Enzyme-linked immunosorbent assay for the detection of canine coronavirus and its antibody in dogs

Two methods of enzyme-linked immunosorbent assay (ELISA) were developed for the diagnosis of canine coronavirus (CCV) infection in dogs. One ELISA, in which CCV-infected CRFK cell lysate is used as antigen, is for the detection and titration of antibody against CCV, and the other ELISA uses the doub...

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Detalles Bibliográficos
Autores principales: Tuchiya, Kotaro, Horimoto, Taisuke, Azetaka, Masayuki, Takahashi, Eiji, Konishi, Shin-ichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1991
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117253/
https://www.ncbi.nlm.nih.gov/pubmed/1850890
http://dx.doi.org/10.1016/0378-1135(91)90040-M
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author Tuchiya, Kotaro
Horimoto, Taisuke
Azetaka, Masayuki
Takahashi, Eiji
Konishi, Shin-ichiro
author_facet Tuchiya, Kotaro
Horimoto, Taisuke
Azetaka, Masayuki
Takahashi, Eiji
Konishi, Shin-ichiro
author_sort Tuchiya, Kotaro
collection PubMed
description Two methods of enzyme-linked immunosorbent assay (ELISA) were developed for the diagnosis of canine coronavirus (CCV) infection in dogs. One ELISA, in which CCV-infected CRFK cell lysate is used as antigen, is for the detection and titration of antibody against CCV, and the other ELISA uses the double antibody sandwich method for the detection of CCV antigen. The first ELISA procedure demonstrated antibody responses in dogs inoculated with CCV, as did the virus neutralization test; the second ELISA detected specific CCV antigen in feces and organ homogenates of inoculated dogs.
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spelling pubmed-71172532020-04-02 Enzyme-linked immunosorbent assay for the detection of canine coronavirus and its antibody in dogs Tuchiya, Kotaro Horimoto, Taisuke Azetaka, Masayuki Takahashi, Eiji Konishi, Shin-ichiro Vet Microbiol Article Two methods of enzyme-linked immunosorbent assay (ELISA) were developed for the diagnosis of canine coronavirus (CCV) infection in dogs. One ELISA, in which CCV-infected CRFK cell lysate is used as antigen, is for the detection and titration of antibody against CCV, and the other ELISA uses the double antibody sandwich method for the detection of CCV antigen. The first ELISA procedure demonstrated antibody responses in dogs inoculated with CCV, as did the virus neutralization test; the second ELISA detected specific CCV antigen in feces and organ homogenates of inoculated dogs. Published by Elsevier B.V. 1991-01 2002-12-10 /pmc/articles/PMC7117253/ /pubmed/1850890 http://dx.doi.org/10.1016/0378-1135(91)90040-M Text en Copyright © 1991 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Tuchiya, Kotaro
Horimoto, Taisuke
Azetaka, Masayuki
Takahashi, Eiji
Konishi, Shin-ichiro
Enzyme-linked immunosorbent assay for the detection of canine coronavirus and its antibody in dogs
title Enzyme-linked immunosorbent assay for the detection of canine coronavirus and its antibody in dogs
title_full Enzyme-linked immunosorbent assay for the detection of canine coronavirus and its antibody in dogs
title_fullStr Enzyme-linked immunosorbent assay for the detection of canine coronavirus and its antibody in dogs
title_full_unstemmed Enzyme-linked immunosorbent assay for the detection of canine coronavirus and its antibody in dogs
title_short Enzyme-linked immunosorbent assay for the detection of canine coronavirus and its antibody in dogs
title_sort enzyme-linked immunosorbent assay for the detection of canine coronavirus and its antibody in dogs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117253/
https://www.ncbi.nlm.nih.gov/pubmed/1850890
http://dx.doi.org/10.1016/0378-1135(91)90040-M
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