Replication of a bovine coronavirus in organ cultures of foetal trachea

A strain of bovine coronavirus (BC) adapted to tissue culture, was inoculated into organ cultures of bovine foetal trachea. Haemagglutinin in the fluid from infected organ cultures reached titres of 32 and characteristic coronavirus particles were observed electron microscopically. BC virus antigen...

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Autores principales: Stott, E.J., Thomas, L.H., Bridger, J.C., Jebbett, N.J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1976
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117282/
http://dx.doi.org/10.1016/0378-1135(76)90010-9
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author Stott, E.J.
Thomas, L.H.
Bridger, J.C.
Jebbett, N.J.
author_facet Stott, E.J.
Thomas, L.H.
Bridger, J.C.
Jebbett, N.J.
author_sort Stott, E.J.
collection PubMed
description A strain of bovine coronavirus (BC) adapted to tissue culture, was inoculated into organ cultures of bovine foetal trachea. Haemagglutinin in the fluid from infected organ cultures reached titres of 32 and characteristic coronavirus particles were observed electron microscopically. BC virus antigen was detected in frozen sections of the organ cultures by staining with fluorescent antibody. These data were evidence that BC virus replicated in organ cultures of respiratory tissue. The use of this technique for primary isolation of bovine coronaviruses from field material is discussed.
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spelling pubmed-71172822020-04-02 Replication of a bovine coronavirus in organ cultures of foetal trachea Stott, E.J. Thomas, L.H. Bridger, J.C. Jebbett, N.J. Vet Microbiol Article A strain of bovine coronavirus (BC) adapted to tissue culture, was inoculated into organ cultures of bovine foetal trachea. Haemagglutinin in the fluid from infected organ cultures reached titres of 32 and characteristic coronavirus particles were observed electron microscopically. BC virus antigen was detected in frozen sections of the organ cultures by staining with fluorescent antibody. These data were evidence that BC virus replicated in organ cultures of respiratory tissue. The use of this technique for primary isolation of bovine coronaviruses from field material is discussed. Published by Elsevier B.V. 1976-07 2002-11-12 /pmc/articles/PMC7117282/ http://dx.doi.org/10.1016/0378-1135(76)90010-9 Text en Copyright © 1976 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Stott, E.J.
Thomas, L.H.
Bridger, J.C.
Jebbett, N.J.
Replication of a bovine coronavirus in organ cultures of foetal trachea
title Replication of a bovine coronavirus in organ cultures of foetal trachea
title_full Replication of a bovine coronavirus in organ cultures of foetal trachea
title_fullStr Replication of a bovine coronavirus in organ cultures of foetal trachea
title_full_unstemmed Replication of a bovine coronavirus in organ cultures of foetal trachea
title_short Replication of a bovine coronavirus in organ cultures of foetal trachea
title_sort replication of a bovine coronavirus in organ cultures of foetal trachea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117282/
http://dx.doi.org/10.1016/0378-1135(76)90010-9
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AT jebbettnj replicationofabovinecoronavirusinorganculturesoffoetaltrachea

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