Characterisation of rotavirus isolates from sub-clinically infected calves by genome profile analysis

Rotaviruses isolated from 43 sub-clinically infected calves from a single farm were analysed by genome profile analysis. The isolates showed genomic variation and eight different profiles were observed, including one which was atypical for Group A rotaviruses. The 3′ terminal labelling method for th...

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Autor principal: Pocock, D.H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1987
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117224/
https://www.ncbi.nlm.nih.gov/pubmed/3027953
http://dx.doi.org/10.1016/0378-1135(87)90095-2
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author Pocock, D.H.
author_facet Pocock, D.H.
author_sort Pocock, D.H.
collection PubMed
description Rotaviruses isolated from 43 sub-clinically infected calves from a single farm were analysed by genome profile analysis. The isolates showed genomic variation and eight different profiles were observed, including one which was atypical for Group A rotaviruses. The 3′ terminal labelling method for the analysis of genome profiles used in this study required only 1 ng of viral RNA, an increase of 1000-fold in sensitivity over ethidium bromide staining for detecting all rotavirus genome segments. However, dual infections involving two rotaviruses with distinct profiles could not be detected if the concentrations of the viruses differed by > 10-fold.
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spelling pubmed-71172242020-04-02 Characterisation of rotavirus isolates from sub-clinically infected calves by genome profile analysis Pocock, D.H. Vet Microbiol Article Rotaviruses isolated from 43 sub-clinically infected calves from a single farm were analysed by genome profile analysis. The isolates showed genomic variation and eight different profiles were observed, including one which was atypical for Group A rotaviruses. The 3′ terminal labelling method for the analysis of genome profiles used in this study required only 1 ng of viral RNA, an increase of 1000-fold in sensitivity over ethidium bromide staining for detecting all rotavirus genome segments. However, dual infections involving two rotaviruses with distinct profiles could not be detected if the concentrations of the viruses differed by > 10-fold. Published by Elsevier B.V. 1987-01 2002-11-13 /pmc/articles/PMC7117224/ /pubmed/3027953 http://dx.doi.org/10.1016/0378-1135(87)90095-2 Text en Copyright © 1987 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Pocock, D.H.
Characterisation of rotavirus isolates from sub-clinically infected calves by genome profile analysis
title Characterisation of rotavirus isolates from sub-clinically infected calves by genome profile analysis
title_full Characterisation of rotavirus isolates from sub-clinically infected calves by genome profile analysis
title_fullStr Characterisation of rotavirus isolates from sub-clinically infected calves by genome profile analysis
title_full_unstemmed Characterisation of rotavirus isolates from sub-clinically infected calves by genome profile analysis
title_short Characterisation of rotavirus isolates from sub-clinically infected calves by genome profile analysis
title_sort characterisation of rotavirus isolates from sub-clinically infected calves by genome profile analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117224/
https://www.ncbi.nlm.nih.gov/pubmed/3027953
http://dx.doi.org/10.1016/0378-1135(87)90095-2
work_keys_str_mv AT pocockdh characterisationofrotavirusisolatesfromsubclinicallyinfectedcalvesbygenomeprofileanalysis

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