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Experimental infection of dogs with a novel strain of canine coronavirus causing systemic disease and lymphopenia
A pantropic canine coronavirus (CCoV) strain (CB/05) has been recently associated to a fatal outbreak of systemic disease in young dogs. We report the clinical, virological and serological findings in dogs experimentally infected with strain CB/05. The dogs, three 2.5-month-old and two 6-month-old p...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117318/ https://www.ncbi.nlm.nih.gov/pubmed/18031959 http://dx.doi.org/10.1016/j.vetmic.2007.10.008 |
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author | Decaro, Nicola Campolo, Marco Lorusso, Alessio Desario, Costantina Mari, Viviana Colaianni, Maria Loredana Elia, Gabriella Martella, Vito Buonavoglia, Canio |
author_facet | Decaro, Nicola Campolo, Marco Lorusso, Alessio Desario, Costantina Mari, Viviana Colaianni, Maria Loredana Elia, Gabriella Martella, Vito Buonavoglia, Canio |
author_sort | Decaro, Nicola |
collection | PubMed |
description | A pantropic canine coronavirus (CCoV) strain (CB/05) has been recently associated to a fatal outbreak of systemic disease in young dogs. We report the clinical, virological and serological findings in dogs experimentally infected with strain CB/05. The dogs, three 2.5-month-old and two 6-month-old pups, were successfully infected, shedding viral RNA with their faeces for the entire observation period (21 days) and displaying systemic clinical signs resembling those observed during the course of natural infection. Leucopenia (acute lymphopenia) occurred in all infected dogs, with values dropping below 60% of the initial counts. Considering the severity of the CB/05-induced disease, two of the youngest pups were euthanized for ethical reasons at days 8–9 postinfection, whereas the other pups underwent a slow but progressive improvement of their clinical status with complete recovery. At postmortem examination, remarkable lesions were observed in the internal organs of the euthanized pups, that tested positive for CCoV by real-time RT-PCR and virus isolation on cell cultures. All pups seroconverted for CCoV, as shown by the high optical density values and antibody titres detected by ELISA and virusneutralisation tests, respectively. The present study confirms that strain CB/05 is highly pathogenic for dogs, being able to induce a severe disease (and in some cases the death) even in experimental conditions. |
format | Online Article Text |
id | pubmed-7117318 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71173182020-04-02 Experimental infection of dogs with a novel strain of canine coronavirus causing systemic disease and lymphopenia Decaro, Nicola Campolo, Marco Lorusso, Alessio Desario, Costantina Mari, Viviana Colaianni, Maria Loredana Elia, Gabriella Martella, Vito Buonavoglia, Canio Vet Microbiol Article A pantropic canine coronavirus (CCoV) strain (CB/05) has been recently associated to a fatal outbreak of systemic disease in young dogs. We report the clinical, virological and serological findings in dogs experimentally infected with strain CB/05. The dogs, three 2.5-month-old and two 6-month-old pups, were successfully infected, shedding viral RNA with their faeces for the entire observation period (21 days) and displaying systemic clinical signs resembling those observed during the course of natural infection. Leucopenia (acute lymphopenia) occurred in all infected dogs, with values dropping below 60% of the initial counts. Considering the severity of the CB/05-induced disease, two of the youngest pups were euthanized for ethical reasons at days 8–9 postinfection, whereas the other pups underwent a slow but progressive improvement of their clinical status with complete recovery. At postmortem examination, remarkable lesions were observed in the internal organs of the euthanized pups, that tested positive for CCoV by real-time RT-PCR and virus isolation on cell cultures. All pups seroconverted for CCoV, as shown by the high optical density values and antibody titres detected by ELISA and virusneutralisation tests, respectively. The present study confirms that strain CB/05 is highly pathogenic for dogs, being able to induce a severe disease (and in some cases the death) even in experimental conditions. Elsevier B.V. 2008-04-30 2007-10-18 /pmc/articles/PMC7117318/ /pubmed/18031959 http://dx.doi.org/10.1016/j.vetmic.2007.10.008 Text en Copyright © 2007 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Decaro, Nicola Campolo, Marco Lorusso, Alessio Desario, Costantina Mari, Viviana Colaianni, Maria Loredana Elia, Gabriella Martella, Vito Buonavoglia, Canio Experimental infection of dogs with a novel strain of canine coronavirus causing systemic disease and lymphopenia |
title | Experimental infection of dogs with a novel strain of canine coronavirus causing systemic disease and lymphopenia |
title_full | Experimental infection of dogs with a novel strain of canine coronavirus causing systemic disease and lymphopenia |
title_fullStr | Experimental infection of dogs with a novel strain of canine coronavirus causing systemic disease and lymphopenia |
title_full_unstemmed | Experimental infection of dogs with a novel strain of canine coronavirus causing systemic disease and lymphopenia |
title_short | Experimental infection of dogs with a novel strain of canine coronavirus causing systemic disease and lymphopenia |
title_sort | experimental infection of dogs with a novel strain of canine coronavirus causing systemic disease and lymphopenia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117318/ https://www.ncbi.nlm.nih.gov/pubmed/18031959 http://dx.doi.org/10.1016/j.vetmic.2007.10.008 |
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