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Fluorogenic RT–PCR assay (TaqMan) for detection and classification of bovine viral diarrhea virus

A single tube fluorogenic RT–PCR-based ‘TaqMan’ assay was developed for detection and classification of bovine viral diarrhea virus (BVDV). TaqMan–PCR was optimized to quantify BVD virus using the ABI PRISM 7700 sequence detection system and dual-labeled fluorogenic probes. Two different gene specif...

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Detalles Bibliográficos
Autores principales: Bhudevi, Bodreddigari, Weinstock, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science B.V. 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117215/
https://www.ncbi.nlm.nih.gov/pubmed/11524161
http://dx.doi.org/10.1016/S0378-1135(01)00390-X