Cargando…
Recombinant feline coronaviruses as vaccine candidates confer protection in SPF but not in conventional cats()
Feline infectious peritonitis virus (FIPV) is a major pathogen of Felidae. Despite the extensive efforts taken in the past decades, development of the “ideal” live attenuated FIPV vaccine was not successful yet. In the present study, we provide data of immunisation experiments with a recombinant FCo...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Authors. Published by Elsevier B.V.
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117248/ https://www.ncbi.nlm.nih.gov/pubmed/24513277 http://dx.doi.org/10.1016/j.vetmic.2013.10.015 |
_version_ | 1783514328254644224 |
---|---|
author | Bálint, Ádám Farsang, Attila Szeredi, Levente Zádori, Zoltán Belák, Sándor |
author_facet | Bálint, Ádám Farsang, Attila Szeredi, Levente Zádori, Zoltán Belák, Sándor |
author_sort | Bálint, Ádám |
collection | PubMed |
description | Feline infectious peritonitis virus (FIPV) is a major pathogen of Felidae. Despite the extensive efforts taken in the past decades, development of the “ideal” live attenuated FIPV vaccine was not successful yet. In the present study, we provide data of immunisation experiments with a recombinant FCoV pair differing only in the truncation (PBFIPV-DF-2) or completion (PBFIPV-DF-2-R3i) of their ORF3abc regions. In our previous in vivo studies, these viruses proved to show the characters of low virulent or avirulent FCoV phenotypes, respectively. Therefore, we hypothesised the ability of these viruses, as possible vaccine candidates, in conferring protection in specific pathogen free (SPF) Domestic Shorthair as well as in conventional purebred British Shorthair cats. In SPF cats, after two oronasal and two intramuscular vaccinations with two weeks intervals, both vaccine candidates provided 100% protection against lethal homologous challenge with the highly virulent FIPV DF-2 strain. In contrast, the conventional purebred British Shorthair cats did not develop protection when they were immunised with the same vaccination regimes. In these groups 100% of the PBFIPV-DF-2-R3i immunised animals developed antibody-dependent enhancement (ADE). Prolonged survival was observed in 40% of the animals, while 60% showed fulminant disease course. Genetic and more probably immunological differences between the SPF and non-SPF purebred kittens can explain the different outcome of the vaccination experiment. Our data highlight the diverse immune responses between SPF and conventional cats and suggest a decisive role of previous infection by heterologous causative agents in the outcome of the vaccination against FIP. |
format | Online Article Text |
id | pubmed-7117248 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | The Authors. Published by Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71172482020-04-02 Recombinant feline coronaviruses as vaccine candidates confer protection in SPF but not in conventional cats() Bálint, Ádám Farsang, Attila Szeredi, Levente Zádori, Zoltán Belák, Sándor Vet Microbiol Article Feline infectious peritonitis virus (FIPV) is a major pathogen of Felidae. Despite the extensive efforts taken in the past decades, development of the “ideal” live attenuated FIPV vaccine was not successful yet. In the present study, we provide data of immunisation experiments with a recombinant FCoV pair differing only in the truncation (PBFIPV-DF-2) or completion (PBFIPV-DF-2-R3i) of their ORF3abc regions. In our previous in vivo studies, these viruses proved to show the characters of low virulent or avirulent FCoV phenotypes, respectively. Therefore, we hypothesised the ability of these viruses, as possible vaccine candidates, in conferring protection in specific pathogen free (SPF) Domestic Shorthair as well as in conventional purebred British Shorthair cats. In SPF cats, after two oronasal and two intramuscular vaccinations with two weeks intervals, both vaccine candidates provided 100% protection against lethal homologous challenge with the highly virulent FIPV DF-2 strain. In contrast, the conventional purebred British Shorthair cats did not develop protection when they were immunised with the same vaccination regimes. In these groups 100% of the PBFIPV-DF-2-R3i immunised animals developed antibody-dependent enhancement (ADE). Prolonged survival was observed in 40% of the animals, while 60% showed fulminant disease course. Genetic and more probably immunological differences between the SPF and non-SPF purebred kittens can explain the different outcome of the vaccination experiment. Our data highlight the diverse immune responses between SPF and conventional cats and suggest a decisive role of previous infection by heterologous causative agents in the outcome of the vaccination against FIP. The Authors. Published by Elsevier B.V. 2014-03-14 2013-10-29 /pmc/articles/PMC7117248/ /pubmed/24513277 http://dx.doi.org/10.1016/j.vetmic.2013.10.015 Text en © 2013 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Bálint, Ádám Farsang, Attila Szeredi, Levente Zádori, Zoltán Belák, Sándor Recombinant feline coronaviruses as vaccine candidates confer protection in SPF but not in conventional cats() |
title | Recombinant feline coronaviruses as vaccine candidates confer protection in SPF but not in conventional cats() |
title_full | Recombinant feline coronaviruses as vaccine candidates confer protection in SPF but not in conventional cats() |
title_fullStr | Recombinant feline coronaviruses as vaccine candidates confer protection in SPF but not in conventional cats() |
title_full_unstemmed | Recombinant feline coronaviruses as vaccine candidates confer protection in SPF but not in conventional cats() |
title_short | Recombinant feline coronaviruses as vaccine candidates confer protection in SPF but not in conventional cats() |
title_sort | recombinant feline coronaviruses as vaccine candidates confer protection in spf but not in conventional cats() |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117248/ https://www.ncbi.nlm.nih.gov/pubmed/24513277 http://dx.doi.org/10.1016/j.vetmic.2013.10.015 |
work_keys_str_mv | AT balintadam recombinantfelinecoronavirusesasvaccinecandidatesconferprotectioninspfbutnotinconventionalcats AT farsangattila recombinantfelinecoronavirusesasvaccinecandidatesconferprotectioninspfbutnotinconventionalcats AT szeredilevente recombinantfelinecoronavirusesasvaccinecandidatesconferprotectioninspfbutnotinconventionalcats AT zadorizoltan recombinantfelinecoronavirusesasvaccinecandidatesconferprotectioninspfbutnotinconventionalcats AT belaksandor recombinantfelinecoronavirusesasvaccinecandidatesconferprotectioninspfbutnotinconventionalcats |