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Establishment of reverse genetics system for infectious bronchitis virus attenuated vaccine strain H120

Infectious bronchitis virus (IBV) strain H120 was successfully rescued as infectious clone by reverse genetics. Thirteen 1.5–2.8 kb fragments contiguously spanning the virus genome were amplified and cloned into pMD19-T. Transcription grade complete length cDNA was acquired by a modified “No See’m”...

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Detalles Bibliográficos
Autores principales:	Zhou, Ying Shun, Zhang, Yi, Wang, Hong Ning, Fan, Wen Qiao, Yang, Xin, Zhang, An Yun, Zeng, Fan Ya, Zhang, Zhi Kun, Cao, Hai Peng, Zeng, Cheng
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier B.V. 2013
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117355/ https://www.ncbi.nlm.nih.gov/pubmed/22999521 http://dx.doi.org/10.1016/j.vetmic.2012.08.013

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