulence |
title_full | Comparative analysis of immune responses following experimental infection of pigs with European porcine reproductive and respiratory syndrome virus strains of differing virulence |
title_fullStr | Comparative analysis of immune responses following experimental infection of pigs with European porcine reproductive and respiratory syndrome virus strains of differing virulence |
title_full_unstemmed | Comparative analysis of immune responses following experimental infection of pigs with European porcine reproductive and respiratory syndrome virus strains of differing virulence |
title_short | Comparative analysis of immune responses following experimental infection of pigs with European porcine reproductive and respiratory syndrome virus strains of differing virulence |
title_sort | comparative analysis of immune responses following experimental infection of pigs with european porcine reproductive and respiratory syndrome virus strains of differing virulence |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117209/ https://www.ncbi.nlm.nih.gov/pubmed/23036445 http://dx.doi.org/10.1016/j.vetmic.2012.09.013 |
work_keys_str_mv | AT weesendorpeefke comparativeanalysisofimmuneresponsesfollowingexperimentalinfectionofpigswitheuropeanporcinereproductiveandrespiratorysyndromevirusstrainsofdifferingvirulence AT morgansophie comparativeanalysisofimmuneresponsesfollowingexperimentalinfectionofpigswitheuropeanporcinereproductiveandrespiratorysyndromevirusstrainsofdifferingvirulence AT stockhofezurwiedennorbert comparativeanalysisofimmuneresponsesfollowingexperimentalinfectionofpigswitheuropeanporcinereproductiveandrespiratorysyndromevirusstrainsofdifferingvirulence AT graafdittajpopmade comparativeanalysisofimmuneresponsesfollowingexperimentalinfectionofpigswitheuropeanporcinereproductiveandrespiratorysyndromevirusstrainsofdifferingvirulence AT grahamsimonp comparativeanalysisofimmuneresponsesfollowingexperimentalinfectionofpigswitheuropeanporcinereproductiveandrespiratorysyndromevirusstrainsofdifferingvirulence AT rebeljohannamj comparativeanalysisofimmuneresponsesfollowingexperimentalinfectionofpigswitheuropeanporcinereproductiveandrespiratorysyndromevirusstrainsofdifferingvirulence |